start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=裏表紙・英文目次 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学農学部 kn-aut-sei=岡山大学農学部 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=奥付 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学農学部 kn-aut-sei=岡山大学農学部 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=45 end-page=59 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The List of Published by Members of the Faculty From January to December 2013. kn-title=公表学術論文等リスト 2013 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学農学部 kn-aut-sei=岡山大学農学部 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=37 end-page=43 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Research on capsiconinoid contents, nonpungent capsaicinoid analogues, in Capsicum cultivars kn-title=トウガラシにおける新規カプサイシン類似物質・カプシコニノイドの含量 en-subtitle= kn-subtitle= en-abstract= kn-abstract= In the course of analyses of nonpungent capsaicinoid analogs named capsinoids, two unknown compounds were discovered in pepper fruits. These compounds were isolated from the fruit of ‘CCB’(Capsicum baccatum var. praetermissum). Their structures were determined to be coniferyl (E)?8?methyl?6?nonenoate and coniferyl 8?methylnonanoate. These novel capsaicin analogs were named capsiconiate and dihydrocapsiconiate, respectively, and the coniferyl ester group was named capsiconinoid. Capsiconinoids have agonist activity for transient receptor potential vanilloid type 1, and their pungency is very low, as similar to that of capsinoids. Cultivars containing high levels of capsiconinoid are considered to be important for vegetable or dietary supplement. HPLC analysis was conducted to determine capsiconinoid content in fruits of 54 Capsicum cultivars : 28 cultivars of C. annuum, 9 of C. baccatum, 12 of C. chinense, 4 of C. frutescens, and 1 of C. pubescens. Twelve cultivars contained capsiconinoids. ‘CCB’ showed the highest capsiconinoid content level (3314 μg・g?1 DW) and ‘Charapita’ (C. chinense) had the second highest (2694 μg・g?1 DW). The other 10 cultivars contained much lower capsiconinoid than these two cultivars (<300 μg・g?1 DW). Time-course analysis during fruit development clarified that capsiconinoid content increased and reached maximum level in green mature fruit. Capsaicinoid contents also increased, correspondingly. As fruits matured, capsiconinoid contents decreased rapidly, while capsaicinoid content either did not change or decreased slightly. en-copyright= kn-copyright= en-aut-name=TanakaYoshiyuki en-aut-sei=Tanaka en-aut-mei=Yoshiyuki kn-aut-name=田中義行 kn-aut-sei=田中 kn-aut-mei=義行 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 en-keyword=pepper kn-keyword=pepper en-keyword=capsaicinoid analogs kn-keyword=capsaicinoid analogs en-keyword=capsiconinoid kn-keyword=capsiconinoid END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=31 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation and characterization of plant immune-priming chemicals kn-title=植物免疫プライミング剤の単離と作用機序解明 en-subtitle= kn-subtitle= en-abstract= kn-abstract= Plant disease resistance inducers, so-called plant activators, are agrochemicals that protect crops from pathogens. They confer long-lasting resistance against a broad range of diseases by activating their immune system. Since plant activators impinge on host plants, unlike commonly-used pesticides which directly target pathogens, no drug-resistant microbes for plant activators have been found so far in the field. They originated from probenazole (Oryzemate ) and have been widely used over 30 years for the protection of paddy-field rice from blast fungus and bacterial leaf blight in East Asia. In spite of the advantages of plant activators, their application is still limited. The lack of both knowledge about their modes of action and an appropriate high-throughput screening system restrict the isolation of novel compounds. We established a highthroughput chemical screening procedure to identify plant immune-priming compounds which increase but do not directly induce immune responses in Arabidopsis suspension cells upon infection of the bacterial pathogen, Pseudomonas syringae pv. tomato DC3000 avrRpm1. From the screening of a commercially available chemical library of 10,000 diverse small organic molecules, we identified seven compounds that prime the immune response and we designated them ‘imprimatins’ for immune-priming chemicals. The isolated compound, imprimatin C1 activates the expression of defense-related genes independent of pathogen and functions as a weak analog of salicylic acid (SA). Those originally-isolated three compounds and their four derivatives were classified into two groups with distinct molecular structures and they weve named imprimatins A and B. We found that they conferred disease resistance in plants by inhibiting both a known and a previously unknown SA glucosyltransferase (SAGT). Imprimatins and their targets are useful for the development of practical plant activators and also their modes of action might give a clue for novel crop protection technology. en-copyright= kn-copyright= en-aut-name=NoutoshiYoshiteru en-aut-sei=Noutoshi en-aut-mei=Yoshiteru kn-aut-name=能年義輝 kn-aut-sei=能年 kn-aut-mei=義輝 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 en-keyword=plant disease resistance kn-keyword=plant disease resistance en-keyword=plant immunity kn-keyword=plant immunity en-keyword=disease resistance inducer kn-keyword=disease resistance inducer en-keyword=plant activator kn-keyword=plant activator en-keyword=chemical biology kn-keyword=chemical biology END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=21 end-page=30 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=コムギ染色体欠損系統を用いた新規活性型レトロトランスポゾン TriRe-1 の分子遺伝学的解析 kn-title=Characterization of a novel retrotransposon TriRe?1 using nullisomic-tetrasomic lines of hexaploid wheat en-subtitle= kn-subtitle= en-abstract= レトロトランスポゾンは植物ゲノムの主要な構成要素であり,コムギゲノムにおいてはその80オを占める.特に LTR 型レトロトランスポゾンの割合が高く,ゲノムの拡大,配列の多様性およびゲノム構造変異等に大きく寄与し てきたと考えられている.これら配列は自身のコピー配列を複製し増幅するため,ゲノム中には数百,数千に及ぶコ ピー配列をもつ.また,ゲノム進化の過程において多数のファミリーを形成してきた.これら多数のファミリーのう ち,現在でも転移活性を示す活性型ファミリーは,品種間において高い挿入多型を示すことが知られている.このよ うな挿入多型は,連鎖解析および系統解析等各種遺伝解析に利用可能である. 本研究では,コムギにおける新規活性 型レトロトランスポゾンファミリー TriRe-1 の特徴を詳細に解析した.TriRe-1 は転移に必要なタンパク質をコー ドする内部配列をもち,また日本で育成されたコムギ近縁品種間においても高い挿入多型を示したため,現在でも転 移活性を有している,もしくはごく最近まで転移していた可能性が高いと考えられた.一方で,コムギ染色体欠損系 統(ナリソミックテトラソミック系統)を用い,TriRe-1 の挿入箇所を比較解析した.その結果,大部分の挿入箇所 は複数の同祖染色体に存在すると考えられたが,Bゲノムにおいて最も多くの特異的な挿入箇所が同定された.よっ て,Bゲノム祖先種において活発に増幅してきた可能性が示唆された.今回の結果により,新規活性型レトロトラン スポゾン TriRe-1 の品種間挿入多型を利用した DNA マーカー,また,各ゲノム(A,B,Dゲノム)特異的な挿 入箇所を利用したゲノム識別性に優れた DNA マーカーの開発の可能性が期待される. kn-abstract= Retrotransposons constitute the large fraction (〜80%) of the wheat genome where numerous and diverse retrotransposon families exist, where especially the long terminal repeat (LTR) retrotransposon family is known to be predominant. Thus, they have been considered to contribute to the genome expansion, sequence diversification and the genome structure alternation in the wheat genome. In addition, the insertion polymorphism of the LTR retrotransposon family among the cultivars has been known to be quite useful for the genetic analysis such as the linkage mapping and the phylogenetic studies. Here, we report the characteristics of a novel active LTR retrotransposon family TriRe?1, which belongs to the Ty1?copia group in the hexaploid wheat (Triticum aestivum L.) genome. This retroelement appears to encode all proteins required for the transposition and showed high insertion polymorphism among the hexaploid wheat cultivars, suggesting its potential of transpositional activity with at least recent transposition during wheat evolution. We studied the chromosomal localization of the TriRe?1 insertion site based on the genome-wide comparative analysis using the nullisomic-tetrasomic lines of the cultivar Chinese Spring. The results showed that although the majority of the TriRe?1 insertion sites exist across the homoeologous chromosomes of A, B or D genomes, a higher number of insertions in the B genome was detected compared to A or D genome, suggesting a specific amplification in the history of B genome progenitors. In conclusion, a novel LTR retrotransposon TriRe?1 should be valuable for the development of molecular markers based on insertion polymorphism among the cultivars, and also the genome-specific TriRe?1 insertion site can be utilized to study evolutional history of wheat genomes. en-copyright= kn-copyright= en-aut-name=MondenYuki en-aut-sei=Monden en-aut-mei=Yuki kn-aut-name=門田有希 kn-aut-sei=門田 kn-aut-mei=有希 aut-affil-num=1 ORCID= en-aut-name=TakaiTakeru en-aut-sei=Takai en-aut-mei=Takeru kn-aut-name=高井健 kn-aut-sei=高井 kn-aut-mei=健 aut-affil-num=2 ORCID= en-aut-name=TaharaMakoto en-aut-sei=Tahara en-aut-mei=Makoto kn-aut-name=田原誠 kn-aut-sei=田原 kn-aut-mei=誠 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Retrotransposon kn-keyword=Retrotransposon en-keyword=Wheat kn-keyword=Wheat en-keyword=Molecular markers kn-keyword=Molecular markers en-keyword=Nullisomic-tetrasomic lines kn-keyword=Nullisomic-tetrasomic lines END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=11 end-page=19 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of potassium nutrition on fruit development and yield of substrate grown strawberry kn-title=養液栽培イチゴの生育・収量と果実発育に及ぼすカリウム栄養の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= Effect of K nutrition on sugar and organic acid concentration in fruit and yield of strawberry cultivars (Fragaria ×ananassa Duch.; Nyoho, Toyonoka, Sachinoka, Asukarubi, Akihime, Tochiotome, and Sagahonoka) was investigated. Nutrient solution (NO3 8, NH4 1, P 1, K 4, Ca 2, Mg 1, SO4 1 ; mM) was modified to contain 0 to 4mM of K by replacing K with Ca and supplied from the beginning of flowering. K in the drainage decreased to a trace level 3 weeks after the beginning of treatment except for 4mM?K solution. Absorption of NO3 apparently decreased in plants supplied 0mM?K solution, but little difference was observed among the other 3 solutions. K concentration in petiole decreased linearly with decrease in K concentration in solutions, but there was little difference between the concentrations of leaflet of plants supplied with 4 and 2mM?K solutions. No difference was observed in concentrations of sugars and organic acids in fruit in primary inflorescence. In the second inflorescence, organic acids and K concentration in fruit linearly decreased with decrease in K in supplied solutions while sugar concentration of fruit decreased significantly only in plants supplied 0mM?K solution. When 0.5 to 4mM?K solutions were supplied from 2 weeks after planting, marketable fruit yield was smallest in plants supplied 0.5mM?K solution followed, by that supplied 1mM?K solution, and largest in that supplied with 2mM?K solution. Almost all K supplied with 2mM?K solution was absorbed by strawberry plants and no difference was observed in quality and K concentration of fruit, yield and plant growth between the plants supplied with 2 and 4mM?K solutions. It may be suitable to reduce the concentration of K in nutrient solution by half for substrate production of strawberry. en-copyright= kn-copyright= en-aut-name=YoshidaYuichi en-aut-sei=Yoshida en-aut-mei=Yuichi kn-aut-name=吉田裕一 kn-aut-sei=吉田 kn-aut-mei=裕一 aut-affil-num=1 ORCID= en-aut-name=OhmoriToshimasa en-aut-sei=Ohmori en-aut-mei=Toshimasa kn-aut-name=大森敏正 kn-aut-sei=大森 kn-aut-mei=敏正 aut-affil-num=2 ORCID= en-aut-name=GotoTanjuro en-aut-sei=Goto en-aut-mei=Tanjuro kn-aut-name=後藤丹十郎 kn-aut-sei=後藤 kn-aut-mei=丹十郎 aut-affil-num=3 ORCID= en-aut-name=TanakaYoshiyuki en-aut-sei=Tanaka en-aut-mei=Yoshiyuki kn-aut-name=田中義行 kn-aut-sei=田中 kn-aut-mei=義行 aut-affil-num=4 ORCID= en-aut-name=MurakamiKenji en-aut-sei=Murakami en-aut-mei=Kenji kn-aut-name=村上賢治 kn-aut-sei=村上 kn-aut-mei=賢治 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=農林水産省中国四国農政局 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=石川県立大学生物資源環境学部 en-keyword=Fragaria ×ananassa Duch. kn-keyword=Fragaria ×ananassa Duch. en-keyword=K/Ca ratio kn-keyword=K/Ca ratio en-keyword=organic acids kn-keyword=organic acids en-keyword=sugars kn-keyword=sugars END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=5 end-page=9 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Characterization of L-Arginine Oxidase Made from L-Glutamate Oxidase kn-title=高基質特異性L-グルタミン酸オキシダーゼより作成した基質特異性改変酵素L-アルギニンオキシダーゼの性質検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract= L?Glutamate oxidase (LGOX) from Streptomyces sp. X?119?6 has strict substrate specificity toward L?glutamate. Recently, we solved the X?ray crystal structure of LGOX and this revealed that Arg305 in the active site is the key residue involved in substrate recognition. Therefore, we created 19 mutant enzymes of R305X?LGOX by saturation mutagenesis. One of them R305D?LGOX, Arg305 substituted with Asp exhibited oxidase activity for L?Arg. Optimum pH of R305D?LGOX mutant enzyme was pH 8.5. Interestingly, the activity of R305D?LGOX toward L?Arg was inhibited by phosphate. And furthermore, the substrate specificity of R305D?LGOX was affected by using buffer. The results of inhibition analysis suggest, that phosphate is a competitive inhibitor of R305D?LGOX when L?Arg is used as substrate. Kinetic analysis of R305D?LGOX showed that Km value and kcat value of R305D?LGOX toward l-Arg were 0.68 mM and 6.7 s-1 respectively. In this study, we showed that R305D?LGOX mutant enzyme is a novel l-arginine oxidase and useful for l-arginine biosensor. en-copyright= kn-copyright= en-aut-name=NakaiRyuichiro en-aut-sei=Nakai en-aut-mei=Ryuichiro kn-aut-name=中井隆一郎 kn-aut-sei=中井 kn-aut-mei=隆一郎 aut-affil-num=1 ORCID= en-aut-name=FujinoShihoko en-aut-sei=Fujino en-aut-mei=Shihoko kn-aut-name=藤野志保子 kn-aut-sei=藤野 kn-aut-mei=志保子 aut-affil-num=2 ORCID= en-aut-name=UtsumiTomohiro en-aut-sei=Utsumi en-aut-mei=Tomohiro kn-aut-name=内海友宏 kn-aut-sei=内海 kn-aut-mei=友宏 aut-affil-num=3 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=4 ORCID= en-aut-name=KusakabeaHitoshi en-aut-sei=Kusakabea en-aut-mei=Hitoshi kn-aut-name=日下部均 kn-aut-sei=日下部 kn-aut-mei=均 aut-affil-num=5 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=�潟Gンザイムセンサ affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=L-glutamate oxidase kn-keyword=L-glutamate oxidase en-keyword=L-arginine oxidase kn-keyword=L-arginine oxidase en-keyword=biosensor kn-keyword=biosensor en-keyword=modified substrate specificity kn-keyword=modified substrate specificity en-keyword=L-amino acid oxidase kn-keyword=L-amino acid oxidase END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=大豆発芽時期におけるグリシニン分解酵素の活性変動 kn-title=Changes in Glycinin?Digesting Protease Activity During Soybean Germination. en-subtitle= kn-subtitle= en-abstract= 大豆発芽期におけるグリシニン分解酵素 (98 kDa SBP) の活性変動を解析した.大豆種子を4時間水で膨潤後, 25 ℃ 暗黒下で発芽させた.経時的にサンプリングを行い,2M NaCl を含むトリス緩衝液 (? 7.0) により粗酵素を抽出 後,グリシニン由来のトリプシン分解ペプチドを基質としてグリシニン分解酵素の活性変動を逆相 HPLC により追 跡した.その結果,種子膨潤後4日間比活性はほぼ一定の値を保ち,以後徐々に低下することが分かった.次いで, 粗酵素溶液からイオン交換 HPLC により98 kDa SBP を部分精製するとともに,発芽期における 98 kDa SBP の消長 を解析したところ,98 kDa SBP は乾燥種子及び各発芽段階の種子中全てに認められ,かつグリシン分解活性もグリ シニン由来のトリプシン分解ペプチド基質に対する活性と同様に認められた.以上の結果から,98 kDa SBP は種子 発芽に伴い誘導されるプロテアーゼではなく,種子貯蔵型のプロテアーゼであることが明らかになった. kn-abstract= Changes in glycinin-digesting protease activity during soybean germination have been investigated. The glycinin-digesting protease activities of imbibed or germinated soybean seed were assayed by RP?HPLC using a tryptic peptide from CM?glycinin or by SDS?PAGE using CM?glycinin as the endogenous substrate. Proteolytic activities of the germinated soybean seeds were found through the whole period of germination, the activities were maintained significantly unchanged during germination for 4 days, and then those specific activities declined slowly. AE?HPLC analysis of the glycinin-digesting protease in the imbibed or germinated soybean seeds showed unchanged peaks corresponding to glycinin- digesting activity, suggesting that the glycinin-digesting protease was not induced during germination but had already been synthesized during seed maturation. en-copyright= kn-copyright= en-aut-name=Md. Akhtaruzzaman en-aut-sei=Md. Akhtaruzzaman en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name=前田恵 kn-aut-sei=前田 kn-aut-mei=恵 aut-affil-num=2 ORCID= en-aut-name=KitagawaKeiko en-aut-sei=Kitagawa en-aut-mei=Keiko kn-aut-name=北川恵子 kn-aut-sei=北川 kn-aut-mei=恵子 aut-affil-num=3 ORCID= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=4 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Plant protease kn-keyword=Plant protease en-keyword=glycinin kn-keyword=glycinin en-keyword=germination kn-keyword=germination en-keyword=Glycine max kn-keyword=Glycine max END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=目次 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学農学部 kn-aut-sei=岡山大学農学部 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=表紙 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学農学部 kn-aut-sei=岡山大学農学部 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END