start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=951
end-page=965
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Evaluation of Levamisole as Immunomodulator in Advanced Gastric Cancer
kn-title=Levamisoleを用いた進行胃癌免疫化学療法の臨床効果
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The efficacy of Levamisole (LMS) was studied on prolongation of patient survival and modulation of cellular immunity in a total of 220 cases of gastric cancer (136 cases for control and 84 cases for LMS group). In the study, detailed analyses were performed in 64 cases of stage Ⅲ, in which LMS therapy was effective and influence of background factors was minimum (32 cases for control and 32 cases for LMS group). LMS was administered 150mg/day for three consecutive days every other week beginning from three days before operation, and was continued more than than two months and upto two years. The results obtained were as follows: 1) In cases of gastric cancer, the survival rate of the LMS group, was significantly higher than that of the control group (p<0.05). Furthermore, the efficacy of LMS on prolongation of survival rate was remarkable in the group of stage Ⅲ (p<0.05). 2) A detailed study in the group of stage Ⅲ was performed and resulted in the following: First, when stage Ⅲ cases were classified into curative and noncurative cases, LMS was effective in prolonging the survival of patients who received curative resection. Secondly, the efficacy of LMS was investigated in terms of tumor size. No difference in patient survival was observed between the control and LMS group, when tumor size was less than 3.9cm or more than 8.0cm in diameter. However, a significant effect of LMS therapy was detected for cases whose tumor was between 4.0cm and 7.9cm in diameter and depth of invasion was se (P<0.05). Thirdly, influence of lymph node metastasis on the effect of LMS was analysed. LMS was effective to a significant degree if the metastasis did not extend beyond lymph nodes classified in group I (n(1)) (p<0.05). Fourthly, when localization or histopathological grading of tumor was considered, the survival rate of LMS group tended to be high if the tumor was localized in the bulk of the corpus and belonged to a undifferentiated type. Fifthly, the (v) factor, grade of invasion into veins, was evaluated. LMS was effective in cases of v(0). And also, the efficacy of LMS was observed in cases classified as INF β in terms of form of infiltration. 3) Mitogen responses, cell number and T-cell percentage of peripheral blood lymphocytes and also PPD skin test were examined to evaluate immunological status of gastric cancer patients receiving LMS therapy. The data suggested that LMS activated promptly and normalized the cellular immunity in cases of advanced gastric cancer classified into stage Ⅲ and Ⅳ in which cellular immunity was reduced. By contrast, it was ineffective for cases of gastric cancer at early stages classified into stage Ⅰ and Ⅱ.
en-copyright=
kn-copyright=

en-aut-name=OnoFumio
en-aut-sei=Ono
en-aut-mei=Fumio
kn-aut-name=小野二三雄
kn-aut-sei=小野
kn-aut-mei=二三雄
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=Levamisole
kn-keyword=Levamisole
en-keyword=胃癌
kn-keyword=胃癌
en-keyword=生存率
kn-keyword=生存率
en-keyword=細胞性免疫能
kn-keyword=細胞性免疫能
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=943
end-page=950
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Membrane lipids of Mycoplasma orale
kn-title=Mycoplasma oraleの膜脂質について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Lipid compositions of cell membrane of Mycoplasma orale CH 19299 were examined. The membrane lipids consisted of neutral lipids (43.5%) and phospholipids (56.5%). In the neutral lipid, 64% was cholesterol, 9% was cholesterol ester and the rest mainly consisted of triglycerides. Phospholipids comprize 53% of sphingomyelin, 32% of phosphatidylglycerol and 15% of phosphatidylcholine. There was not any lysophosphatidic acid, cardiolipin or glycosyldiglycerides. The fatty acids were restricted to C(16) and C(18) regardless of the phospholipid species. Phosphatidylglycerol was the sole phospholipid that was de novo synthesized in the cell. No fatty acid was synthesized when examined by using (14)C-acetate as the substrate. The mode of lipid synthesis in the cell membrane was discussed with reference to other mycoplasma species.
en-copyright=
kn-copyright=

en-aut-name=KukidaShozo
en-aut-sei=Kukida
en-aut-mei=Shozo
kn-aut-name=茎田祥三
kn-aut-sei=茎田
kn-aut-mei=祥三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部細菌学教室
en-keyword=Mycoplasma orale
kn-keyword=Mycoplasma orale
en-keyword=membrane lipidphos
kn-keyword=membrane lipidphos
en-keyword=phospholipid synthesis
kn-keyword=phospholipid synthesis
en-keyword=Fatty acid synthesis
kn-keyword=Fatty acid synthesis
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=931
end-page=942
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Myocardial protection by different cold cardioplegic solutions
kn-title=心保護灌流液の組成と心筋保護効果(心筋代謝と蘇生率よりみた保護効果)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Efficacy of three different solutions for myocardial protection was determined in dogs after 120 minute ischemia. In group I cold cardioplegia and topical hypothermia were performed with Ringer's solution in 9 dogs. Cold Ringer's solution, which was modified to contain potassium at 30 mEq/1 and sorbitol at 401 mOsm/1, was used in group Ⅱ (6 dogs). In 11 dogs (group Ⅲ) Collins solution (K: 115 mEq/1, 467 mOsm) was employed. The parameters measured included myocardial cyclic AMP, myocardial adenocine triphosphate, blood lactate, blood pyrvate, blood glucose, blood NEFA and O2 consumption. Results obtained were as follows: 1. Resuscitation rate was highest in group Ⅱ, being 100%, and lowest in group I, 11%. It was 45% in group Ⅲ. 2. Myocardial adenosine triphosphate decreased least in group Ⅱ (3.83μmol/g) and most in group I (2.18μmol/g). 3. There were no statistically significant inter-group differences in the other parameters. It was concluded that the best solution for myocardial protection was the modified Ringer's solution in group Ⅱ because the resuscitation rate was the best of the 3 groups. It was also noted that the resuscitation rate coincided well with the concentration of ATP in the myocardium. The Collins solution was suitable for stopping cardiac action, but not suitable for coronary perfusate because of a high potassium concentration which injured the myocardium. Cold Ringer's solution was effective in myocardial protection only by cooling the heart, not by its own protective action.
en-copyright=
kn-copyright=

en-aut-name=FujisawaReisuke
en-aut-sei=Fujisawa
en-aut-mei=Reisuke
kn-aut-name=藤澤禮介
kn-aut-sei=藤澤
kn-aut-mei=禮介
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二外科教室
en-keyword=心筋保護法
kn-keyword=心筋保護法
en-keyword=cyclic AMP
kn-keyword=cyclic AMP
en-keyword=ATP
kn-keyword=ATP
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=921
end-page=930
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=198210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Analysis of DNA replication of SV40 chromatin in digitonin-treated permeable cells
kn-title=ジギトニン処理透過性細胞内におけるSV40クロマチンDNA複製の解析
en-subtitle=
kn-subtitle=
en-abstract=The ultrastructure of digitonin-treated permeable cells and various conditions for DNA replication of SV40 chromatin as well as the requirements for and inhibitors of DNA synthesis were analyzed. Electron microscopy of the permeable cells revealed that the plasma membrane was injured but inner membranes and nuclear chromatin remained relatively intact. Efficient DNA replication of SV40 chromatin in the present system requires the addition of dATP, dCTP, dGTP, dTTP, ATP and Mg(2+). Complete replication of SV40 chromatin DNA occurs without addition of cytosol. The DNA synthesis is almost completely inhibited by ethylenediamin-tetraacetic acid, N-ethylmaleimide, aphidicolin, and arabinosyl-cytidine triphosphate but not by 2', 3'-dideoxythymidine -5'-triphosphate, showing that the DNA synthesis is replicative. Adriamycin strongly inhibits the DNA replication, and the addition of adriamycin in the process of reaction immediately stops the DNA replication. Thus, the digitonin-treated permeable cell system serves as a useful system for studying in vitro replication of chromatin DNA in eukaryotic cells as well as for studying the in vitro action of adriamycin and other antitumor drugs which inhibit DNA replication.
kn-abstract=真核細胞におけるクロマチンDNAの複製とその調節機構を研究するために, SV40感染細胞を低濃度のジギトニン処理し,基質透過性とした細胞(permeable cell)の超微構造と同細胞内におけるSV40クロマチンDNA複製系の諸条件を検討し,基質要求性と阻害剤や制癌剤の影響を解析した.その結果, ①至適なジギトニン濃度(100～150μg/ml),処理温度(25℃),塩濃度(50～100μM KCl),蔗糖濃度(0.1～0.15M), Mg(2+)濃度(5～7mM),反応温度(30°～37℃)などの基礎的諸条件を決定した. ②このジギトニン処理透過性細胞は超微構造的に細胞膜は障害されているが,他の内膜系や核クロマチンは比較的よく保たれている. ③この透過性細胞系では,細胞質抽出液を加えなくても,基質としての4種のデオキシリボヌクレオシド三リン酸(d ATP, d CTP, d GTP,各0.1mM, dTTP, 0.01mM)の他, ATP(2～5mM)とMg(2+) (5mM)の添加で効率のよいDNA合成が行なわれる. ④EDTA, NEM,アフィディコリン, ara CTPでDNA合成がほとんど完全に阻害され, dd TTPでは全く阻害されないので,この系のDNA合成は複製的DNA合成である. ⑤アドリアマイシンなどの制癌剤でSV40 DNAの複製が直ちに強く阻害されることを見出した.したがって,本実験系は真核細胞におけるクロマチンDNAのin vitro複製の研究のみならず,アドリアマイシンなどのDNA合成阻害制癌剤の作用を調べる上に極めて有用な実験系として役立つものと考えられる.
en-copyright=
kn-copyright=

en-aut-name=HanakawaShiro
en-aut-sei=Hanakawa
en-aut-mei=Shiro
kn-aut-name=花川志郎
kn-aut-sei=花川
kn-aut-mei=志郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部整形外科教室
en-keyword=ジギトニン
kn-keyword=ジギトニン
en-keyword=透過性細胞
kn-keyword=透過性細胞
en-keyword=SV40
kn-keyword=SV40
en-keyword=クロマチン
kn-keyword=クロマチン
en-keyword=DNA複製
kn-keyword=DNA複製
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=915
end-page=920
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Correlation between QT interval and RR interval on the epicardial electrogram in open-chest dog.
kn-title=心表面心電図でのQT intervalについて(実験的研究)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Correlation between QT interval and RR interval on epicardial electrogram in anesthetized open-chest dog was studied. The aortic blood pressure was kept above 70mmHg during the course of the experiment. RR interval was changed by left atrial pacing in the range of 0.243-0.870sec (0.412±0.119, mean±SD). In association with the change in RR interval, QT interval varied in the range of 0.190-0.360sec (0.253±0.028). The relation of QT interval to RR interval was statistically significant (r=0.744, p<0.01), though QT interval varied widely when the heart rate was under 100beat/min. QT interval correlated more closely to √RR interval than RR interval. The correlation equation was as follow: QT interval(sec)=0.24√RR interval (sec)+0.102, r=0.760, p<0.01. The data suggest that QT interval obtained by epicardial electrogram would be of use as an index of local electrical systole.
en-copyright=
kn-copyright=

en-aut-name=YasuharaKoichiro
en-aut-sei=Yasuhara
en-aut-mei=Koichiro
kn-aut-name=安原耕一郎
kn-aut-sei=安原
kn-aut-mei=耕一郎
aut-affil-num=1
ORCID=

en-aut-name=SaitoDaiji
en-aut-sei=Saito
en-aut-mei=Daiji
kn-aut-name=斉藤大治
kn-aut-sei=斉藤
kn-aut-mei=大治
aut-affil-num=2
ORCID=

en-aut-name=TakedaKou
en-aut-sei=Takeda
en-aut-mei=Kou
kn-aut-name=武田光
kn-aut-sei=武田
kn-aut-mei=光
aut-affil-num=3
ORCID=

en-aut-name=HyodoTazuo
en-aut-sei=Hyodo
en-aut-mei=Tazuo
kn-aut-name=兵頭多津男
kn-aut-sei=兵頭
kn-aut-mei=多津男
aut-affil-num=4
ORCID=

en-aut-name=HaraokaShoichi
en-aut-sei=Haraoka
en-aut-mei=Shoichi
kn-aut-name=原岡昭一
kn-aut-sei=原岡
kn-aut-mei=昭一
aut-affil-num=5
ORCID=

en-aut-name=NagashimaHideo
en-aut-sei=Nagashima
en-aut-mei=Hideo
kn-aut-name=長島秀夫
kn-aut-sei=長島
kn-aut-mei=秀夫
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部第一内科教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部第一内科教室

affil-num=4
en-affil=
kn-affil=岡山大学医学部第一内科教室

affil-num=5
en-affil=
kn-affil=岡山大学医学部第一内科教室

affil-num=6
en-affil=
kn-affil=岡山大学医学部第一内科教室
en-keyword=ＱＴ interval
kn-keyword=ＱＴ interval
en-keyword=RR interval
kn-keyword=RR interval
en-keyword=epicardial electrogram
kn-keyword=epicardial electrogram
en-keyword=anesthetized open-chest dog
kn-keyword=anesthetized open-chest dog
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=899
end-page=913
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies of gamma-aminobutyric acid (GABA) and its metabolite on the control mechanism of secretion of anterior pituitary hormones. Part Ⅱ: Effects of gamma-hydroxybutyric acid (GHB) on secretion of anterior pituitary hormones in human subjects.
kn-title=Gamma-aminobutyric acid (GABA) 及びその代謝産物の下垂体前葉ホルモン分泌調節における役割に関する研究 第二報 Gamma-hydroxybutyric acid (GHB) のヒト下垂体前葉ホルモン分泌に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Gamma-hydroxybutyric acid (GHB), a physiological metabolite of gamma-aminobutyric acid (GABA) was administered to normal persons and patients with hypothalamo-pituitary disorders to examine the effect of GHB on pituitary hormone secretion. GHB stimulated the releases of growth hormone (GH), prolactin (PRL) and cortisol but not luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyrotropin (TSH) in healthy male volunteers. In patients with hypothalamic disorders, plasma GH, PRL and cortisol levels did not increase after GHB administration. In patients with diabetes insipidus, plasma GH responses to GHB were lower than those in normal control, but plasma cortisol and PRL responses were normal. In patients with hypopituitarism, plasma GH and cortisol levels did not elevate after GHB injection, but plasma PRL responses to GHB was normal in three out of patients. In patients with pituitary dwarfism, plasma PRL and cortisol levels increased after GHB injection but plasma GH did not respond to GHB. In patients with acromegaly, plasma GH responses after GHB administration was not consistent. In cases with Cushing's syndrome, plasma cortisol levels increased in patients with adrenocortical hyperplasia but not in those with adrenocortical adenoma. Although plasma GH responses after GHB injection disappeared in patients with Cushing's syndrome, plasma PRL responses was slightly greater than that in normal subjects. In a patient with Addison's disease, plasma ACTH level increased, and plasma PRL and GH responses were normal after GHB injection. To study the relationship between GHB and biogenic amines induced release of human pituitary hormones, the dopaminergic mechanism was blocked by pimozide or stimulated by CB-154 administration, and the serotonergic mechanism was blocked by parachlorophenyl-alanine (PCPA) before GHB administration. GHB induced GH release after treatment with pimozide or PCPA was not significantly different from GH release induced by GHB alone. CB-154 inhibited the increase of plasma GH induced by GHB. Plasma PRL responses to GHB was completely inhibited by CB-154 pretreatment, but not altered by pimozide or PCPA pretreatment. Plasma cortisol responses to GHB was inhibited by pimozide or PCPA pretreatment, but CB-154 had no effect on plasma cortisol responses to GHB injection. GH and cortisol releases induced by GHB was inhibited by continuous TRH infusion. These results suggest that GHB administration is a useful method for examining pituitary hormone secretion. The site of GHB action on pituitary hormone release is probably more central than pituitary in human. Not only inhibition of dopaminergic mechansim but also another mechanism may be involved in GHB induced release of human pituitary hormones.
en-copyright=
kn-copyright=

en-aut-name=YunokiSho
en-aut-sei=Yunoki
en-aut-mei=Sho
kn-aut-name=柚木昌
kn-aut-sei=柚木
kn-aut-mei=昌
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科
en-keyword=GHB
kn-keyword=GHB
en-keyword=ヒト
kn-keyword=ヒト
en-keyword=ＧＨ
kn-keyword=ＧＨ
en-keyword=ＰＲＬ
kn-keyword=ＰＲＬ
en-keyword=Ｃｏｒｔｉｓｏｌ
kn-keyword=Ｃｏｒｔｉｓｏｌ
en-keyword=ＬＨ
kn-keyword=ＬＨ
en-keyword=Pimozide
kn-keyword=Pimozide
en-keyword=PCPA
kn-keyword=PCPA
en-keyword=CB-154
kn-keyword=CB-154
en-keyword=TRH
kn-keyword=TRH
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=891
end-page=897
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies of gamma-aminobutyric acid (GABA) and its metabolite on the control mechanism of secretion of anterior pituitary hormones. Part I: Effects of GABA and gamma-hydroxybutyric acid (GHB) on contents of hypothalamic hormone and secretion of anterior pituitary hormones in rats.
kn-title=Gamma-aminobutyric acid (GABA) 及びその代謝産物の下垂体前葉ホルモン分泌調節における役割に関する研究 第一報 GABA及びGamma-hydroxybutyric acid (GHB) のラット視床下部ホルモン及び下垂体前葉ホルモン分泌に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effects of gamma-aminobutyric acid (GABA), aminooxy acetic acid (AOAA), a GABA transaminase inhibitor, and gamma-hydroxybutyric acid (GHB) on the release of serum growth hormone (GH), prolactin (PRL), luteinizing hormone (LH) and hypothalamic somatotropin release inhibiting hormone (SRIF) content were investigated in urethane or pentobarbital anesthetized Sprague-Dawley male rats. Intraventricular injection of GABA caused a dose-related increase in serum GH and hypothalamic SRIF. In another study, intraperitoneal administration of AOAA produced a significant increase in both serum GH and hypothalamic SRIF. Furthermore, levels of serum GH and hypothalamic SRIF elevated significantly after intraperitoneal GHB administration. Pretreatment with L-DoPA diminished GHB induced serum GH increase and hypothalamic SRIF content. These results showed that GABA and GHB stimulated GH secretion accompanied by increased hypothalamic SRIF. GHB administration caused a significant increase in serum PRL in urethane-anesthetized rats but not in pentobarbital anesthetized rats. Pretreatment with L-DoPA completely inhibited the GHB-induced serum PRL increase in urethane anesthetized rats. Intraperitoneal administration of GHB caused a decrease in serum LH. Preadministration with L-DoPA inhibited GHB induced decrease of serum LH in urethane but not in pentobarbital anesthetized rats. Thus, the GH release induced by GABA or GHB may be partly ascribed to the inhibition of hypothalamic SRIF release. GHB-induced GH release might be mediated by inhibition of dopaminergic mechanism because pretreatment with L-DoPA inhibited GH release after GHB administration. However the mechanism of the GHB-induced serum PRL and LH releases was not clarified in this investigation.
en-copyright=
kn-copyright=

en-aut-name=YunokiSho
en-aut-sei=Yunoki
en-aut-mei=Sho
kn-aut-name=柚木昌
kn-aut-sei=柚木
kn-aut-mei=昌
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科
en-keyword=GABA
kn-keyword=GABA
en-keyword=GHB
kn-keyword=GHB
en-keyword=ラット
kn-keyword=ラット
en-keyword=ソマトスタチン
kn-keyword=ソマトスタチン
en-keyword=ＧＨ
kn-keyword=ＧＨ
en-keyword=ＰＲＬ
kn-keyword=ＰＲＬ
en-keyword=ＬＨ
kn-keyword=ＬＨ
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=881
end-page=889
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Epidemiological and serological studies on influenza Part 1. Plaque cloning from influenza B isolates and cultivation of B type reference strains in the presence of homologous antibody
kn-title=インフルエンザの疫学並びに血清学的研究 ―B型分離株のクローン解析並びにB型代表株の抗血清存在下継代培養実験―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Plaque cloning was conducted with MDCK cells on nine influenza B isolates from 1973 to 1977 in Okayama Prefecture, following hemagglutination inhibition test. Hemagglutinin (HA) antigenicity of clones isolated from two out of four B/Gifu/2/73 like isolates in 1973 varied greatly, including B/Kanagawa/3/76 like viruses, and the clones from B/Kanagawa/3/76 like isolate in 1974 also varied greatly, including B/Kanagawa/3/76 like and its variants. One clone from four B/Kanagawa/3/76 like isolates in 1977 indicated the HA antigenicity of B/Gifu/2/73 variant. After B/Gifu/2/73 and B/Kanagawa/3/76 strains were twice cloned by direct plaque to plaque on MDCK cells, these viruses were cultivated in the presence of homologous antibody. Tenth cultivated viruses and their clones indicated the HA antigenicity of each variant. Hemagglutinin antigenicity of both clones from the isolates and the cultivated viruses in the presence of antibody were stable after fifth cultivation in MDCK cells. From these results, it is suggested that the prevarent virus may be pre-isolated by plaque cloning from and cultivation in the presence of antibody of prevarent isolates.
en-copyright=
kn-copyright=

en-aut-name=HiramatsuMuneshige
en-aut-sei=Hiramatsu
en-aut-mei=Muneshige
kn-aut-name=平松宗成
kn-aut-sei=平松
kn-aut-mei=宗成
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=Influenza virus B type
kn-keyword=Influenza virus B type
en-keyword=Plaque cloning
kn-keyword=Plaque cloning
en-keyword=HA antigenicity
kn-keyword=HA antigenicity
en-keyword=Presence of antibody
kn-keyword=Presence of antibody
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=853
end-page=875
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Total Parenteral Nutrition on Tumor Growth, and Plasma and Tumor Free Amino Acids in Tumor-bearing Rats
kn-title=担癌ラットにおける高カロリー輸液の腫瘍増殖,血漿および腫瘍組織アミノグラムにおよぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study was undertaken to reveal the effects of total parenteral nutrition (TPN) on tumor growth and plasma and tumor free amino acids in male Donryu rats having a transplanted Sato lung cancer. Animals were fed for 7 days and were divided into several groups as follows: 1. regular diet, 2. restricted regular diet, 3. protein free diet, 4.5 % glucose, 5. 21% glucose and 6 to 9. (TPN groups) 21 % glucose and different amino acids. Positive nitrogen balance was gained in TPN groups and body weight gain was observed except in a few cases. There was no stimulation in tumor growth by TPN, which was similar to the regular diet group. Plasma E/N ratio and percentage of blanched chain amino acids were decreased in malnourished rats, but E/N ratio and total amino acid concentration were increased in TPN and the amino acid pattern was similar to the regular diet group. In tumor amino acids analysis, there were no evident deviations of amino acid patterns from plasma amino acids. Constant amino acid trapping was revealed in tumor tissues. Amino acid imbalance therapy was suggested to be effective in the case of deficiencies of Tyr. Glu. and Asp. and high concentration of BCAA.
en-copyright=
kn-copyright=

en-aut-name=UjihiraKatsumi
en-aut-sei=Ujihira
en-aut-mei=Katsumi
kn-aut-name=氏平勝三
kn-aut-sei=氏平
kn-aut-mei=勝三
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第2外科学教室
en-keyword=高カロリー輸液 (Total parenteral nutrition)
kn-keyword=高カロリー輸液 (Total parenteral nutrition)
en-keyword=腫瘍増殖 (Tumor and Nutrition)
kn-keyword=腫瘍増殖 (Tumor and Nutrition)
en-keyword=血漿アミノ酸分析 (Plasma free amino acid)
kn-keyword=血漿アミノ酸分析 (Plasma free amino acid)
en-keyword=腫瘍アミノ酸分析 (Tumor free amino acid)
kn-keyword=腫瘍アミノ酸分析 (Tumor free amino acid)
en-keyword=アミノ酸インパランス (Amino acid imbalance)
kn-keyword=アミノ酸インパランス (Amino acid imbalance)
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=843
end-page=852
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immunological studies on the etiology of gingival hyperplasia induced by chronic administration of Phenytoin Ⅱ. Cell-mediated, delayed hypersensitivity
kn-title=Phenytoinによる歯肉増殖症の発症機序に関する免疫学的検討 第2編 Phenytoinによるアレルギー反応の発現 ―遅延型アレルギーを中心として
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=BSA-Ph is a product of covalent coupling of p-aminophenytoin to bovine serum albumin (BSA). Hartley strain guinea pigs were immunized initially with BSA-Ph in Freund's complete adjuvant and 6 weeks later all animals received a booster injection of the same antigen as an emulsion in Freund's incomplete adjuvant; these injections were administered subcutaneously in the back of the neck and haunches. 4 weeks later, various antigens (BSA-Ph, BSA, EA-Ph and EA) which were dissolved in 0.1 ml of saline were injected in the foot pad of sensitized guinea pigs and the volume of the injected foot was measured at 48 hr after antigen injection. Remarkable swelling of the foot was induced in the animals challenged with BSA-Ph but not with the others. Macrophages obtained from the abdominal cavity of the sensitized animals were injected with various antigens in the skin of normal guinea pigs. At the injection sites, an area of erythema developed and this was followed by the development of indurated nodules over the next 24 to 48 hr. When Ph was given to guinea pigs through the mouth, fairly large amounts of Ph were detected in the gingiva and there was a good correlation between the tissue concentration of Ph and the serum Ph concentration. These findings strongly suggest that delayed-type allergy can be a possible reason for gingival hyperplasia after Ph administration.
en-copyright=
kn-copyright=

en-aut-name=UokawaMasayoshi
en-aut-sei=Uokawa
en-aut-mei=Masayoshi
kn-aut-name=魚川正美
kn-aut-sei=魚川
kn-aut-mei=正美
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学歯学部口腔外科学第1講座
en-keyword=Phenytoin
kn-keyword=Phenytoin
en-keyword=delayed hypersensitivity
kn-keyword=delayed hypersensitivity
en-keyword=gingival hyperplasia
kn-keyword=gingival hyperplasia
en-keyword=MIF
kn-keyword=MIF
en-keyword=antigen-antibody binding
kn-keyword=antigen-antibody binding
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=829
end-page=842
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immunological studies on the etiology of gingival hyperplasia induced by chronic administration of Phenytoin I. Phenytoin radioimmunoassay
kn-title=Phenytoinによる歯肉増殖症の発症機序に関する免疫学的検討 第1編 Phenytoinのradioimmunoassayによる測定法について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The present experiment was initiated in order to study the immunological aspects of the etiology of gingival hyperplasia. To confirm that Phenytoin (Ph) it self can provoke an immune response as partial immunogen in the animal body, the least modified compound of Ph (p-aminophenytoin) was used for bridging with carrier protein (BSA). Rabbits were immunized initially with BSA-Ph in Freund's complete adjuvant and booster injections were carried out with BSA-Ph in Freund's incomplete adjuvant. The radio-immunoassay was carried out by competing unlabelled Ph with a known amount of 3H Ph, and B/T % was measured. The assay can detect as little as 2 μg of Ph. The specificity reaction of antisera with Ph metabolite or the other anticonvulsants revealed that antisera reacted with HPPH(major metabolite of Ph) very slightly but not at all with other anticonvulsants. Parallel analysis performed with HPLC with the same sample revealed excellent correlation between the two methods. The method here described is very simple and provides high accuracy. The success of anti-Ph antisera formation with antigen which provides unmodified Ph as the hapten seems to ensure that BSA-Ph conjugate can be used as a suitable immunogen in provocation of delayed hypersensitivity of Ph.
en-copyright=
kn-copyright=

en-aut-name=UokawaMasayoshi
en-aut-sei=Uokawa
en-aut-mei=Masayoshi
kn-aut-name=魚川正美
kn-aut-sei=魚川
kn-aut-mei=正美
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学歯学部口腔外科学第1講座
en-keyword=Phenytoin
kn-keyword=Phenytoin
en-keyword=Radioimmunoassay
kn-keyword=Radioimmunoassay
en-keyword=HPLC
kn-keyword=HPLC
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=813
end-page=827
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=1982
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies of the Donoggio Reaction and reaction of a small amount of urinary protein Part Ⅵ. Minute amounts of urinary protein in Upper Secontary School Students before and after their physical trainings by improved Iwata's method
kn-title=Donaggio反応及び尿微量蛋白反応に関する研究 第6報 高校生の運動前後の岩田氏改良法による尿微量蛋白測定法とその保存について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to measure the physical load of training of members who belong to the judo and basketball teams, urinary proteins before and after training were measured by the turbidity method (improved iwata's method) of members. Preservation of urine was examined. The following results were obtained: 1) In members of the basketball club, significant increase in actual urinary protein concentration was observed between noon and evening on the first day, between morning and evening on the second day and between morning and noon on the fourth day. Significant increase to protein corrected for urine density of 1.024 was observed between noon and evening on the fourth day. Significant increase in the excretion per minute of urinary protein was observed between noon and evening on the first day, between morning and noon on the fourth day. 2) In members of the judo club, significant increase in actual urinary protein concentration was observed between noon and evening on the first day, between morning and evening on the third day, and between morning and noon on the fourth day. Significant increase in urinary protein concentration corrected to a urine density of 1.024 was observed between noon and evening on the first day, between mouning and evening on the third day, and between morning and noon on the fourth day. Significant increase in the excretion per minute of urinary protein was observed between noon and evening on the first day, and between morning and noon on the fourth day. 3) The main subjective symptoms (from questionaires) which increased with physical loads were "legs getting tired", "the brain feeling hat or muddled", "feeling unsteady in standing" etc in the basketball club, and "legs getting tired", "feeling unsteady in standing", "feeling stiff in the shouldars" etc in the judo club. 4) The preliminary care of fatigued members was for exercise discussed. 5) Iwata's method is a convenient one for determining small amounts of preserved urine after freezing at -20℃ for 30days because the turbiditometric reaction conducted in alkali solution.
en-copyright=
kn-copyright=

en-aut-name=MatsuiYoshinori
en-aut-sei=Matsui
en-aut-mei=Yoshinori
kn-aut-name=松井義典
kn-aut-sei=松井
kn-aut-mei=義典
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=Donaggio反応
kn-keyword=Donaggio反応
en-keyword=岩田氏改良法
kn-keyword=岩田氏改良法
en-keyword=尿微量蛋白
kn-keyword=尿微量蛋白
en-keyword=運動前後尿
kn-keyword=運動前後尿
en-keyword=保存尿
kn-keyword=保存尿
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=805
end-page=812
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=β(2)-Microglobulin in saliva and serum from patients with Sjogren's syndrome
kn-title=シェーグレン病における唾液・血清β(2)ミクログロブリン値
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=β(2)-microglobulin is normally present in low concentrations in serum and other bodily fluids. By use of a radioimmunoassay, we measured salivary and serum β(2)-microglbulin concentrations in 40 patients with Sjogren's syndrome. The β(2)-microglobulin was significantly elevated in the saliva of patients with Sjogren's syndrome (p<0.005), but not in the serum. It was also elevated in the saliva of patients who had severe inflammatory change in the minor salivary gland, abnormal sialographic findings, reduced salivary flow rate and anti SS-A or SS-B antibody in their sera. There was no relation between the salivary , β(2)-microglobulin concentrations and the presence of various autoimmune diseases associated with Sjogren's syndrome. Measurement of salivary β(2)-microglobulin may offer a simple method of quantifying local activity in Sjogren's syndrome.
en-copyright=
kn-copyright=

en-aut-name=NambaYoshio
en-aut-sei=Namba
en-aut-mei=Yoshio
kn-aut-name=難波義夫
kn-aut-sei=難波
kn-aut-mei=義夫
aut-affil-num=1
ORCID=

en-aut-name=KageyamaKeiko
en-aut-sei=Kageyama
en-aut-mei=Keiko
kn-aut-name=景山ケイコ
kn-aut-sei=景山
kn-aut-mei=ケイコ
aut-affil-num=2
ORCID=

en-aut-name=AzukizawaHideo
en-aut-sei=Azukizawa
en-aut-mei=Hideo
kn-aut-name=小豆沢秀夫
kn-aut-sei=小豆沢
kn-aut-mei=秀夫
aut-affil-num=3
ORCID=

en-aut-name=MiyawakiShoji
en-aut-sei=Miyawaki
en-aut-mei=Shoji
kn-aut-name=宮脇昌二
kn-aut-sei=宮脇
kn-aut-mei=昌二
aut-affil-num=4
ORCID=

en-aut-name=OhtaZensuke
en-aut-sei=Ohta
en-aut-mei=Zensuke
kn-aut-name=太田善介
kn-aut-sei=太田
kn-aut-mei=善介
aut-affil-num=5
ORCID=

en-aut-name=OfujiTadashi
en-aut-sei=Ofuji
en-aut-mei=Tadashi
kn-aut-name=大藤眞
kn-aut-sei=大藤
kn-aut-mei=眞
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科

affil-num=2
en-affil=
kn-affil=岡山大学医学部第三内科

affil-num=3
en-affil=
kn-affil=岡山大学医学部第三内科

affil-num=4
en-affil=
kn-affil=岡山大学医学部第三内科

affil-num=5
en-affil=
kn-affil=岡山大学医学部第三内科

affil-num=6
en-affil=
kn-affil=岡山大学学長
en-keyword=Sjögren's syndrome
kn-keyword=Sjögren's syndrome
en-keyword=β(2)-microglobulin
kn-keyword=β(2)-microglobulin
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=791
end-page=804
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of total parental nutrition on body weight, tumor growth, plasma aminogram, tumor aminogram, nitrogen balance and histological finding in tumor bearing rats
kn-title=高カロリー輸液の癌治療への応用に関する実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study was undertaken to examine the effect of parenteral nutrition upon body weight, tumor growth, plasma aminogram, tumor aminogram, nitrogen balance and histological change in rats with a transplanted tumor. Twenty-five male Donryu rats, having a subcutaneously transplanted Sato Lung Cancer were studied. They were divided into 5 groups. A silastic catheter was inserted into the superior vena cava via the left common jugular vein of rats of the infused group. Infusion continued for 7 days. Tumor size was three-dimensionally measured. All rats were sacrificed on the 8th day. The body weght increase in group I., Ⅱ., Ⅲ., Ⅳ and Ⅴ was 35.0g, 35.4g, -69.0g, 2.8g and -19.8g. The tumor growth rate in group I. Ⅱ. Ⅲ. Ⅳ and Ⅴ was 10.06, 4.15, 10.07, 8.01 and 4.75 respectively. Plasma aminogram showed markedly elevated levels of Thr in group Ⅳ, Ⅴ and Ser, Gly, Lvs, in group Ⅲ. On the contrary, lower levels of Val, Leu, Ile were found in group Ⅲ. ACNU had no effect on plasma aminogram. There was no difference between TPN and oral feeding as far as tumor aminogram was concerned. The nitrogen balance study in group Ⅲ revealed massive necrosis and small sized tumor cells. Tumor cells of group Ⅳ showed large multinclear cells and distinct chromatin. There was no significant difference between group Ⅱ and Ⅴ as far as anti-neoplastic effect of ACNU. Provision of adequate nutrition intravenously to tumor-bearing animals restores body weght and positive nitrogen balance. TPN has significant effect on intensive neoplastic therapy.
en-copyright=
kn-copyright=

en-aut-name=OhnishiMasataka
en-aut-sei=Ohnishi
en-aut-mei=Masataka
kn-aut-name=大西正高
kn-aut-sei=大西
kn-aut-mei=正高
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第2外科学教室
en-keyword=完全静脈栄養
kn-keyword=完全静脈栄養
en-keyword=担癌ラット
kn-keyword=担癌ラット
en-keyword=ＡＣＮＵ
kn-keyword=ＡＣＮＵ
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=783
end-page=790
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immunochemotherapy with tumor antibody-Neocarzinostatin (NCS) conjugates. Part Ⅱ. Biological activities of antibody-NCS conjugates (NCS-immune IgG)
kn-title=抗腫瘍抗体結合Neocarzinostatin(NCS)による免疫化学療法の研究 第2編 抗体結合Neocarzinostatin(NCS-immune IgG)の生物学的活性の検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Anti-NALL-1 rabbit IgG was incubated with NCS, an unique membrane-reactive anticancer antibiotic, in the presence of water-soluble carbodiimide. The resulting mixture contained NCS-immune IgG. The conjugates inhibited the growth and (3)H-TdR incorporation of human leukemia cell line (NALL-1) cells. A membrane immunofluorescent test with FITC-labeled rabbit anti-NCS and goat anti-rabbit IgG antibodies showed specific localization of NCS-immune IgG on NALL-1 cell surfaces. These results indicate that NCS-immune IgG retains both NCS and antibody activities, and NCS-immune IgG should be useful for cancer therapy.
en-copyright=
kn-copyright=

en-aut-name=KobayashiTakashi
en-aut-sei=Kobayashi
en-aut-mei=Takashi
kn-aut-name=古林太加志
kn-aut-sei=古林
kn-aut-mei=太加志
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第2内科
en-keyword=免疫化学療法
kn-keyword=免疫化学療法
en-keyword=抗腫瘍抗体結合抗癌剤
kn-keyword=抗腫瘍抗体結合抗癌剤
en-keyword=Neocarzinostatin
kn-keyword=Neocarzinostatin
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=9-10
article-no=
start-page=775
end-page=782
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19821030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A intestinal anisakiasis with a ileus.
kn-title=腸アニサキス症について ―その症例を中心として―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A case of ileus caused by intestinal anisakiasis is described in this paper. The intestine manifested a typical allergic inflamation. Pathological changes such as extensive edema 2nd fibrosis were found in the intestine. Eosinophiles, neutrophiles and monocytes also infiltrated near the site of the penetrating worm. The worm from the intestine of the patient was identified with a SEM. On the basis of the available evidence, it can be safely said that this worm is Anisakis type I larva, although the mucro on the tip of larval tail was not clear. The patient may have been infected with Anisakis larva by eating "SASHIMI" of a small yellowtail (HAMACHI) from the Seto Inland Sea, because these small yellowtail were fed fish caught near Hokkaido, Tohoku and San-in.
en-copyright=
kn-copyright=

en-aut-name=AjiToshiki
en-aut-sei=Aji
en-aut-mei=Toshiki
kn-aut-name=安治敏樹
kn-aut-sei=安治
kn-aut-mei=敏樹
aut-affil-num=1
ORCID=

en-aut-name=FukudaTomio
en-aut-sei=Fukuda
en-aut-mei=Tomio
kn-aut-name=福田富男
kn-aut-sei=福田
kn-aut-mei=富男
aut-affil-num=2
ORCID=

en-aut-name=HoLi Shin
en-aut-sei=Ho
en-aut-mei=Li Shin
kn-aut-name=何黎星
kn-aut-sei=何
kn-aut-mei=黎星
aut-affil-num=3
ORCID=

en-aut-name=TonguYasumasa
en-aut-sei=Tongu
en-aut-mei=Yasumasa
kn-aut-name=頓宮廉正
kn-aut-sei=頓宮
kn-aut-mei=廉正
aut-affil-num=4
ORCID=

en-aut-name=InatomiSeiiti
en-aut-sei=Inatomi
en-aut-mei=Seiiti
kn-aut-name=稲臣成一
kn-aut-sei=稲臣
kn-aut-mei=成一
aut-affil-num=5
ORCID=

en-aut-name=DoiKenji
en-aut-sei=Doi
en-aut-mei=Kenji
kn-aut-name=土井謙司
kn-aut-sei=土井
kn-aut-mei=謙司
aut-affil-num=6
ORCID=

en-aut-name=MotoiMakoto
en-aut-sei=Motoi
en-aut-mei=Makoto
kn-aut-name=元井信
kn-aut-sei=元井
kn-aut-mei=信
aut-affil-num=7
ORCID=

en-aut-name=KoshimuneIichiro
en-aut-sei=Koshimune
en-aut-mei=Iichiro
kn-aut-name=越宗猪一郎
kn-aut-sei=越宗
kn-aut-mei=猪一郎
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部寄生虫学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部寄生虫学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部寄生虫学教室

affil-num=4
en-affil=
kn-affil=岡山大学医学部寄生虫学教室

affil-num=5
en-affil=
kn-affil=岡山大学医学部寄生虫学教室

affil-num=6
en-affil=
kn-affil=岡山大学医学部第二病理学教室

affil-num=7
en-affil=
kn-affil=岡山大学医学部第二病理学教室

affil-num=8
en-affil=
kn-affil=越宗医院
en-keyword=腸アニサキス症
kn-keyword=腸アニサキス症
en-keyword=腸閉塞
kn-keyword=腸閉塞
en-keyword=アレルギー性炎症
kn-keyword=アレルギー性炎症
en-keyword=アニサキスⅠ型幼虫
kn-keyword=アニサキスⅠ型幼虫
END