| FullText URL | fulltext.pdf |
|---|---|
| Author | Ikeda, Shiori| Sato, Keita| Fujita, Hirofumi| Ono-Minagi, Hitomi| Miyaishi, Satoru| Nohno, Tsutomu| Ohuchi, Hideyo| |
| Keywords | Harderian gland Fgf10 haploinsufficiency mouse |
| Published Date | 2024-06-03 |
| Publication Title | Journal of Developmental Biology |
| Volume | volume12 |
| Issue | issue2 |
| Publisher | MDPI |
| Start Page | 16 |
| ISSN | 2221-3759 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2024 by the authors. |
| File Version | publisher |
| PubMed ID | 38921483 |
| DOI | 10.3390/jdb12020016 |
| Web of Science KeyUT | 001257698900001 |
| Related Url | isVersionOf https://doi.org/10.3390/jdb12020016 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Fujita, Hirofumi| Ando, Aoi| Mizusawa, Yohei| Ono, Mitsuaki| Hattori, Takako| Habuta, Munenori| Oohashi, Toshitaka| Kubota, Satoshi| Ohuchi, Hideyo| |
| Published Date | 2022-11-17 |
| Publication Title | Plos One |
| Volume | volume17 |
| Issue | issue11 |
| Publisher | Public Library Science |
| Start Page | e0277307 |
| ISSN | 1932-6203 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 Fujita et al. |
| File Version | publisher |
| PubMed ID | 36395281 |
| DOI | 10.1371/journal.pone.0277307 |
| Web of Science KeyUT | 000926013600060 |
| Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0277307 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Xue, Ruizhi| Lin, Wenfeng| Fujita, Hirofumi| Sun, Jingkai| Kinoshita, Rie| Ochiai, Kazuhiko| Futami, Junichiro| Watanabe, Masami| Ohuchi, Hideyo| Sakaguchi, Masakiyo| Tang, Zhengyan| Huang, Peng| Nasu, Yasutomo| Kumon, Hiromi| |
| Keywords | Dkk3/REIC fibrous sheath knock-out RNA-seq spermiation sperm motility |
| Published Date | 2022-01-31 |
| Publication Title | Genes |
| Volume | volume13 |
| Issue | issue2 |
| Publisher | MDPI |
| Start Page | 285 |
| ISSN | 2073-4425 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 by the authors. |
| File Version | publisher |
| PubMed ID | 35205329 |
| DOI | 10.3390/genes13020285 |
| Web of Science KeyUT | 000762788200001 |
| Related Url | isVersionOf https://doi.org/10.3390/genes13020285 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Habuta, Munenori| Yasue, Akihiro| Suzuki, Ken-Ichi T.| Fujita, Hirofumi| Sato, Keita| Kono, Hitomi| Takayama, Ayuko| Bando, Tetsuya| Miyaishi, Satoru| Oyadomari, Seiichi| Tanaka, Eiji| Ohuchi, Hideyo| |
| Published Date | 2020-10-15 |
| Publication Title | PLoS ONE |
| Volume | volume15 |
| Issue | issue10 |
| Publisher | Public Library of Science |
| Start Page | e0240333 |
| ISSN | 1932-6203 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2020 Habuta et al. |
| File Version | publisher |
| PubMed ID | 33057360 |
| DOI | 10.1371/journal.pone.0240333 |
| Web of Science KeyUT | 000581814700082 |
| Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0240333 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Kanzaki, Yuki| Fujita, Hirofumi| Sato, Keita| Hosokawa, Mio| Matsumae, Hiroshi| Shiraga, Fumio| Morizane, Yuki| Ohuchi, Hideyo| |
| Keywords | Kir7.1 KCNJ13 human-induced pluripotent cells retinal pigment epithelium phagocytosis |
| Published Date | 2020-05 |
| Publication Title | Investigative Ophthalmology & Visual Science |
| Volume | volume61 |
| Issue | issue5 |
| Publisher | Association for Research in Vision and Ophthalmology |
| Start Page | 38 |
| ISSN | 0146-0404 |
| NCID | AA00683736 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | Copyright 2020 The Authors |
| File Version | publisher |
| PubMed ID | 32437550 |
| DOI | 10.1167/iovs.61.5.38 |
| Web of Science KeyUT | 000540905500039 |
| Related Url | isVersionOf https://doi.org/10.1167/iovs.61.5.38 |
| JaLCDOI | 10.18926/AMO/59950 |
|---|---|
| FullText URL | 74_3_199.pdf |
| Author | Fujita, Hirofumi| Bando, Tetsuya| Oyadomari, Seiichi| Ochiai, Kazuhiko| Watanabe, Masami| Kumon, Hiromi| Ohuchi, Hideyo| |
| Abstract | Dickkopf 3 (Dkk3) is a secreted protein belonging to the Dkk family and encoded by the orthologous gene of REIC. Dkk3/REIC is expressed by mouse and human adrenal glands, but the understanding of its roles in this organ is still limited. To determine the functions of Dkk3 in the mouse adrenal gland, we first identified that the mouse Dkk3 protein is N-glycosylated in the adrenal gland as well as in the brain. We performed proteome analysis on adrenal glands from Dkk3-null mice, in which exons 5 and 6 of the Dkk3 gene are deleted. Twodimensional polyacrylamide gel electrophoresis of adrenal proteins from wild-type and Dkk3-null mice revealed 5 protein spots whose intensities were altered between the 2 genotypes. Mass spectrometry analysis of these spots identified binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) chaperone. To determine whether mouse Dkk3 is involved in the unfolded protein response (UPR), we carried out a reporter assay using ER-stress responsive elements. Forced expression of Dkk3 resulted in the induction of distinct levels of reporter expression, showing the UPR initiated by the ER membrane proteins of activating transcription factor 6 (ATF6) and inositol-requring enzyme 1 (IRE1). Thus, it is possible that Dkk3 is a physiological ER stressor in the mouse adrenal gland. |
| Keywords | Dkk3 knockout mouse adrenal gland glucose-regulated protein 78 proteome endoplasmic reticulum stress |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2020-06 |
| Volume | volume74 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 199 |
| End Page | 208 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2020 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 32577017 |
| Web of Science KeyUT | 000543363400003 |
| NAID | 120006862792 |
| Author | Kobuchi, Hirotsugu| Moriya, Koko| Ogino, Tetsuya| Fujita, Hirofumi| Inoue, Keiji| Shuin, Taro| Yasuda, Tatsuji| Utsumi, Kozo| Utsumi, Toshihiko| |
|---|---|
| Published Date | 2012-11-26 |
| Publication Title | PLoS ONE |
| Volume | volume7 |
| Issue | issue11 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/50408 |
|---|---|
| FullText URL | 67_3_153.pdf |
| Author | Yamamoto, Masanao| Fujita, Hirofumi| Katase, Naoki| Inoue, Keiji| Nagatsuka, Hitoshi| Utsumi, Kozo| Sasaki, Junzo| Ohuchi, Hideyo| |
| Abstract | Ever since protoporphyrin IX (PpIX) was discovered to accumulate preferentially in cancer cells after 5-aminolevulinic acid (ALA) treatment, photodynamic treatment or therapy (PDT) has been developed as an exciting new treatment option for cancer patients. However, the level of PpIX accumulation in oral cancer is fairly low and insufficient for PDT. Ferrochelatase (FECH) and ATP-binding cassette transporter G2 (ABCG2) are known to regulate PpIX accumulation. In addition, serum enhances PpIX export by ABCG2. We investigated here whether and how inhibitors of FECH and ABCG2 and their combination could improve PpIX accumulation and PDT efficacy in an oral cancer cell line in serum-containing medium. ABCG2 inhibitor and the combination of ABCG2 and FECH inhibitors increased PpIX in the presence of fetal bovine serum (FBS) in an oral cancer cell line. Analysis of ABCG2 gene silencing also revealed the involvement of ABCG2 in the regulation of PpIX accumulation. Inhibitors of FECH and ABCG2, and their combination increased the efficiency of ALA-PDT even in the presence of FBS. ALA-PDT-induced cell death was accompanied by apoptotic events and lipid peroxidation. These results suggest that accumulation of PpIX is determined by the activities of ABCG2 and FECH and that treatment with a combination of their inhibitors improves the efficacy of PDT for oral cancer, especially in the presence of serum. |
| Keywords | 5-aminolevulinic acid protoporphyrin IX oncology photodynamic therapy apoptosis |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-06 |
| Volume | volume67 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 153 |
| End Page | 164 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23804138 |
| Web of Science KeyUT | 000320747900004 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/50681 |
| Author | Fujita, Hirofumi| Shiosaka, Masahiko| Ogino, Tetsuya| Okimura, Yuya| Utsumi, Toshihiko| Sato, Eisuke F.| Akagi, Reiko| Inoue, Masayasu| Utsumi, Kozo| Sasaki, Junzo| |
|---|---|
| Published Date | 2008-06-23 |
| Publication Title | Brain Research |
| Volume | volume1206 |
| Content Type | Journal Article |
