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Author Tomiyama, Yasuaki| Shinohara, Tsugumichi| Matsuka, Mirai| Bando, Tetsuya| Mito, Taro| Tomioka, Kenji|
Keywords Circadian clock Clockwork orange Clock gene Cricket cry2 Molecular oscillation Locomotor rhythm
Published Date 2020-11-11
Publication Title Zoological Letters
Volume volume6
Issue issue1
Publisher BMC
Start Page 12
ISSN 2056-306X
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s). 2020
File Version publisher
DOI 10.1186/s40851-020-00166-4
Web of Science KeyUT 000588683700001
Related Url isVersionOf https://doi.org/10.1186/s40851-020-00166-4
FullText URL fulltext.pdf
Author Habuta, Munenori| Yasue, Akihiro| Suzuki, Ken-Ichi T.| Fujita, Hirofumi| Sato, Keita| Kono, Hitomi| Takayama, Ayuko| Bando, Tetsuya| Miyaishi, Satoru| Oyadomari, Seiichi| Tanaka, Eiji| Ohuchi, Hideyo|
Published Date 2020-10-15
Publication Title PLoS ONE
Volume volume15
Issue issue10
Publisher Public Library of Science
Start Page e0240333
ISSN 1932-6203
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © 2020 Habuta et al.
File Version publisher
PubMed ID 33057360
DOI 10.1371/journal.pone.0240333
Web of Science KeyUT 000581814700082
Related Url isVersionOf https://doi.org/10.1371/journal.pone.0240333
JaLCDOI 10.18926/AMO/59950
FullText URL 74_3_199.pdf
Author Fujita, Hirofumi| Bando, Tetsuya| Oyadomari, Seiichi| Ochiai, Kazuhiko| Watanabe, Masami| Kumon, Hiromi| Ohuchi, Hideyo|
Abstract Dickkopf 3 (Dkk3) is a secreted protein belonging to the Dkk family and encoded by the orthologous gene of REIC. Dkk3/REIC is expressed by mouse and human adrenal glands, but the understanding of its roles in this organ is still limited. To determine the functions of Dkk3 in the mouse adrenal gland, we first identified that the mouse Dkk3 protein is N-glycosylated in the adrenal gland as well as in the brain. We performed proteome analysis on adrenal glands from Dkk3-null mice, in which exons 5 and 6 of the Dkk3 gene are deleted. Twodimensional polyacrylamide gel electrophoresis of adrenal proteins from wild-type and Dkk3-null mice revealed 5 protein spots whose intensities were altered between the 2 genotypes. Mass spectrometry analysis of these spots identified binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) chaperone. To determine whether mouse Dkk3 is involved in the unfolded protein response (UPR), we carried out a reporter assay using ER-stress responsive elements. Forced expression of Dkk3 resulted in the induction of distinct levels of reporter expression, showing the UPR initiated by the ER membrane proteins of activating transcription factor 6 (ATF6) and inositol-requring enzyme 1 (IRE1). Thus, it is possible that Dkk3 is a physiological ER stressor in the mouse adrenal gland.
Keywords Dkk3 knockout mouse adrenal gland glucose-regulated protein 78 proteome endoplasmic reticulum stress
Amo Type Original Article
Published Date 2020-06
Publication Title Acta Medica Okayama
Volume volume74
Issue issue3
Publisher Okayama University Medical School
Start Page 199
End Page 208
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2020 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 32577017
Web of Science KeyUT 000543363400003
NAID 120006862792
Author Hamada, Yoshimasa| Bando, Tetsuya| Nakamura, Taro| Ishimaru, Yoshiyasu| Mito, Taro| Noji, Sumihare| Tomioka, Kenji| Ohuchi, Hideyo|
Published Date 2015-09
Publication Title Development
Volume volume142
Issue issue17
Content Type Journal Article
Author Bando, Tetsuya|
Published Date 2015-10
Publication Title Okayama University Medical Research Updates
Volume volume16
Content Type Others