start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=223 end-page=229 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cytotoxic effect of sensitized apleen lymphocytes with SV40 and SV40 induced tumor on SV40 transformed cells kn-title=SV40ウイルス及びSV40誘発腫瘍細胞により感作されたリンパ球のSV40変異ヒト胎児細胞に対する細胞障碍作用 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sensitized lymphocytes have been demonstrated to inhibit tumor growth in vivo systems. In SV40-viral oncogenesis, some specified immunization with virns and with virus-induced tumors to the animals results in rejection of further transplantation of the tumor cells. To analyze these phenomena by our specially devised cytotoxicity test, the authors have studied cell mediated immunity for tumor specific transplantation antigen (TSTA) in SV40 viral oncogenesis. The results suggest that sensitized lymphocytes inhibit the growth of heterogenic SV40-transformed target cells as well as syngenic ones. And the lymphocytes from tumor-bearing animals have lower ability of the inhibition on the target cells, which may be explained that the lymphocytes in tumor bearing animals are under the condition of immunologically insufficient state. en-copyright= kn-copyright= en-aut-name=EgusaKuniyuki en-aut-sei=Egusa en-aut-mei=Kuniyuki kn-aut-name=江草国之 kn-aut-sei=江草 kn-aut-mei=国之 aut-affil-num=1 ORCID= en-aut-name=SatoKeiko en-aut-sei=Sato en-aut-mei=Keiko kn-aut-name=佐藤啓子 kn-aut-sei=佐藤 kn-aut-mei=啓子 aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name=小田琢三 kn-aut-sei=小田 kn-aut-mei=琢三 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌研生化学 affil-num=2 en-affil= kn-affil=岡山大学医学部癌研生化学 affil-num=3 en-affil= kn-affil=岡山大学医学部癌研生化学 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=211 end-page=221 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Basophilic Leucocytes Part. V. Changes of Basophilic Leucocytes in Chronic Myelogenous Leukemia kn-title=好塩基球に関する研究 第3編 慢性骨髄性白血病における好塩基球の動態に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Basophils in peripheral blood and bone marrow in 25 cases with chronic myelogenous leukemia (CML) were counted and morphologically classified by our improved method; basophilis were classified into T to X types. The V and W types were subclassified to S type with sharply defined granules and diffuse N type. This classification was adapted for basophilograms. The basophilogram in healthy controls showed a left type and that in CML generally a right type. It shifted to the left approaching to the normal type in the cases with remission by chemotherapy. In relapsing patients with CML, the basophilogram clearly shifted to the right. The basophilogram seen in 2 cases with the pre-stage of blastic crisis of CML indicated a definite right type. It was shown that an increase of S type occurred in the relapsing stage. Therefore, appearance of S type as well as a right type in basophilogram would be a useful clue to follow CML patients clinically. A basophils of S type was considered to be immature. The bone marrow basophilogram was shown to be more shifted to the right than that of peripheral blood, and this tendency became more remarkable in relapse. Presence of a right type in basophilogram and appearance of S type are significant to observe the clinical course in CML patients, especially for judging acute exacerbation. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=199 end-page=210 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Basophilic Leucocytes Part. 11. Changes of Basophilic Leucocytes in Bronchial Asthma kn-title=好塩基球に関する研究 第2編 気管支喘息における好塩基球の動態に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using mainly our improved method of Kovacs, Changes of basophilic leucocytes in peripheral blood were observed in 181 patients with bronchial asthma and 20 patients with allergic occupational asthma caused by inhaling the body fluid of sea spuirts attaching to the shell of cultured Oyster. This study shows that basophils have a strict relationship to asthmatic attacks ; the basophils show a normal level in non-attack stage as in healthy controls, an increase in pre-attack, and tend to decrease in attack. A close observation on changes in the numbers of peripheral basophils almost make an early detection of the forth-coming attack possible. The threshold of asthmatic attack indicated by basophil count, 65/cmm, is very significant for the observation of forth-coming asthmatic attack, and this threshold of asthmatic attack varied, in some extent, though constant, in each case. These changes of basophils were similar in the cases given corticosteroids. In eosinophils changes were not so clear-cut. Concerning a prolonged therapeutic course, basophils in bronchial asthma were over the threshold in the stage of repeated asthmatic attacks, and lower in the non-attack stage. Patients with allergic occupational asthma showed these similar changes. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=189 end-page=197 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Basophilic Leucocytes Part. T A Improved Method for a Simple Direct Method Counting Absolute Basophils and Eosinophils and its Clinical Significance kn-title=好塩基球に関する研究 第1編 好塩基球および好酸球の同時直接算定法の考案とその臨床的評価 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Improved methods were made to count the absolute numbers of basophils and eosinophils by adding phosphate buffer to Kovacs staining fluid. The improved method has following advantages. 1) Both basophil and eosinophil leucocytes can be easily recognized and identified simultaneously. 2) The staining fluid is stable for more than one year at room temperature. 3) Within two hours after filling a pipette, precipittates or ghost cells still do not appear. 4) This method is simple and convenient for the routine examination like calculation of peripheral leucocytes. Changes of basophil leucocytes in the peripheral blood in various diseases were investigated with above described method. Basophilia was shown in allergic rhinitis, bronchial asthma, parasite infection, idiopathic thrombocytopenic purpura, acquired hemolytic anemia, hypothyroidism, iron deficiency anemia, chronic myelogenous leukemia and Addisons disease. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=183 end-page=187 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on T-antigen of the clones derived from a single cell in SV 40-transformed mouse embryo cells by the immunofluorescent method kn-title=SV40変異マウス胎児細胞のSingle Cell由来CloneのT抗原の消長―螢光抗体法による解析― en-subtitle= kn-subtitle= en-abstract= kn-abstract=In our previous observation, the T-antigen in unclond mouse embryo cells transformed by SV 40 or SV 40 DNA declined gradually through long term cultivation. To analyze whether this phenomenon is due to selection or reversion and/or conversion, we observed the T-antigen of a series of clones derived from a single cell in the SV 40-transformed cells by the immunofluorescent method. The T-antigen was either positive or negative in all cells in each clone, and none of the clones was consisted of the mixture of T-antigen positive and negative cells. The T-antigen negative cells were more predominant in the rate of growth than the T-antigen positive ones in this mouse embryo cells. One reasonable explanation on the data may be that the decline of T-antigen in the uncloned SV 40-transformed cells is due to the selection of the T-antigen negative cells. This fact may be one of the reason for difficulty in establishing the SV 40-transformed mouse embryo cell lines. en-copyright= kn-copyright= en-aut-name=EgusaKuniyuki en-aut-sei=Egusa en-aut-mei=Kuniyuki kn-aut-name=江草国之 kn-aut-sei=江草 kn-aut-mei=国之 aut-affil-num=1 ORCID= en-aut-name=SekiShuji en-aut-sei=Seki en-aut-mei=Shuji kn-aut-name=関周二 kn-aut-sei=関 kn-aut-mei=周二 aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name=小田琢三 kn-aut-sei=小田 kn-aut-mei=琢三 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌研生化学 affil-num=2 en-affil= kn-affil=岡山大学医学部癌研生化学 affil-num=3 en-affil= kn-affil=岡山大学医学部癌研生化学 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=165 end-page=182 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of Actinomycin D on the Central Nervous System of Higher Animals kn-title=Actinomycin Dの高等動物中枢神経系に対する作用 en-subtitle= kn-subtitle= en-abstract=When a dose of actinomycin D was intrathecally injected into higher animals (dogs and cats), these animals experienced generalized seizures after a latent period of several days. The critical seizure producing quantity was 0.025mg on the dog. Actinomycin D combines with DNA in the nervous tissue of the brain, this may be the cause of the seizure effect. When the same dose (0.025mg) of actinomycinic acid, which has a chemical structure resembling that of actinomycin D, but does not combine with DNA in the tissue, was injected into the cerebro-spinal fluid of the dog, the animals did not show any behavioural change. Electroencephalographic studies of the actinomycin D seizure were carried out on the cat. Several days after the administration of actinomycin D, the animal showed the typical generalized seizure discharges. A generalized seizure discharge induced by actinomycin D began with the occurrence of frequent spikes in group in all of recording sites, then the repetition of a spike and wave complex or a polyspike and wave complex followed. The intervals of each spike and wave complex gradually grew longer, and the seizure discharge stopped. After the actinomycin D administration, the electroencephalogram sometimes showed remarkable spike discharges occurring in the hippocampal region. They occurred mostly in hippocampus, but to a lesser degree spike discharges occurred in the other regions. In the preconvulsive state after the actinomycin D administration, regular waves of 8 cps occasionally appeared simultaneously with the occurrence of myoclonic jerks. In the case of the injection of actinomycinic acid into the cat, no significant change occurred on the electroencephalogram. However, the injection of DNA into the cerebrospinal fluid, simultaneously performed, with the injection of actinomycin D, did not stop the seizure effect of the latter. Methionine sulfoximine, characterized by a seizure effect with a long latent period, was also injected into the cerebro-spinal fluid of the dog. The critical seizure producing quantity was 0.18mg. These two convulsants with long latent period, the actinomycin D and the methionine sulfoximine, did not produce seizure effects when combined in different proportions. Three butyric acid derivatives (GABA, GABOB and S-GABA) did not arrest the actinomycin D seizure effect. kn-abstract=1.Actinomycin Dをイヌの髄液内に投与すると,3〜5日の長い潜時で動物は全身痙攣を起こした.この痙攣量は0.025mgであり,それによって42例中33例に痙攣が見られ,その平均潜時は89.5±10時間であった.痙攣型は定型的な全身痙攣であり,動物は激しく痙攣を繰返したのち全例死に至った.2.Actinomycinic acidを髄液内に投与しても痙攣は認められなかった.3.ネコの髄液内にactinomycin Dを投与して数日後に,動物は脳波上定型的なgeneralized seizure dischargeを反復して示した.この場合まず高振幅spikeの群発が起こり,これにspike and waveまたはpolyspike and wave complexがつづいた.やがてspike and wave complexの出現頻度がしだいに減少し,発作波が終了した.またactinomycin D投与により, hippocampusに顕著なspike dischargeが見られることがあった.また痙攣前駆期において,myoclonic jerkの発生と同時に,hippocampusを除く全誘導に8cpsの規則的な波が見られた.他方,actinomycinic acidの投与によっては,脳波上にも顕著な変化はあらわれなかった.4.Actinomycin Dと同時にDNAを髄液内に投与し,その拮抗作用の有無をしらべたが,このDNAの投与によっては, actinomycin D痙攣は防止できなかった.5. Actinomycin Dとmethionine sulfoximineとの干渉作用を検討し,痙攣閾量以下のmethionine sulfoximineはactinomycin Dの痙攣作用を妨げないが,逆に痙攣量の半量付近のactinomycin Dはmethionine sulfoximineの痙攣作用を抑制することを観察した.6. GABA,GABOB,S-GABAのactinomycin D痙攣に対する確実な抑制作用は,ほとんど認められなかった. en-copyright= kn-copyright= en-aut-name=TairaMitsuo en-aut-sei=Taira en-aut-mei=Mitsuo kn-aut-name=平光雄 kn-aut-sei=平 kn-aut-mei=光雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳代謝研究施設機能生化学部門 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=161 end-page=164 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Absorptions spectra of indirect and direct bilirubin kn-title=間接bilirubinと直接bilirubinのAzo色素の吸収曲線 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Direct bilirubins in vivo was fractionated into bilirubin diglucuronide, bilirubin phosphate and bilirubin sulfate. Influence of pH concentration on the absorptions maximum of each azopigment was studied. The schift of absorptions maximum was the same in the former two direct bilirubins and the latter moved as nearly as the indirect bilirubin-azo pigment yielded after an addition of methanol. These results was supposed to the difference was based on the structual difference of bilirubin due to the conjugation type of bilirubin. en-copyright= kn-copyright= en-aut-name=KondoTadasuke en-aut-sei=Kondo en-aut-mei=Tadasuke kn-aut-name=近藤忠亮 kn-aut-sei=近藤 kn-aut-mei=忠亮 aut-affil-num=1 ORCID= en-aut-name=HirohataMamoru en-aut-sei=Hirohata en-aut-mei=Mamoru kn-aut-name=広畑衛 kn-aut-sei=広畑 kn-aut-mei=衛 aut-affil-num=2 ORCID= en-aut-name=SeoKenji en-aut-sei=Seo en-aut-mei=Kenji kn-aut-name=瀬尾憲司 kn-aut-sei=瀬尾 kn-aut-mei=憲司 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第一内科教室 END