| ID | 16162 |
| Eprint ID | 16162
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| FullText URL | |
| Title Alternative | Molecular cloning of the SV40 small t antigen gene and purification of the antigen
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| Author |
Ikeda, Shogo
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| Abstract | The simian virus 40 (SV40) DNA fragment encoding small t antigen was cloned with expression vector pUC8 for the purification of small t-antigen. Small t antigen produced in Escherichia coli was a hybrid protein of 22,000 daltons which comprised about 6% of the total protein. Hybrid small t antigen maintained antigenic activity which was examined by Western-blotting and enzyme-linked immunoassay. Hybrid small t antigen was extracted from E. coli with urea and purified from a small t-antigen band in SDS-polyacrylamide gel. The purified antigen maintained antigenic activity.
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| Keywords | SV40 t 抗原
遺伝子のクローン化
発現ベクター
ウエスタン・ブロッティング
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| Published Date | 1985-06-30
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| Publication Title |
岡山医学会雑誌
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| Publication Title Alternative | Journal of Okayama Medical Association
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| Volume | volume97
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| Issue | issue5-6
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| Publisher | 岡山医学会
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| Publisher Alternative | Okayama Medical Association
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| Start Page | 419
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| End Page | 428
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| ISSN | 0030-1558
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| NCID | AN00032489
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| Content Type |
Journal Article
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| Official Url | https://www.jstage.jst.go.jp/article/joma1947/97/5-6/97_5-6_419/_article/-char/ja/
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| Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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| language |
Japanese
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| Copyright Holders | 岡山医学会
|
| File Version | publisher
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| Refereed |
True
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| Eprints Journal Name | joma
|
