| Author | Miyake, Yoshiaki| Furumatsu, Takayuki| Kubota, Satoshi| Kawata, Kazumi| Ozaki, Toshifumi| Takigawa, Masaharu| |
|---|---|
| Published Date | 2011-06-03 |
| Publication Title | Biochemical and Biophysical Research Communications |
| Volume | volume409 |
| Issue | issue2 |
| Content Type | Journal Article |
| FullText URL | Fulltext.pdf fig.pdf table.pdf |
|---|---|
| Author | Matsumoto, Yohsuke| Motoki, Takahiro| Kubota, Satoshi| Takigawa, Masaharu| Tsubouchi, Hirohito| Gohda, Eiichi| |
| Keywords | hepatocyte growth factor valproic acid histone deacetylase inhibitor butyric acid trichostatin A induction tumor invasion dermal fibroblast |
| Note | Published with permission from the copyright holder. This is a author's copy,as published in Biochemical and Biophysical Research Communications, 2008 Vol.366 Issue.1 pp.110-116 | |
| Published Date | 2008-02-01 |
| Publication Title | Biochemical and Biophysical Research Communications |
| Volume | volume366 |
| Issue | issue1 |
| Publisher | Academic Press |
| Start Page | 110 |
| End Page | 116 |
| ISSN | 0006-291X |
| NCID | AA00564395 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 18053801 |
| DOI | 10.1016/j.bbrc.2007.11.089 |
| Web of Science KeyUT | 000252392400018 |
| Author | Moritani, Norifumi H| Kubota, Satoshi| Sugahara, Toshio| Takigawa, Masaharu| |
|---|---|
| Published Date | 2005-04-15 |
| Publication Title | Cell Communication and Signaling |
| Volume | volume3 |
| Issue | issue1 |
| Content Type | Journal Article |
| Author | Chujo, Sonoko| Shirasaki, Fumiaki| Kawara, Shigeru| Inagaki, Yutaka| Kinbara, Takuro| Inaoki, Makoto| Takigawa, Masaharu| Takehara, Kazuhiko| |
|---|---|
| Published Date | 2004-12 |
| Publication Title | Journal of Cellular Physiology |
| Volume | volume203 |
| Issue | issue2 |
| Content Type | Journal Article |
| Author | Asano, Masahiro| Kubota, Satoshi| Nakanishi, Tohru| Nishida, Takashi| Yamaai, Tomoichiro| Yosimichi, Gen| Ohyama, Kazumi| Sugimoto, Tomosada| Murayama, Yoji| Takigawa, Masaharu| |
|---|---|
| Published Date | 2005-10-05 |
| Publication Title | Cell Communication and Signaling |
| Volume | volume3 |
| Issue | issue1 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/30956 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Doi, Hideyuki| Nishida, Keiichiro| Yorimitsu, Masanori| Komiyama, Takamitsu| Kadota, Yasutaka| Tetsunaga, Tomonori| Yoshida, Aki| Kubota, Satoshi| Takigawa, Masaharu| Ozaki, Toshifumi| |
| Abstract | Mechanical stress plays a key role in the pathogenesis of cartilage destruction seen in osteoarthritis (OA). We investigated the effect of cyclic tensile stress (CTS) on the anabolic and catabolic gene expression of rat cultured normal chondrocytes using the Flexercell strain unit. The effects of interleukin (IL)-4, a chondroprotective cytokine, on the changes in gene expression induced by CTS were also investigated. CTS (7% elongation at 0.5 Hz) for 24 h did not affect the expression of aggrecan and type II collagen, whereas CTS significantly upregulated matrix metalloproteinase (MMP)-13 and cathepsin B mRNA expression by chondrocytes. IL-1beta expression was also signifi cantly upregulated by CTS up to 12 h. The upregulation of MMP-13 was observed at 3 h, which was earlier than that of IL-1beta. Furthermore, pre-treatment with IL-4 (10 ng/ml) suppressed both MMP-13 and cathepsin B induction by mechanical stress, as well as CTS-induced IL-1beta expression. Our results suggest that IL-4 might have a therapeutic value in the treatment of OA by downregulation of mechanical stress-induced MMP-13 and cathepsin B expression by chondrocytes. |
| Keywords | IL-4 MMP cathepsin B mechanical stress aggrecanase |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2008-04 |
| Volume | volume62 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 119 |
| End Page | 126 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 18464888 |
| Web of Science KeyUT | 000255297600008 |
| JaLCDOI | 10.18926/AMO/30739 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Aiga, Ayako| Asaumi, Koji| Lee, You Jin| Kadota, Hiroaki| Mitani, Shigeru| Ozaki, Toshifumi| Takigawa, Masaharu| |
| Abstract | The localization and expression of neurotrophins and their receptors during distraction osteogenesis was investigated in 72 male rat femurs (11 weeks old) to further clarify the concurrence of cellular and molecular events of new bone formation. After osteotomy, a 7-day lag phase was followed by distraction at the rate of 0.25 mm/12 h for 21 days (distraction phase), and a 7-day consolidation phase. The localization of neurotrophins (NGF, BDNF and NT-3) and their receptors tropomyosinrelated kinases (TRKA, TRKB and TRKC) by immunostaining showed positive staining in bone forming cells in each stage, although the presence and staining intensity varied by cell type and phase. The expressions of NGF, BDNF and NT-3 by real-time polymerase chain reaction (real-time PCR) showed that the peak of the mRNA expression of NGF occurred 10 days after distraction. NT-3 increased during bone extension, but decreased when distraction stopped. In contrast, BDNF continued to increase gradually throughout the distraction and consolidation phases. These findings suggest that neurotrophins and their receptors may play different roles in endochondral and intramembranous ossification in distraction osteogenesis. The tension stress caused by distraction may stimulate the expression of neurotrophins and their receptors, and promote osteogenesis. |
| Keywords | neurotrophin Trk distraction osteogenesis mechanical stress |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-10 |
| Volume | volume60 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 267 |
| End Page | 277 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 17072373 |
| Web of Science KeyUT | 000241509000003 |
