start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=525 end-page=531 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of pulmonary bronchogenic cyst complicated by pulmonary tuberculosis kn-title=診断困難であった肺結核と気管支性肺嚢胞の合併症例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 53 year old male was admitted to the Okayama University Medical School Hospital in June 1975 with a history of chronic cough and sputum. The patient had previously undergone treatment for pulmonary tuberculosis for 18 months at another hospital. Roentgenologic examination showed a lobulated mass and fibrotic density in the left upper lung field. Left upper lobectomy was performed. The histopathologic diagnosis was pulmonary bronchogenic cyst with pulmonary tuberculosis in the same segment. In this report, the roentgenological findings and differential diagnosis of pulmonary bronchogenic cyst are described. en-copyright= kn-copyright= en-aut-name=MorinoYasuo en-aut-sei=Morino en-aut-mei=Yasuo kn-aut-name=森野靖雄 kn-aut-sei=森野 kn-aut-mei=靖雄 aut-affil-num=1 ORCID= en-aut-name=KimotoShin en-aut-sei=Kimoto en-aut-mei=Shin kn-aut-name=木本真 kn-aut-sei=木本 kn-aut-mei=真 aut-affil-num=2 ORCID= en-aut-name=SatoKatashi en-aut-sei=Sato en-aut-mei=Katashi kn-aut-name=佐藤功 kn-aut-sei=佐藤 kn-aut-mei=功 aut-affil-num=3 ORCID= en-aut-name=HashimotoKeiji en-aut-sei=Hashimoto en-aut-mei=Keiji kn-aut-name=橋本啓二 kn-aut-sei=橋本 kn-aut-mei=啓二 aut-affil-num=4 ORCID= en-aut-name=MizukawaKiichiro en-aut-sei=Mizukawa en-aut-mei=Kiichiro kn-aut-name=水川帰一郎 kn-aut-sei=水川 kn-aut-mei=帰一郎 aut-affil-num=5 ORCID= en-aut-name=MorimotoSetsuo en-aut-sei=Morimoto en-aut-mei=Setsuo kn-aut-name=森本節夫 kn-aut-sei=森本 kn-aut-mei=節夫 aut-affil-num=6 ORCID= en-aut-name=TamaiToyosato en-aut-sei=Tamai en-aut-mei=Toyosato kn-aut-name=玉井豊理 kn-aut-sei=玉井 kn-aut-mei=豊理 aut-affil-num=7 ORCID= en-aut-name=AonoKaname en-aut-sei=Aono en-aut-mei=Kaname kn-aut-name=青野要 kn-aut-sei=青野 kn-aut-mei=要 aut-affil-num=8 ORCID= en-aut-name=TanabeMasatada en-aut-sei=Tanabe en-aut-mei=Masatada kn-aut-name=田辺正忠 kn-aut-sei=田辺 kn-aut-mei=正忠 aut-affil-num=9 ORCID= en-aut-name=ItagakiFumio en-aut-sei=Itagaki en-aut-mei=Fumio kn-aut-name=板垣文夫 kn-aut-sei=板垣 kn-aut-mei=文夫 aut-affil-num=10 ORCID= en-aut-name=SimizuNobuyoshi en-aut-sei=Simizu en-aut-mei=Nobuyoshi kn-aut-name=清水信義 kn-aut-sei=清水 kn-aut-mei=信義 aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=2 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=3 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=4 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=5 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=6 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=7 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=8 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=9 en-affil= kn-affil=岡山大学医学部放射線科 affil-num=10 en-affil= kn-affil=岡山大学医学部第II外科 affil-num=11 en-affil= kn-affil=岡山大学医学部第II外科 en-keyword=Bronchogenic cyst kn-keyword=Bronchogenic cyst en-keyword=Pulmonary tuberculosis kn-keyword=Pulmonary tuberculosis END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=519 end-page=524 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The relationship between incurable gastric ulcer and Candida albicans at the ulcer base II. Immuno-fluorescent study of experimentally-induced gastric ulcer in rats using anti-Candida albicans antisera kn-title=難治性胃潰瘍とCandida albicansとの関り合いに関する研究 第II編 Rat実験胃瘍底部真菌の免疫組織学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Experimental gastric ulcer was induced in Wistar rats by a Clamping-Cortisone method. Candida albicans infection was achieved by oral administration. Animals were sacrificed after the 4th and 13th week. Gastric ulcers were investigated by immuno-fluorescence using anti-Candida albicans antisera. In rats sacrificed after the 4th week, Candida albicans was fluorescent positive at the ulcer base. As in man, many fluorescent histiocytes were seen not only around, but far from the ulcerated lesions. Inflammatory cells were also numerous at the ulcer base. These findings support the hypothesis that, in man Candida albicans infection at the ulcer base is not merely a simple growth, but that it is also involved in the poor curability of gastric ulcer. In animals sacrificed at the 13th week, fibrous change with collagen formation at the ulcer base was less prominent than in those sacrificed at the same period but not infected by Candida albicans. Possibly, this is due to the fact that Candida albicans inhibits fibrous change and collagen formation (mechanisms of healing) in the gastric ulcer. en-copyright= kn-copyright= en-aut-name=HitomiFumio en-aut-sei=Hitomi en-aut-mei=Fumio kn-aut-name=人見文雄 kn-aut-sei=人見 kn-aut-mei=文雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科学教室 en-keyword=Clamping-Cortisone法 kn-keyword=Clamping-Cortisone法 en-keyword=Candida allicans kn-keyword=Candida allicans en-keyword=螢光抗体法 kn-keyword=螢光抗体法 en-keyword=組織球 kn-keyword=組織球 en-keyword=膠原線維発育度 kn-keyword=膠原線維発育度 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=511 end-page=518 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The relationship between incurable gastric ulcer and Candida albicans at the ulcer base I. Immuno-fluorescent study of human gastric ulcer using anti-Candida albicans antisera kn-title=難治性胃潰瘍とCandida albicansとの関り合いに関する研究 第I編 螢光抗体法によるヒト胃潰瘍組織部Candida albicansの免疫組織学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Forty-seven cases of gastric ulcer including some with ulcerated carcinoma were investigated by an immuno-fluorescent technique using anti-Candida albicans antisera. Fresh tissue containing ulcer base was also stained with PAS reagent. Candida albicans at the ulcer base was positive for fluorescence. The cytoplasm of many histiocytes was also fluorescent. Histiocytes were predominant at the ulcer base and were present even in the muscular and subserosal layers. The cytoplasm of these cells was PAS-positive. These data suggest that Candida albicans at the necrotic ulcer base is not a simple opportunistic growth, but that some of its watersoluble factor may be involved in the poor curability of gastric ulcer. en-copyright= kn-copyright= en-aut-name=HitomiFumio en-aut-sei=Hitomi en-aut-mei=Fumio kn-aut-name=人見文雄 kn-aut-sei=人見 kn-aut-mei=文雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科学教室 en-keyword=難治性潰瘍 kn-keyword=難治性潰瘍 en-keyword=Candiba albicans kn-keyword=Candiba albicans en-keyword=螢光抗体法 kn-keyword=螢光抗体法 en-keyword=可溶性抗原 kn-keyword=可溶性抗原 en-keyword=組織球 kn-keyword=組織球 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=503 end-page=509 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Treatment of head and neck malignant lymphoma -Results with B-COP therapy in combination with radiotherapy- kn-title=頭頸部悪性リンパ腫の治療 ―放射線療法と多剤併用B-COP療法の成績― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Fifteen patients with head and neck malignant lymphomas have been treated with a combination of bleomycin, cyclophosphamide, oncovin (vincristine) and prednisolone (B-COP) in combination with radiotherapy since 1975. The results are discussed and compared with those of eleven patients treated with radiotherapy alone. Complete remission and its duration in stage II E were significantly superior to those treated with radiation alone. Toxicity, chiefly leukopenia, was generally tolerable and reversible. Although the number of cases and the follow-up period are inadequate as yet, this therapeutic regimen is a promising means of increasing the duration of remission and the survival of patients with head and neck malignant lymphomas. en-copyright= kn-copyright= en-aut-name=SaitoRyusuke en-aut-sei=Saito en-aut-mei=Ryusuke kn-aut-name=斉藤龍介 kn-aut-sei=斉藤 kn-aut-mei=龍介 aut-affil-num=1 ORCID= en-aut-name=EndoYoichi en-aut-sei=Endo en-aut-mei=Yoichi kn-aut-name=遠藤洋一 kn-aut-sei=遠藤 kn-aut-mei=洋一 aut-affil-num=2 ORCID= en-aut-name=AokiTeruyuki en-aut-sei=Aoki en-aut-mei=Teruyuki kn-aut-name=青木輝行 kn-aut-sei=青木 kn-aut-mei=輝行 aut-affil-num=3 ORCID= en-aut-name=FujimotoAkiko en-aut-sei=Fujimoto en-aut-mei=Akiko kn-aut-name=藤本明子 kn-aut-sei=藤本 kn-aut-mei=明子 aut-affil-num=4 ORCID= en-aut-name=AkagiSeiko en-aut-sei=Akagi en-aut-mei=Seiko kn-aut-name=赤木成子 kn-aut-sei=赤木 kn-aut-mei=成子 aut-affil-num=5 ORCID= en-aut-name=MasudaYu en-aut-sei=Masuda en-aut-mei=Yu kn-aut-name=増田游 kn-aut-sei=増田 kn-aut-mei=游 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部耳鼻咽喉科学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部耳鼻咽喉科学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部耳鼻咽喉科学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部耳鼻咽喉科学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部耳鼻咽喉科学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部耳鼻咽喉科学教室 en-keyword=悪性リンパ腫 kn-keyword=悪性リンパ腫 en-keyword=頭頸部 kn-keyword=頭頸部 en-keyword=併用療法 kn-keyword=併用療法 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=499 end-page=502 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Fluorometric analysis of galactose in whole blood spotted on paper kn-title=血液濾紙のガラクトースの定量法 en-subtitle= kn-subtitle= en-abstract=Galactose was measured in whole blood spotted on paper as a screening test for congenital metabolic disorders in the newborn. Two dried blood spots of 3 mm diameter were extracted with 2% perchloric acid and diluted with a buffer. Galactose was determined by galactose dehydrogenase. The normal range for galactose in the newborn by this method was 0 to 0.15 mg/dl. kn-abstract=新生児の血液を滲み込ませた濾紙中のガラクトースを2%過塩素酸で抽出してga1actoseを測定する方法について検討した.この方法は除蛋白操作が容易で,安定した結果が得られるので血中ガラクトースの定量方法として有用である.本法による新生児血中ガラクトースの平均値は0.14mg/dlであり,生後の日齢による変動は見られなかった. en-copyright= kn-copyright= en-aut-name=MisumiHiromasa en-aut-sei=Misumi en-aut-mei=Hiromasa kn-aut-name=美澄博雅 kn-aut-sei=美澄 kn-aut-mei=博雅 aut-affil-num=1 ORCID= en-aut-name=SuemasaKeiko en-aut-sei=Suemasa en-aut-mei=Keiko kn-aut-name=末政恵子 kn-aut-sei=末政 kn-aut-mei=恵子 aut-affil-num=2 ORCID= en-aut-name=DoiMasato en-aut-sei=Doi en-aut-mei=Masato kn-aut-name=土居真人 kn-aut-sei=土居 kn-aut-mei=真人 aut-affil-num=3 ORCID= en-aut-name=InoueYuri en-aut-sei=Inoue en-aut-mei=Yuri kn-aut-name=井上百合 kn-aut-sei=井上 kn-aut-mei=百合 aut-affil-num=4 ORCID= en-aut-name=IshidaTatsuo en-aut-sei=Ishida en-aut-mei=Tatsuo kn-aut-name=石田立夫 kn-aut-sei=石田 kn-aut-mei=立夫 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山県環境保健センター affil-num=2 en-affil= kn-affil=岡山県環境保健センター affil-num=3 en-affil= kn-affil=岡山県環境保健センター affil-num=4 en-affil= kn-affil=岡山県環境保健センター affil-num=5 en-affil= kn-affil=岡山県環境保健センター en-keyword=全血中のガラクトース kn-keyword=全血中のガラクトース en-keyword=血液濾紙 kn-keyword=血液濾紙 en-keyword=ガラクトースデビドロゲナーゼ kn-keyword=ガラクトースデビドロゲナーゼ END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=491 end-page=497 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of 1, 10-phenanthroline on RNA polymerase from mouse ascites sarcoma cells kn-title=RNAポリメラーゼに対するZnキレート剤: 1, 10-phenanthrolineの作用 en-subtitle= kn-subtitle= en-abstract=The effects of a zinc chelator, 1, 10-phenanthroline on RNA polymerase from RSV-induced mouse ascites sarcoma cells were studied. RNA polymerase I was inhibited at the initiation step by 2 mM 1, 10-phenanthroline. RNA polymerase II was not inhibited. The enzymes were not inhibited by a related compound, 4, 7-dimethyl-1, 10-phenanthroline, which is not a zinc chelating agent. The inhibition of RNA polymerase I was reversible. The chelating agent reduced the Km value for nucleoside triphosphates and inhibited the RNA polymerase I at the first phosphodiester bond formation step. Endogenous RNA polymerase I activity in nucleotide permeable cells (prepared by treatment of SR-C3H/He cells in hypotonic buffer) was inhibited by 1, 10-phenanthroline. The results suggest that RNA chain initiation at the first phosphodiester formation with RNA polymerase I occur in permeable SR-C3H/He cells. kn-abstract=マウス腹水肉腫細胞より分離したRNAポリメラーゼI, IIに対する亜鉛のキレート剤, 1, 10-phe-nanthroline (OP) の作用を検討した.RNAポリメラーゼI活性を50%阻害するOP濃度は2mMであった.RNAポリメラ-ゼII活性はOPで阻害されなかった.RNAポリメラーゼとOPとを予めインキュベートしても,阻害の程度は変化せず, OPを除去すると阻害は回復することから, OPはポリメラーゼ分子内に強く結合している亜鉛に可逆的に結合することが示唆された.OPはRNAポリメラーゼIの転写開始反応を選択的に阻害することが示唆された.核内でクロマチンに結合したRNAポリメラ-ゼIの活性もOPによって分離酵素と同程度に阻害され,OPの結合部位が,分離ポリメラーゼIと同様にOPと結合できる状態にあることが示唆された. en-copyright= kn-copyright= en-aut-name=MisumiHiromasa en-aut-sei=Misumi en-aut-mei=Hiromasa kn-aut-name=美澄博雅 kn-aut-sei=美澄 kn-aut-mei=博雅 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌研生化学 en-keyword=RNAポリメラーゼ kn-keyword=RNAポリメラーゼ en-keyword=Zn結合酵素 kn-keyword=Zn結合酵素 en-keyword=Znキレーター kn-keyword=Znキレーター en-keyword=1, 10-フェナンスロリン kn-keyword=1, 10-フェナンスロリン END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=469 end-page=490 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The fine structure of medulloblastoma -With special reference to the histological pattern- kn-title=髄芽腫の超微形態 ―特に組織像との関連について― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ten cases of human cerebellar medulloblastomas were examined by light and electron microscopy. Ultrastructural differentiation of tumor cells was discussed in relation to the histological patterns. In areas which appeared densely cellular with light microscopy, the tumor was composed of a series of undifferentiated cells having a variety of junctions between tumor cells. In areas showing rosette formation with light microscopy, many elongated cytoplasmic processes and numerous mutually entangled intracytoplasmic microtubules were seen in the center of Homer-Wright pseudorosettes. In the area seen as a whorled pattern by light microscopy, elongated fine cytoplasmic processes showing a lamellar array were prominent. In the spongioblastic parts with PTAH-positive fibrils, intracytoplasmic glial filaments were not observed by electron microscopy even though a few fine fibrillary structures were present. The formation of solitary cilia was observed only in the desmoplastic area by light microscopy. Therefore, the medulloblastoma was composed of extremely undifferentiated cells which only a little neuroblastic differentiation. This was limited to the rosette forming areas. en-copyright= kn-copyright= en-aut-name=YasudaHideki en-aut-sei=Yasuda en-aut-mei=Hideki kn-aut-name=安田英己 kn-aut-sei=安田 kn-aut-mei=英己 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二病理学教室 en-keyword=髄芽腫 kn-keyword=髄芽腫 en-keyword=超微形態 kn-keyword=超微形態 en-keyword=組織像との関連 kn-keyword=組織像との関連 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=461 end-page=467 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Analysis of the action of Illudin S: Effects of Illudin S on surface structures of L(929) fibroblasts kn-title=Illudin Sの作用機作の解折:Illudin SのL(929)線維芽細胞の表面形態に及ぼす影響について en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of Illudin S (a toadstool poison) on surface structures of L(929) mouse fibroblasts were studied by scanning electron microscopy. Illudin S in a concentration of 1 ng/ml strongly inhibited cell proliferation, but had no effect on surface structures of L cells through all the cell phases. These results indicate that the biological effects of Illudin S were not manifested by changes in the cytoskeletal system, the cell ability to attach to the substrate, of the surface structures of the cell. It is suggested that the cellular sensitive sites to Illudin S are inside the cell. en-copyright= kn-copyright= en-aut-name=KuramitsuMakoto en-aut-sei=Kuramitsu en-aut-mei=Makoto kn-aut-name=倉光誠 kn-aut-sei=倉光 kn-aut-mei=誠 aut-affil-num=1 ORCID= en-aut-name=ItanoToshifumi en-aut-sei=Itano en-aut-mei=Toshifumi kn-aut-name=板野俊文 kn-aut-sei=板野 kn-aut-mei=俊文 aut-affil-num=2 ORCID= en-aut-name=MatsuiHideki en-aut-sei=Matsui en-aut-mei=Hideki kn-aut-name=松井秀樹 kn-aut-sei=松井 kn-aut-mei=秀樹 aut-affil-num=3 ORCID= en-aut-name=TokudaMasaaki en-aut-sei=Tokuda en-aut-mei=Masaaki kn-aut-name=徳田雅明 kn-aut-sei=徳田 kn-aut-mei=雅明 aut-affil-num=4 ORCID= en-aut-name=HataseOsamu en-aut-sei=Hatase en-aut-mei=Osamu kn-aut-name=畠瀬修 kn-aut-sei=畠瀬 kn-aut-mei=修 aut-affil-num=5 ORCID= en-aut-name=MurakamiTetsuhide en-aut-sei=Murakami en-aut-mei=Tetsuhide kn-aut-name=村上哲英 kn-aut-sei=村上 kn-aut-mei=哲英 aut-affil-num=6 ORCID= en-aut-name=NisidaIsamu en-aut-sei=Nisida en-aut-mei=Isamu kn-aut-name=西田勇 kn-aut-sei=西田 kn-aut-mei=勇 aut-affil-num=7 ORCID= en-aut-name=HayashiHideo en-aut-sei=Hayashi en-aut-mei=Hideo kn-aut-name=林英生 kn-aut-sei=林 kn-aut-mei=英生 aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=8 en-affil= kn-affil=岡山大学医学部細菌学教室 en-keyword=Illudin S kn-keyword=Illudin S en-keyword=細胞表面形態 kn-keyword=細胞表面形態 en-keyword=L細胞 kn-keyword=L細胞 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=453 end-page=460 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Illudin S; a possible new type inhibitor of RNA synthesis, and analysis of its action mechanism kn-title=新しいRNA合成阻害剤Illudin Sの作用機作の解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Illudin S primarily and specificially inhibited RNA synthesis at a concentration of 1-10ng/ml of culture medium in the early period of G(1)-S phase transition as well as in S phase in a growing medium. DNA synthesis in S phase and cell proliferation were inhibited as secondary events. The inhibitory effects on RNA synthesis were only manifested in intact cell systems, not in cell-free ones. DNA replication in a nucleotide-permeable cell system was completely insensitive to Illudin S. Protein synthesis in intact cell systems was not inhibited by Illudin S. Illudin S should prove useful in the analysis of the mechanism of G(l)-S phase transition in the cell cycle; especially in the elucidation of specific kinds of RNA required for initiation of DNA replication. en-copyright= kn-copyright= en-aut-name=ItanoToshifumi en-aut-sei=Itano en-aut-mei=Toshifumi kn-aut-name=板野俊文 kn-aut-sei=板野 kn-aut-mei=俊文 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 en-keyword=Illudin S kn-keyword=Illudin S en-keyword=RNA合成 kn-keyword=RNA合成 en-keyword=DNA合成 kn-keyword=DNA合成 en-keyword=細胞増殖 kn-keyword=細胞増殖 en-keyword=線維芽細胞 kn-keyword=線維芽細胞 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=437 end-page=452 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Intracranial pressure differences associated with cold-induced cerebral edema kn-title=脳浮腫における局所頭蓋内圧差発生に関する実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The present studies were designed to determine whether significant pressure differences between hemispheres occur in response to cold-induced cerebral edema and if so, their response to induced high intracranial pressure. The experiments were carried out on 35 adult mongrel dogs weighing between 8 to 12kg under intraperitoneal pentobarbital anesthesia. A cold-induced edema was produced at the left frontal lobe by placing a metallic cylinder filled with liquid nitrogen on the exposed dura mater for 5 minutes. Subdural pressures were recorded continuously for 12 hours from the subdural spaces over the left parietal lobe adjacent to the cold injury and the right parietal lobe remote to the injury, using microballoons or Fiber-optic pressure switch sensors through burr holes. Cisterns magna pressure was also continuously measured with a needle inserted into the cisterna magna by percutaneous puncture. In addition, during progressing cerebral edema, intracranial pressure was investigated after the injection of saline solution into the cisterna magna at 1000 mm hydrostatic pressure at 3, 6 and 12 hours after cold injury. Three hours after cold injury, the subdural pressure of the left injured hemisphere was higher than that of the right non-injured one. About 6-9 hours following injury, pressure differences between injured and non-injured hemispheres showed maximal values ranging from 40 to 160 mm H(2)O (3-11.8 mmHg). Intracranial pressure had increased markedly. by 9 hours after cold injury, at which time such pressure differences decreased gradually. In the late stage of edema, however, intracranial pressure was distributed equally throughout the entire brain, so that pressure differences had disappeared. The changes in intracranial pressure after injection of saline revealed a biphasic pattern about 3 hours after injury as compared with the normal. At 12 hours, elevated intracranial pressure after injection did not return to the previous resting level. These phenomina suggest that the initial steep drop represented intracranial volumetric compensation by the intracranial and spinal coverings and venous system in the central nervous system, and that the late gentle slope represented impairment of cerebrospinal fluid absorption. These results indicated at least two possible explanations for the origin of pressure differences : 1) reduction of the pressure compensating capacity of the injured swollen hemisphere blocking the free communication of cerebrospinal fluid and collapsed venous system, 2) delayed transmission of the elevated pressure from the lesion to the noninjured hemisphere because of local change in viscoelasticity. Accordingly, changes in pressure differences as well as the height of intracranial pressure would play a major role in the pathophysiology of an expanding lesion. en-copyright= kn-copyright= en-aut-name=NinomiyaKazuhiko en-aut-sei=Ninomiya en-aut-mei=Kazuhiko kn-aut-name=二宮一彦 kn-aut-sei=二宮 kn-aut-mei=一彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳神経外科 en-keyword=cold-induced edema kn-keyword=cold-induced edema en-keyword=subdural pressure kn-keyword=subdural pressure en-keyword=intracranial pressure difference kn-keyword=intracranial pressure difference en-keyword=cisterna magna pressure kn-keyword=cisterna magna pressure END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=425 end-page=436 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of fulminant hepatitis with electrocardiographic changes simulating acute myocardial infarction kn-title=経過中に心筋硬塞様心電図(Q波, ST上昇)を示した劇症肝炎の1例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Serial elctrocardiograms of a 55 year old female showed a trasiently elevated ST-T wave and decreased R waves in right precordial leads suggesting anterior wall infraction during the course of fulminant hepatitis. Autopsy findings revealed marked cerebral edema, massive necrosis of the liver with lymphocytic infiltration, partial myocardial degeneration and necrosis with infiltration by lymphocytes and plasma cells. However, no significant lesions were found in the coronary arterial trees. These findings suggest that the electrocardiographic changes were due to catecholamine cardiomyopathy or viral myocarditis. en-copyright= kn-copyright= en-aut-name=YoshidaHidenori en-aut-sei=Yoshida en-aut-mei=Hidenori kn-aut-name=吉田英紀 kn-aut-sei=吉田 kn-aut-mei=英紀 aut-affil-num=1 ORCID= en-aut-name=KusuharaShunichi en-aut-sei=Kusuhara en-aut-mei=Shunichi kn-aut-name=楠原俊一 kn-aut-sei=楠原 kn-aut-mei=俊一 aut-affil-num=2 ORCID= en-aut-name=YoshiokaNobuhiko en-aut-sei=Yoshioka en-aut-mei=Nobuhiko kn-aut-name=吉岡信彦 kn-aut-sei=吉岡 kn-aut-mei=信彦 aut-affil-num=3 ORCID= en-aut-name=KusachiShowzo en-aut-sei=Kusachi en-aut-mei=Showzo kn-aut-name=草地省藏 kn-aut-sei=草地 kn-aut-mei=省藏 aut-affil-num=4 ORCID= en-aut-name=SogoTaiji en-aut-sei=Sogo en-aut-mei=Taiji kn-aut-name=十河泰司 kn-aut-sei=十河 kn-aut-mei=泰司 aut-affil-num=5 ORCID= en-aut-name=YamadaNobuyuki en-aut-sei=Yamada en-aut-mei=Nobuyuki kn-aut-name=山田信行 kn-aut-sei=山田 kn-aut-mei=信行 aut-affil-num=6 ORCID= en-aut-name=NishiyamaOsamu en-aut-sei=Nishiyama en-aut-mei=Osamu kn-aut-name=西山修 kn-aut-sei=西山 kn-aut-mei=修 aut-affil-num=7 ORCID= en-aut-name=YasuharaKoichiro en-aut-sei=Yasuhara en-aut-mei=Koichiro kn-aut-name=安原耕一郎 kn-aut-sei=安原 kn-aut-mei=耕一郎 aut-affil-num=8 ORCID= en-aut-name=UedaMinoru en-aut-sei=Ueda en-aut-mei=Minoru kn-aut-name=上田稔 kn-aut-sei=上田 kn-aut-mei=稔 aut-affil-num=9 ORCID= en-aut-name=SaitoDaiji en-aut-sei=Saito en-aut-mei=Daiji kn-aut-name=斉藤大治 kn-aut-sei=斉藤 kn-aut-mei=大治 aut-affil-num=10 ORCID= en-aut-name=MaeRitsuo en-aut-sei=Mae en-aut-mei=Ritsuo kn-aut-name=前律夫 kn-aut-sei=前 kn-aut-mei=律夫 aut-affil-num=11 ORCID= en-aut-name=ShimadaYoshihiro en-aut-sei=Shimada en-aut-mei=Yoshihiro kn-aut-name=島田宜浩 kn-aut-sei=島田 kn-aut-mei=宜浩 aut-affil-num=12 ORCID= en-aut-name=MatsuuraHidemitsu en-aut-sei=Matsuura en-aut-mei=Hidemitsu kn-aut-name=松浦秀光 kn-aut-sei=松浦 kn-aut-mei=秀光 aut-affil-num=13 ORCID= en-aut-name=OmoriHiroyuki en-aut-sei=Omori en-aut-mei=Hiroyuki kn-aut-name=大森浩之 kn-aut-sei=大森 kn-aut-mei=浩之 aut-affil-num=14 ORCID= en-aut-name=KimotoKatsuhiko en-aut-sei=Kimoto en-aut-mei=Katsuhiko kn-aut-name=木本克彦 kn-aut-sei=木本 kn-aut-mei=克彦 aut-affil-num=15 ORCID= en-aut-name=ShigeiHiroshi en-aut-sei=Shigei en-aut-mei=Hiroshi kn-aut-name=重井博 kn-aut-sei=重井 kn-aut-mei=博 aut-affil-num=16 ORCID= en-aut-name=HaraokaShoichi en-aut-sei=Haraoka en-aut-mei=Shoichi kn-aut-name=原岡昭一 kn-aut-sei=原岡 kn-aut-mei=昭一 aut-affil-num=17 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=2 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=3 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=4 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=5 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=6 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=7 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=8 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=9 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=10 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=11 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=12 en-affil= kn-affil=岡山大学医学部第一内科 affil-num=13 en-affil= kn-affil=重井病院内科 affil-num=14 en-affil= kn-affil=重井病院内科 affil-num=15 en-affil= kn-affil=重井病院内科 affil-num=16 en-affil= kn-affil=重井病院内科 affil-num=17 en-affil= kn-affil=岡山大学医学部付属病院中央検査部 en-keyword=fulminant hepatitis kn-keyword=fulminant hepatitis en-keyword=viral myocarditis kn-keyword=viral myocarditis en-keyword=catecholamine cardiomyopathy kn-keyword=catecholamine cardiomyopathy END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=415 end-page=423 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of poikilothermia with characteristic electrocardiographical changes during hypothermia kn-title=低体温に特有な心電図変化を示したpoikilothermiaの1例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 60 year old female died suddenly, probably due to arrythmia. At autopsy, cerebral softening from the hypothalamus to the central thalamic nuclei was seen. This was thought to be secondary to a cerebrovascular accident with consequent poikilothermia. During the clinical course, ECG findings characteristic of hypothermia such as sinus bradycardia, bradic atrial fibrillation, prolongation of QT interval, T wave inversion and appearance of J wave (Osborn wave) were noted in association with cold weather. The case is discussed with a review of the literature. en-copyright= kn-copyright= en-aut-name=YoshidaHidenori en-aut-sei=Yoshida en-aut-mei=Hidenori kn-aut-name=吉田英紀 kn-aut-sei=吉田 kn-aut-mei=英紀 aut-affil-num=1 ORCID= en-aut-name=UesugiSeiichiro en-aut-sei=Uesugi en-aut-mei=Seiichiro kn-aut-name=植杉成一郎 kn-aut-sei=植杉 kn-aut-mei=成一郎 aut-affil-num=2 ORCID= en-aut-name=YuasaShiro en-aut-sei=Yuasa en-aut-mei=Shiro kn-aut-name=湯浅志郎 kn-aut-sei=湯浅 kn-aut-mei=志郎 aut-affil-num=3 ORCID= en-aut-name=KusachiShowzo en-aut-sei=Kusachi en-aut-mei=Showzo kn-aut-name=草地省藏 kn-aut-sei=草地 kn-aut-mei=省藏 aut-affil-num=4 ORCID= en-aut-name=SogoTaiji en-aut-sei=Sogo en-aut-mei=Taiji kn-aut-name=十河泰司 kn-aut-sei=十河 kn-aut-mei=泰司 aut-affil-num=5 ORCID= en-aut-name=YamadaNobuyuki en-aut-sei=Yamada en-aut-mei=Nobuyuki kn-aut-name=山田信行 kn-aut-sei=山田 kn-aut-mei=信行 aut-affil-num=6 ORCID= en-aut-name=NishiyamaOsamu en-aut-sei=Nishiyama en-aut-mei=Osamu kn-aut-name=西山修 kn-aut-sei=西山 kn-aut-mei=修 aut-affil-num=7 ORCID= en-aut-name=YasuharaKouichiro en-aut-sei=Yasuhara en-aut-mei=Kouichiro kn-aut-name=安原耕一郎 kn-aut-sei=安原 kn-aut-mei=耕一郎 aut-affil-num=8 ORCID= en-aut-name=UedaMinoru en-aut-sei=Ueda en-aut-mei=Minoru kn-aut-name=上田稔 kn-aut-sei=上田 kn-aut-mei=稔 aut-affil-num=9 ORCID= en-aut-name=SaitoDaiji en-aut-sei=Saito en-aut-mei=Daiji kn-aut-name=斉藤大治 kn-aut-sei=斉藤 kn-aut-mei=大治 aut-affil-num=10 ORCID= en-aut-name=MaeRitsuo en-aut-sei=Mae en-aut-mei=Ritsuo kn-aut-name=前律夫 kn-aut-sei=前 kn-aut-mei=律夫 aut-affil-num=11 ORCID= en-aut-name=ShimadaYoshihiro en-aut-sei=Shimada en-aut-mei=Yoshihiro kn-aut-name=島田宜浩 kn-aut-sei=島田 kn-aut-mei=宜浩 aut-affil-num=12 ORCID= en-aut-name=HaraokaShoichi en-aut-sei=Haraoka en-aut-mei=Shoichi kn-aut-name=原岡昭一 kn-aut-sei=原岡 kn-aut-mei=昭一 aut-affil-num=13 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=6 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=7 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=8 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=9 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=10 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=11 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=12 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=13 en-affil= kn-affil=岡山大学医学部附属病院中央検査部 en-keyword=poikilothermia kn-keyword=poikilothermia en-keyword=J wave (Osborn wave) kn-keyword=J wave (Osborn wave) END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=407 end-page=414 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Malignant transformation of diploid rat liver cells treated with 3'Me-DAB in tissue culture kn-title=単個培養された二倍体性肝細胞の3'-Me-DABによる試験管内癌化 en-subtitle= kn-subtitle= en-abstract=A diploid clone derived from rat liver cells was treated in culture with 3'Me-DAB. The cells treated for 140 days with 5 μg/ml or 80 μg/ml of 3'Me-DAB became malignant. Cells cultured in normal medium after treatment with 3'Me-DAB also became malignant. Untreated control cells did not produce tumors on back-transplantation at the 232nd day stage, when 3'Me-DAB-treated cells were confirmed to be malignant. The transformed cells showed some morphological and chromosomal changes, as well as resistance to the toxic effects of 3'Me-DAB. However, no definite differences in growth potential and serum requirement were found between treated and untreated-control cells. kn-abstract=正二倍体性単個クローンを使用し, 3'-Me-DABによる正常ラット肝細胞癌化の可能性を追求した.1)5γ/mlないしは80γ/ml 3'-Me-DAB,140日間連続処理により,細胞の癌化を認め,以降, 3'-Me-DAB処理後, 3'-Me-DABを含まない正常培地に置き換えられた系でも癌化を認めた.尚対照群では, 3'-Me-DAB処理細胞の殆んどに癌化を確認した時点の232日迄に自然癌化は,認めなかった.2)癌化過程における3'-Me-DAB処理細胞の特性の変化としては,形態異常染色体上の変化, 3'-Me-DABに対する耐性の獲得などが認められたが,増殖性,血清要求性については対照群との差は認められなかった. en-copyright= kn-copyright= en-aut-name=ShiraishiMasayuki en-aut-sei=Shiraishi en-aut-mei=Masayuki kn-aut-name=白石昌之 kn-aut-sei=白石 kn-aut-mei=昌之 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科教室 en-keyword=単個培養 kn-keyword=単個培養 en-keyword=正二倍体性 kn-keyword=正二倍体性 en-keyword=3'-Me-DAB kn-keyword=3'-Me-DAB en-keyword=癌化 kn-keyword=癌化 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=397 end-page=405 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A histochemical study of brown pigment in chromated normal skin and dermatoses kn-title=クロム処理の正常並びに病的皮膚中の褐色顆粒に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The presence of catecholamine containing cells (chromaffin cells) in human skin was deter-mined by fixation with chromate-bichromate solution followd by post-staining and other methods. Some of the brown pigment granules in chromated dermis stained with Giemsa and toluidine blue showed similar staining properties to chromaffin cells in rabbit adrenal medulla treated in the same way. They also were seen in large numbers in nevus pigmentosus, especially in blue nevi, but were rarely seen in various lesions of Recklinghausen's disease nor in atopic dermatitis. Judging from other results, this dermal brown pigment is probably not catechol-amine but melanin or a degradation product of melanin. Other possibilities of the nature of the granules are discussed with special reference to lipid pigment. en-copyright= kn-copyright= en-aut-name=TakaiwaTakashi en-aut-sei=Takaiwa en-aut-mei=Takashi kn-aut-name=高岩堯 kn-aut-sei=高岩 kn-aut-mei=堯 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部皮膚科学教室 en-keyword=クロム親和性反応 kn-keyword=クロム親和性反応 en-keyword=真皮褐色顆粒 kn-keyword=真皮褐色顆粒 en-keyword=メラニン kn-keyword=メラニン en-keyword=リピド色素 kn-keyword=リピド色素 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=379 end-page=396 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Diurnal variations in motor activity and regional brain monoamine content in young adult and aged rats kn-title=若齢成熟および老齢ラットの行動量と脳部位別モノアミン量の日内変動 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Diurnal variations in motor activity and regional monoamine levels of the brain in both young adult and aged wistar male rats were compared. The young adult group (Y group) consisted of 16 week old rats. The aged group (A group) comprised 19 month old rats. All the animals were housed in a room with an alternating 12 hour dark-light cycle. The temperature was kept at 24℃ and the moisture at 55 % . Motor activity was measured in selected rats by Animex. Both groups were then subdivided into six smaller groups. The animals of these groups were sacrificed by decapitation every 4 hours of the 24 hour period (2, 6 and 10 hours in the dark cycle, and 2, 6 and 10 hours in the light cycle; D2, D6, D10, L2, L6 and L10). Immediately after decapitation, brains were collected, dissected into six different regions (amygdala [Amy], corpus striatum [St], cerebral cortex [Cor] , hippocampus [Hip], diencephalon [Die] and brain stem [B. S.]), and the levels of dopamin [DA], norepinephrine [NE] and serotonin [5-HT] in each determined fluorometrically. The results were as follows. 1. Diurnal variation of motor activity. Both Y and A groups showed a marked difference between the dark and light cycles. Activity increased during the first 4 hours of the dark cycle, then decreased gradually during the rest of the dark cycle. Motor activity decreased markedly immediately after beginning of the light cycle, and remained decreased throughout the light period. The motor activity of the both groups increased transiently a little before the light cycle. Y group activity increased directly before the dark time. A group activity was significantly less than that of Y group, except for 2 time points during the light cycle. A group motor activity was about 50 % lower than that of the Y group. 2. Diurnal variation of DA. Amy from both groups showed a higher levels in the dark time than in the light. St showed two peaks in both groups but the range of variation was wider in A group than in Y group. Cor from both groups showed a higher level in the light time than in the dark. The levels of Hip, Die and B. S. in both groups showed little variation, the range being much smaller in A group. There was no significant difference in the levels of Amy, Cor and Hip in either group, but St, Die and B. S. were significantly lower in A group throughout almost the entire 24 hour period. 3. Diurnal variation of NE levels. Marked variations in Amy, Cor, Hip and Die occurred in both groups. The NE level in the 4 brain regions tended to be lower during the dark time than during the light. The diurnal variation of amine levels in St was smaller in the groups than in the other five regions. B. S. in the Y group showed lower levels during the dark time; however, B. S. from A group showed almost constant levels during the 24 hour period. The NE levels of each groups showed little difference in brain regions other than B. S.. In A group, B. S. showed 1.8 to 9 times higher levels than Y group at all examined time points. 4. Diurnal variation in 5-HT levels. Both groups showed higher amine levels in Amy, Hip and Die during the dark period than in the light. St in Y group showed two peaks at D10 and L6 but the same region in A group showed almost constant amine levels during the diurnal period except for D6. The level in Cor from both groups showed little variation, the range being much smaller in A group than in Y group. The level in B.S. of Y group showed a marked dark-light difference with a high peak at L2 but the same region from A group showed a gradual variation in the amine level with a peak between D10 and L2. Comparison of the 5-HT levels in Y and A groups showed that there was no significant difference in Amy, Hip and Die. The level in St remained high throughout the diurnal period in Y group. The level in Cor showed a higher value during the 24 h period in A group. The amine level in B. S. was higher in the A group at two time points of the dark period than in Y group. The data indicate a characteristic influence of aging on diurnal variations of motor activity and three monoamine contents of the brain. Some regions of the brain showed no difference in DA levels of Y and A groups, whereas other regions showed significantly lower levels in A group. Some regions showed no difference in NE levels of both groups and the other showed significantly higher levels in A group. These results suggest that the turnover rate of DA in some brain regions is accelerated in A group compared to Y group. A group tended to show less diurnal variation in the three monoamine levels of the brain than Y group when related to the reduced motor activity of A group, this suggests that metabolism of these monoamines in related to behavior. en-copyright= kn-copyright= en-aut-name=DoiTohru en-aut-sei=Doi en-aut-mei=Tohru kn-aut-name=土井亨 kn-aut-sei=土井 kn-aut-mei=亨 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳代謝研究施設脳代謝神経科 en-keyword=老化 kn-keyword=老化 en-keyword=日内変動 kn-keyword=日内変動 en-keyword=運動量 kn-keyword=運動量 en-keyword=脳内モノアミン量 kn-keyword=脳内モノアミン量 en-keyword=部位別脳 kn-keyword=部位別脳 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=355 end-page=378 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of extracorporeal circulation on myocardial metabolism and postoperative cardiac function kn-title=開心術中の心筋代謝と術後心機能に関する臨床的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The experiment was carried out to investigate myocardial metabolism during extracorporeal circulation (ECC) and cardiac function following open heart surgery. Myocardial changes were assessed by measuring the levels of cyclic AMP, ATP, lactate and pyruvate in the right atrial myocardium and coronary blood. Myocardial cyclic AMP and ATP decreased significantly during ECC, although they remained with moderate hypothermia. The level of lactate in the myocardium rose in all cases. There was no significant correlation between ECC time and hypothermia. Serum lactate was elevated at the beginning of ECC and increased progressively during ECC, but the coronary A-V difference of lactate (⊿L) remained unchanged. The serum pyruvate level increased moderately during ECC but its A-V difference did not vary. The data suggest that lactate and pyruvate were taken up by the myocardium. There was a significant inverse correlation between cardiac index and ECC time at 2, 4 and 6 hours after operation. Stroke index showed attitude similar to cardiac index. There was a significant positive correlation between total peripheral vascular resistance and ECC time. The cardiac index and total peripheral vascular resistance with hypothermia were significantly better than those with normothermia. Patients with higher myocardial cyclic AMP and ATP at the end of ECC had better cardiac indices at 2 and 6 hours after open heart surgery. en-copyright= kn-copyright= en-aut-name=HaraKouhei en-aut-sei=Hara en-aut-mei=Kouhei kn-aut-name=原浩平 kn-aut-sei=原 kn-aut-mei=浩平 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二外科教室 en-keyword=体外循環 kn-keyword=体外循環 en-keyword=開心術後心機能 kn-keyword=開心術後心機能 en-keyword=心筋代謝 kn-keyword=心筋代謝 en-keyword=サイクリックAMP kn-keyword=サイクリックAMP en-keyword=ATP kn-keyword=ATP END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=335 end-page=353 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Magnesium metabolism during total parenteral nutrition in rabbits kn-title=完全静脈栄養におけるMg代謝に関する実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Magnesium metabolism was studied during total parenteral nutrition (TPN) in rabbit. Rabbits were fed for 14 to 17 days by TPN of 110 Cal/kg/day with different doses of magnesium. Results were as follws: 1. Excretion of magnesium during starvation was 1.5 mEq/kg/day. The urinary Mg/N excretion ratio was 3.4 mEq/Ng. 2. An intake of 2.5 mEq/kg/day of magnesium was required to maintain normal plasma and erythrocyte magnesium levels, plasma P and Ca should be supplied at the rate of 9 mEq/kg/day (8.2 mEq/100Cal) and 1 mEq/kg/day respectively. 3. The excretion of magnesium, phosphorus and potassium increased during low calorie parenteral nutrition. 4. Although no depletion of nitrogen balance and body weight occurred during TPN with-out magnesium, impairment of K, P and Ca utilization was suspected. 5. This study of magnesium balance showed that magnesium may be required at early stages of TPN. 6. Magnesium deficiency was diagnosed by a fall in the levels of plasma magnesium, erythrocyte magnesium, and urinary magnesium excretion. This experiment indicates that TPN which includes quantities of magnesium may be needed for the metabolism of K, P and Ca. en-copyright= kn-copyright= en-aut-name=NoguchiAtsushi en-aut-sei=Noguchi en-aut-mei=Atsushi kn-aut-name=野口敦 kn-aut-sei=野口 kn-aut-mei=敦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二外科学教室 en-keyword=Mg代謝 kn-keyword=Mg代謝 en-keyword=完全静脈栄養 kn-keyword=完全静脈栄養 en-keyword=nitrogen balance kn-keyword=nitrogen balance END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=313 end-page=334 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Scanning electron microscope study of cerebral ventricular wall of hamster kn-title=脳室壁の走査電子顕微鏡像 ―部位と発育に伴う表層構造の相異― en-subtitle= kn-subtitle= en-abstract= kn-abstract=The normal cerebral ventricular surfaces of the hamster at all ages were studied using a scanning electron microscope with special emphasis on the development of cilia in relation to the location of ventricle and developmental stages. 1) Considerable variations of cilia were observed between individual cases even in the same developmental stages and the same ventricle. However, in general, the distribution of ciliated cells was dense on fourth and less on lateral ventricles, and the development of cilia was most advanced in fourth ventricle. 2) I the early postnatal stage, single cilium or multiple cilia of 1μ in length were seen on the surface of ependymal cells, and several rudimentary cilia were observed in places. 3) In the later embryonic stage, cilia of 1μ in length were found on the domed tip of ependymal cells of the fourth ventricle, and numerous bleb-like protrusions were observed on the surface of ventricles. 4) Fully developed cilia of 10μ in length and microvilli were recognized about 3 to 4 weeks after birth. The cilia and microvilli decreased in number as the animal age increased. 5) It is suggested that the differentiation of the surface organelles of ependymal cells may be controlled by physiological function and the dynamics of cerebrospinal fluid. en-copyright= kn-copyright= en-aut-name=IkuboTsutomu en-aut-sei=Ikubo en-aut-mei=Tsutomu kn-aut-name=井久保勤 kn-aut-sei=井久保 kn-aut-mei=勤 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部病理学教室 en-keyword=線毛 kn-keyword=線毛 en-keyword=脳室表面 kn-keyword=脳室表面 en-keyword=走査電子顕微鏡 kn-keyword=走査電子顕微鏡 en-keyword=ハムスター kn-keyword=ハムスター END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=281 end-page=312 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental studies on the transplantation of developing teeth kn-title=未完成歯移植に関する実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To correct esthetically a defect of a tooth and to ensure the recovery of its function, tooth transplantation could be usefully employed. The author studied how the transplanted tooth that had been once deprived of its nutrition could have the blood vessels in the dental pulp as well as in the apical membrane regenerate and function, and further compared its primary adhesion according to the presence or absence of the apical membrane. For this purpose juvenile dogs were used, and changes in the blood vessels were investigated with specimens prepared by the method of chloropercha injection into the blood vessel, and then morphological and three-dimensional observations were carried out, and further radiological, macroscopic as well as histopathologic observations were conducted, and the results are summarised as follows. 1) Newly regenerated blood vessels in the dental pulp of the transplanted teeth on B side (the group not deprived of apical periodontal membrane) began to develop in the dental pulp of the apex from about the third postoperative day, and by the thirtieth day the regenerated vessels had formed up to the crown and thereafter blood circulation became uniform. On A side (the group deprived of its apical periodontal membrane) the recovery of the intramedullar blood vessels was delayed in comparison with that on B side and the blood vessels developed in the dental pulp by the fifth postoperative day and by the sixtieth day the newly regenerated vessels were observed running up to the dental pulp of the crown. 2) Histopathologically, on B side the dental pulp underwent post-transplantation degeneration and necrosis, but by the third postoperative day granulous tissue infiltrated from the apex and by the thirtieth day the dental pulp was almost completely replaced by connective tissue. On A side changes similar to B side were observed but the proliferation of the granulation tissue was delayed by two days. As for the disrupted periodontal membrane on B side recovery by the regeneration of connective tissue from the third postoperative day, while on A side there occurred an osteoid fusion from about the twentieth day. 3) For the primary adhesion of the transplanted tooth the apical periodontal membrane played an important role. 4) Radiologically, up to the seventh postoperative day a pathological gap was observed, while on B side the formation of the dental apex from the tenth day which was completed by the thirtieth day was observed. On A side absorption of the apex of the root was completed by the thirtieth day. 5) Macroscopically, on both A and B sides swelling and reddening of the gingiva was observed. On B side the dentition could be seen by the thirtieth day, and the bone to which the tooth was transplanted became firm by the sixtieth day and thereafter. 6) A certain correlation in the changes of blood vessels, X-ray findings, macroscopical, and pathohistological findings was evident. en-copyright= kn-copyright= en-aut-name=MaedaKenichiro en-aut-sei=Maeda en-aut-mei=Kenichiro kn-aut-name=前田健一郎 kn-aut-sei=前田 kn-aut-mei=健一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部口腔外科学教室 en-keyword=未完成歯移移植 kn-keyword=未完成歯移移植 en-keyword=血管新生 kn-keyword=血管新生 en-keyword=歯根膜保存 kn-keyword=歯根膜保存 en-keyword=歯根膜除去 kn-keyword=歯根膜除去 en-keyword=骨性癒着 kn-keyword=骨性癒着 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=261 end-page=279 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Biological effects of 2,450 MHz microwave radiation on rat glioma cells in vitro kn-title=培養ラットグリオーマ細胞(C6)に対するマイクロウエーブ照射の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A monolayer culture of a clonal cell strain of rat glioma was exposed to 2,450 MHz microwave (continuous wave) radiation. The clonal cell strain was the C6 strain established by Benda, et al. (22). The temperature of the medium was measured with a thermistor probe placed under each culture bottle, and it was maintained at 37℃ or at 38.5℃ during 10-hour irradiation, and similarly at 40℃ during 5-hour irradiation. After each irradiation, the culture bottle was incubated at 37℃ for 48 hours. The cell growth of C6 cells exposed to microwave radiation for 10 hours did not show any changes as long as the temperature of the medium was kept lower than 38.5℃. However, when the temperature was kept at 40℃ for 5 hours by microwave irradiation, C6 cells significantly decreased (63%) in numbers on the 2nd day after the treatment (Table 1). Ten days later, these cells resumed their exponential growth and their population doubling time was same as that of nontreated C6 cells (Fig. 9). On the other hand, the treatment of the cells at 40℃ for 5 hours in a conventional incubator resulted in only a 20% decrease in cell numbers and 10-hour treatment at 41.5℃ resulted in a 48 % decrease in the number of C6 cells (Fig. 9). The difference between the effects on cell numbers of microwave radiation and conventional heat incubation suggest that the temperature distribution within the culture bottle might not be uniform under microwave irradiation. Therefore growth inhibition of the cells after hyperthermic (40℃) microwave irradiation was considered probably due to the thermal effects rather than to the nonthermal effects of electromagnetic irradiation. After microwave irradiation, May-Giemsa staining of surviving cells did not reveal any morphological abnormalities (Fig. 6). Feulgen reaction of the cells did not reveal any changes in the number of nuclear DNA particles, either (Fig. 7). In addition, chromosome analysis, performed on the 2nd and the 10th day after microwave radiation, did not show any appreciable chromosome damage. One hundred metaphases of C6 cells were examined for each analysis and 84% of them had the stemline number of 40 chromosomes (Table 2). The karyotype of these cells was not different from that of nontreated C6 cells. In the stemline karyotype, five chromosomes were missing: one chromosome of the A2 pair, one chromosome of the A3 pair, one chromosome of the C12 pair, and two chromosomes from group B. There were also constant gains of three markers (Fig. 8). The present results indicate that 2,450 MHz continuous wave radiation on the C6 cells did not reveal any nonthermal effects of electromagnetic radiation, in contrast to several other reports: Webb and Dodds (47), Yao, et al. (48), Okai (50), Webb and Booth (59), and Heller (67). en-copyright= kn-copyright= en-aut-name=HaradaYasuhiro en-aut-sei=Harada en-aut-mei=Yasuhiro kn-aut-name=原田泰弘 kn-aut-sei=原田 kn-aut-mei=泰弘 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科学教室 en-keyword=マイクロウエーブ kn-keyword=マイクロウエーブ en-keyword=生物学的作用 kn-keyword=生物学的作用 en-keyword=C6ラットグリオーマ細胞 kn-keyword=C6ラットグリオーマ細胞 en-keyword=組織培養 kn-keyword=組織培養 END