| ID | 16309 |
| Eprint ID | 16309
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| FullText URL | |
| Title Alternative | Studies on galactosyl transferase activity of gastric mucous in rats Part 1 Studies on an assay method and changes in gastric mucosal UDP-galactosyl transferase activity during fasting
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| Author |
Nagahara, Akimasa
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| Abstract | A method to measure the activity of an enzyme catalyzing sugar chains which are abundant in O-glycosidic linkage was studied using UDP-galactosyl transferase (UDP-Gal-T) found in the gastric mucosa of rat and asialo-bovine-submaxillar-mucin (ASBSM) as a substrate. The optimum pH for the measurement was 7.5, Mn(++) was required, and about twice as much Triton X-100 was needed as when asialo-agalacto-fetuin (ASGF) was used as the substrate. The activity of UDP-Gal-T was measured simultaneously in a group of rats receiving food and a group fasted for 24 hours, using ASGF and ASBSM as substrates. With ASGF no significant difference was seen in either the glandular corpus or the pylorus, while with ASBSM, the activity decreased significantly (p<0.05) in the glandular corpus, though no significant difference was seen in the pylorus.
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| Keywords | UDP-galactosyl transferase
asialo-agalacto-fetuin
asialo-bovine-submaxillar-mucin
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| Published Date | 1986-06-30
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| Publication Title |
岡山医学会雑誌
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| Publication Title Alternative | Journal of Okayama Medical Association
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| Volume | volume98
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| Issue | issue7-8
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| Publisher | 岡山医学会
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| Publisher Alternative | Okayama Medical Association
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| Start Page | 627
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| End Page | 633
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| ISSN | 0030-1558
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| NCID | AN00032489
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| Content Type |
Journal Article
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| Official Url | https://www.jstage.jst.go.jp/article/joma1947/98/7-8/98_7-8_627/_article/-char/ja/
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| Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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| language |
Japanese
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| Copyright Holders | 岡山医学会
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| File Version | publisher
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| Refereed |
True
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| Eprints Journal Name | joma
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