Acta Medica Okayama1559-23167122012Functional characterization of a novel plasma membrane intrinsic protein2 in barley18421846ENMineoShibasakaSizukaSasanoSigekoUtsugiMakiKatsuharaWater homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2;8 in barley (Hordeum vulgare). HvPIP2;8 shared 72–76% identity with other HvPIP2s and 74% identity with rice OsPIP2;8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2;8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2;8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1;2 was not observed. These results suggest that HvPIP2;8 plays a role in water homeostasis although further functional analysis is required in future.No potential conflict of interest relevant to this article was reported.岡山大学資源生物科学研究所Acta Medica Okayama0916-930X321995ルシフェリン―ルシフェラーゼ法によるアデニル酸定量のための大麦根からのサンプル抽出方法の検討137143ENYoshikoAkiyamaMineoShibasakaToshioKawasakiThree methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged.No potential conflict of interest relevant to this article was reported.岡山大学資源生物科学研究所Acta Medica Okayama0916-930X321995水耕栽培におけるオオムギの生育に及ぼすマンノースの影響129135ENMineoShibasakaMasahikoMiyataYoshikoAkiyamaToshioKawasakiTo examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants.No potential conflict of interest relevant to this article was reported.岡山大学資源生物科学研究所Acta Medica Okayama0916-930X221994イネ水中芽生えの酸素適応過程におけるチトクロムcと１１．９kDaタンパク質の増加149157ENMineoShibasakaTakashiUshimaruKatsuyukiOokuboShin-ichiTsuchidaHideoTsujiTo examine the changes in cytocrome c content in submerged rice seedlings after exposure to air, antiserum was prepared against purified cytocrome c from rice bran. Western blottong analysis revealed that cytochrome c was detected 6 h after exposure to air, but not detected in submerged rice seedling. On a fresh weight basis, the same level of cytochrome c as that of the aerobic control was found in the 24-h-air adapted seedlings. judging from the high A408/A280 ratio (4.66),the cytochrome c preparation used as antigen was considered to be well purified. However, the antiserum reacted other several polypeptides. One of them reacted more strongly against the antisermu than cytochrome c and its molecular weight was estimated as 11.9 kDa. The polypeptide increased during air adaptation and the levels found in both submerged seedlings and aerobic control were lower than that in 24-h-air-adapted seedlings.No potential conflict of interest relevant to this article was reported.岡山大学資源生物科学研究所Acta Medica Okayama0916-930X221994イネ水中芽生えの酸素適応過程におけるミトコンドリアスペクトルの変化Gaussian Deconvolutionによる解析135147ENMineoShibasakaHideoTsujiQuantitative changes in mitochondrial cytochromes of submerged rice seedlings after exposure to air were investigated using gaussian deconvolution analysis on absorption spectrum at liquid nitrogen temperature. Differnece spectra were obtained from subtracting the absorption spectra of oxidized mitochondria from reduced mitochondria by succinate with antimycin-A. they showed three kinds of b-type cytochrome and a spectral component similar to cytochrome c1. Subtraction of the cytochrome c1-like component from the spectrum by curve analysis showed that three cytochrome bs had similar absorption maximum and that on a mitochondrial protein basis they were constant during air-adaptation. Cytochrome c per mitochontorial protein in submerged seedlings was about half of that in aerobic seedlings and increased by 1.5-fold during air-adaptation. The change in cytochrome c content was in paralleled with that of cytochrome aa3. These findings revealed that there were two groups of cytochromes in the response to air-adaptation,i.e.,three cytochrome bs were constant and cytochoromes c and aa3 increased in parallel.No potential conflict of interest relevant to this article was reported.