start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=4 article-no= start-page=522 end-page=529 dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=2025 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of Intermittent Low-temperature Storage Duration and Cycle on the Bolting and Flowering of Delphinium elatum in Summer en-subtitle= kn-subtitle= en-abstract= kn-abstract=Early-bolting in summer is a major problem when growing delphinium seedlings in summer to produce cut flowers that will be shipped in autumn and winter. In this study, an intermittent low-temperature storage (ILTS) treatment that induces flower bud differentiation in strawberry and prevents rosette formation in Eustoma significantly increased the Delphinium elatum cut flower length. Moreover, ILTS was as effective as growing seedlings under cool conditions at preventing early-bolting. We analyzed the effects of six ILTS treatments that differed regarding the treatment temperature (5 and 10C) and treatment cycle (3 days/3 days, 6 days/6 days, and 12 days/12 days; ambient conditions/cool and dark). Cut flowers were significantly longer with the 6 days/6 days treatment at 10C than for the control treatment. Furthermore, repeating the ILTS treatment cycle (6 days ambient conditions/6 days at 10C) a total of four times produced high-quality cut flowers regardless of the cultivar. Therefore, this ILTS treatment may be ideal for preventing early-bolting in D. elatum. en-copyright= kn-copyright= en-aut-name=KawaiMika en-aut-sei=Kawai en-aut-mei=Mika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FukuyasuMiwa en-aut-sei=Fukuyasu en-aut-mei=Miwa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaYoshiyuki en-aut-sei=Tanaka en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KitamuraYoshikuni en-aut-sei=Kitamura en-aut-mei=Yoshikuni kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YasubaKen-ichiro en-aut-sei=Yasuba en-aut-mei=Ken-ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YoshidaYuichi en-aut-sei=Yoshida en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=GotoTanjuro en-aut-sei=Goto en-aut-mei=Tanjuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Agriculture, Kyoto University kn-affil= affil-num=4 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=5 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=6 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=7 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= en-keyword=cool storage kn-keyword=cool storage en-keyword=cut flower quality kn-keyword=cut flower quality en-keyword=high ambient temperature kn-keyword=high ambient temperature en-keyword=long day kn-keyword=long day en-keyword=Ranunculaceae kn-keyword=Ranunculaceae END start-ver=1.4 cd-journal=joma no-vol=93 cd-vols= no-issue=4 article-no= start-page=335 end-page=343 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=2024 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Elucidation of Low-temperature Regulated Flavone Synthesis in Dahlia Variabilis and its Effects on Flower Color en-subtitle= kn-subtitle= en-abstract= kn-abstract=Dahlia (Dahlia variabilis) flower colors are diverse and are determined by the accumulation of flavonoids. Cultivars with dark red flowers accumulate more anthocyanins in their petals. Flower color changes such as color fading often occur in some cultivars. In this study, low minimum temperature regulated flower color fading and flavonoid synthesis in dahlia eNesshof were investigated. The pigment contents and expression levels of flavonoid biosynthesis genes were investigated in detail under several growing environments in which color fading occurs. Flavones accumulate more in color-faded orange flowers than in dark red ray florets. The expression analysis of the anthocyanin synthesis pathway genes indicated that the upregulation of flavone synthase (DvFNS) gene expression correlated with the high accumulation of flavones in color-faded petals. DvFNS expression was also detected in young leaves, and the expression level was higher in winter than in summer. Seasonal changes in DvFNS expression in young leaves significantly correlated with color fading in petals. The change in DvFNS expression in young unexpanded leaves of relatively high-sensitive plants was significantly higher than that of low-sensitive plants before and after treatment under inductive conditions. In conclusion, low-temperature-inducible changes in the flavonoid accumulation in petals was suggested to reflect a change in DvFNS expression occurring in the meristem prior to flower bud formation. This temporal DvFNS expression in young unexpanded leaves of eNesshof dahlia could be an insight for the selection and breeding of non-color fading plants. en-copyright= kn-copyright= en-aut-name=K. MuthamiaEdna en-aut-sei=K. Muthamia en-aut-mei=Edna kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NaitoKoji en-aut-sei=Naito en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkadaHiromasa en-aut-sei=Okada en-aut-mei=Hiromasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KarasawaYukino en-aut-sei=Karasawa en-aut-mei=Yukino kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KikumuraTokuyu en-aut-sei=Kikumura en-aut-mei=Tokuyu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NaraTakuya en-aut-sei=Nara en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HamauzuYasunori en-aut-sei=Hamauzu en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MotokiKo en-aut-sei=Motoki en-aut-mei=Ko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YasubaKen-ichiro en-aut-sei=Yasuba en-aut-mei=Ken-ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YoshidaYuichi en-aut-sei=Yoshida en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KitamuraYoshikuni en-aut-sei=Kitamura en-aut-mei=Yoshikuni kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=GotoTanjuro en-aut-sei=Goto en-aut-mei=Tanjuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Faculty of Agriculture, Shinshu University kn-affil= affil-num=4 en-affil=Faculty of Agriculture, Shinshu University kn-affil= affil-num=5 en-affil=Faculty of Agriculture, Shinshu University kn-affil= affil-num=6 en-affil=Faculty of Agriculture, Shinshu University kn-affil= affil-num=7 en-affil=Faculty of Agriculture, Shinshu University kn-affil= affil-num=8 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=9 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=10 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=11 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=12 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=anthocyanin kn-keyword=anthocyanin en-keyword=dahlia kn-keyword=dahlia en-keyword=flavone synthase kn-keyword=flavone synthase en-keyword=seasonal color fading kn-keyword=seasonal color fading en-keyword=young unexpanded leaves kn-keyword=young unexpanded leaves END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=1 article-no= start-page=58 end-page=67 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220122 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fasciation in Strawberry Floral Organs and Possible Implications for Floral Transition en-subtitle= kn-subtitle= en-abstract= kn-abstract=Fasciation in strawberry is characterized by an enlarged and flattened receptacle, clustering of flowers, and altered inflorescence architecture. However, the developmental process of fasciated flowers remains obscure. In this study, the fasciation incidence and developmental process in the primary fruit and inflorescence architecture were evaluated and compared for the non-susceptible cultivars, eNyohof and eSagahonokaf and one of the most susceptible cultivars, eAi-Berryf. The severity and frequency of flower and inflorescence fasciation was clearly greater in the vigorously growing large plants of eAi-Berryf compared to small plants and large plants of the other two cultivars. In eAi-Berryf, the deformation of the large shoot apical meristem (SAM) into an oval shape was the initial symptom observed before and during floral transition. Such oval-shaped SAMs often differentiated two or more leaf primordia almost at the same time, which then developed into divided multiple vegetative SAMs before floral transition and linearly-fasciated SAMs during floral transition, respectively. The development of fasciation symptoms was observed after downregulation of FaTFL1. Although inflorescence or receptacle fasciation could be controlled when early and rapid floral induction was achieved by intermittent low-temperature treatment, severe fasciation was observed in late-flowered plants which were either not responsive or not subjected to this treatment. These results indicate that fasciation of floral organs may be triggered and develop during floral transition and that temperature fluctuations around boundary values between floral inhibition to induction may cause a half-finished or slowly processed floral transition and finally result in severe fasciation in vigorously growing eAi-Berryf plants. en-copyright= kn-copyright= en-aut-name=Thi CamNguyen en-aut-sei=Thi Cam en-aut-mei=Nguyen kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SunagawaNaomichi en-aut-sei=Sunagawa en-aut-mei=Naomichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SesumiMiho en-aut-sei=Sesumi en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KitamuraYoshikuni en-aut-sei=Kitamura en-aut-mei=Yoshikuni kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TanakaYoshiyuki en-aut-sei=Tanaka en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GotoTanjuro en-aut-sei=Goto en-aut-mei=Tanjuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YasubaKen-ichiro en-aut-sei=Yasuba en-aut-mei=Ken-ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=YoshidaYuichi en-aut-sei=Yoshida en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Sciences, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Sciences, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Environmental and Life Sciences, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Sciences, Okayama University kn-affil= affil-num=5 en-affil=Graduate School of Agriculture, Kyoto University kn-affil= affil-num=6 en-affil=Graduate School of Environmental and Life Sciences, Okayama University kn-affil= affil-num=7 en-affil=Graduate School of Environmental and Life Sciences, Okayama University kn-affil= affil-num=8 en-affil=Graduate School of Environmental and Life Sciences, Okayama University kn-affil= END start-ver=1.4 cd-journal=joma no-vol=110 cd-vols= no-issue= article-no= start-page=7 end-page=13 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies for stabilization of flower color and cut flower longevity kn-title=ԐF̈艻Ɛ؂Ԃ̉Ɋւ錤 en-subtitle= kn-subtitle= en-abstract= kn-abstract=@My research interest is in the physiology and ecology of ornamental plants. Here, I introduce my research subjects on the stabilization of flower color and cut flower longevity. Seasonal color change of dahlia (Dahlia variabilis) is studied as practical study for improving the stable dahlia cut flower production during autumn and winter. The results obtained from pigment analysis and clone selection are presented. Research on cut flower longevity is presented from two aspects. Firstly, study on the structure of conductive tissues that contribute to water transport is presented. Secondly, study on the floral organs that contribute to transpiration from cut flowers is presented. en-copyright= kn-copyright= en-aut-name=KitamuraYoshikuni en-aut-sei=Kitamura en-aut-mei=Yoshikuni kn-aut-name=kÖM kn-aut-sei=k kn-aut-mei=ÖM aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=Rww@‹Ȋw en-keyword=cut flower kn-keyword=cut flower en-keyword=flower color kn-keyword=flower color en-keyword=transpiration kn-keyword=transpiration END