岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 抗リンパ球血清（ALS）の作用機作ならびに力価判定法に関する実験的研究 151 159 EN Katsutoshi Shinoda After injecting mouse lymph node cells added with adjuvant to rabbits by i. v. or i. m. route, 13 groups of anti-lymphocyte sera (ALS) were made. Then there were measured the effect of ALS in vitro on the blastformation rate in one-way mixed lymphocyte cultures (MLC) in addition of PHA and the lymphocytotoxic titer of ALS. In this study, observations were also carried out to determine the blastformation rate of responding lymph node cells obtained from ALS injected mice in MLC with PHA, the PHA-induced cytotoxic effect of lymphnode cells described just above and the effect of administration of ALS on the survival time of skin allografts in mice. The results of the study are briefly summarized as follows. 1. ALS in vitro suppress the blastformation rate in the MLC of mouse lymph node cells in the presence of PHA. 2. ALS in vitro inhibit cytotoxic effect of mouse lymph node cells on target allogeneic cells in the presence of PHA. 3. ALS in vitro suppress the blastformation rate of mouse lymph node cells in the MLC added with PHA, and also ALS that inhibit the PHA-induced cytotoxic effect on the target allogeneic cells, prolong the survival time of mouse skin allograft. 4. The lymphocytotoxic titer of ALS in vitro is not correlated to the effect of ALS administration on the survival time of mouse skin allograft. 5. It has been demonstrated that ALS prepared by i. v. route with adjuvant are generally stronger than ALS prepared by i. m. route in those inhibitory effect on transplantation immunity, on the rate of blastformation of MLC added with PHA and on the PHA-induced cytotoxic effect of lymph node cells. 6. Inhibitory effects of ALS on transplantation immunity have better correlation with the decreasing levels in blastformation rate of MLC following addition of these ALS and the decreasing degrees of the PHA-induced cytotoxic effect by addition of these ALS than with these lymphocytotoxic titers. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 ラット臓器の脂質過酸化反応におよぼす放射線照射の影響 第3編 放射線照射ラツト各臓器の脂質過酸化物形成能に及ぼす放射線防護物質投与(in vivo)の影響について 145 150 EN Setsuo Watanabe Effects of tathione and cepharanthin, the radiation-protective agents, on the lipid peroxidation in rat organs were studied, and obtained the results as follows. (1) The acceleration of lipid peroxide formation induced by X-irradiation can be inhibited by the administration of tathione prior to the irradiation and by the dose administered. Such an inhibitory effect is especially marked in the liver, and as reported in Part �T and Part �U, in view of correlations among X-irradiation effect, lipid peroxidation and fatty acid composition in the liver, it seems that glutathione in vivo is somehow involved in the acceleration of lipid peroxidation. (2) The increase of lipid content observable in the liver of X-irradiated animal could not be observed in the animals administered with tathione. (3) Cepharanthin showed not any relation to the lipid peroxidation. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 ラット臓器の脂質過酸化反応におよぼす放射線照射の影響 第2編 放射線照射ラット臓器における脂質過酸化反応と脂肪酸構成比の変動について 137 143 EN Setsuo Watanabe With four groups of rats, normal, irradiated, fasting, and irradiated-then-fasting rats (dose of X-ray, 650R), 48 hours afterwards, liver, heart, spleen and kidney were examined separately as to changes in the acceleration of lipid peroxidation and fatty acids due to irradiation were examined and obtained the results as follows. (1) The lipid peroxidation in each organ was accelerated after irradiation when compared to that in the organs of normal group. In the fasting group the acceleration of lipid peroxidation was observed, but the acceleration in the irradiated group was far more marked, indicating clearly the effect of irradiation. (2) As to changes in the ratio of fatty acid composition in every organ of the irradiated group there were observed an increase in the ratio of high unsaturated fatty acids and a decrease in the ratio of saturated fatty acids as compared with the respective values in normal group. Among organs there could be recognized no uniform change in the fasting group and the irradiated-then-fasting group. These results indicate that the fasting affects the ratio of fatty acid composition, especially marked was change in triglyceride fraction. (3) The lipid content in the liver tends to increase after irradiation, and there is also observed an incrase in the ratio of lipids to phospholipids. (4) The arachidonic acid of phospholipid what is considered to be substrate of lipid peroxidation is increased in the irradiated group, suggesting its relation to the acceleration of lipid peroxidation accompanying irradiation, and actually, the arachidonic acid ratio of phospholipid is markedly decreased after the incubation of lipid peroxidation reaction in the presence of Fe(++), indicating that this substance can play the role of substrate. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 ラット臓器の脂質過酸化反応におよぼす放射線照射の影響 第1編 放射線全身照射ラット臓器の脂質過酸化物形成能について 129 136 EN Setsuo Watanabe After the whole body X-irradiation (650R) of rats, the lipid peroxide formation of various organs were estimated by the TBA reaction, and the results are presented as follows: (1) In TBA reaction tests on the lipid peroxidation in the liver, heart, spleen and kidney with homogenates being incubated, the TBA value were slightly high with endogenous homogenates, but on the addition of Fe(++) the reaction was further accelerated markedly. With liver and kidney that showed a high reaction on addition of Fe(++), the reaction was similarly accelerated on addition of ascorbic acid, but with heart and spleen the reaction at initial stage did not show any significant difference from that of the group without any addition. (2) In every organ from the irradiated animal the activity of lipid peroxide formation was greater than that in organs from non-irradiated control animals. (3) TBA value in the group added with Fe(++) were higher than those in the group without any addition, suggesting that X-irradiation greatly the lipid peroxidation in organs. (4) Change in the acceleration of lipid peroxidation accompanying the irradiation appears as a shortening of reaction-induction time. (5) Radioactivity-dose dependent change can be observed only in the liver, and the time-lapse change after irradiation can also observed only in the liver. (6) These results suggest that the lipid peroxidation following the whole body X-irradiation shows a significant change primarily in the liver. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 妊婦血中 Gestagen 5分画の新測定法に関する研究 115 127 EN Noboru Kawanobe With recent advance in analytical techniques many reports have appeared on the assays of steroid hormones, but as to the blood progestin at present the majority of these reports are concerned only with progesterone from the same test specimen, and there is as yet no report on simultaneous assays of other progestins. Therefore, the author has designed an analytical method for analyzing simultaneously 5 fractions of blood progestin. This method consist of 1) deproteinization, 2) the extraction of steroids, 3) delipidization, 4) alumina column chromatography, 5) the impregnant thin layer chromatography with ethyleneglycol, followed by the color assay, and the results of study on the turnover rate at each step have given satisfactory findings. As for the identificative analysis; 1) analysis by color reaction, 2) gas-liquid chromatography as well as 3) by thin layer chromatography, all yielded satisfactory results. Using this method, the author measured blood progestins at the first, the second and the third trimester of pregnancy. The results have demonstrated that there are many cases where the measurement of 17α-OH-progesterone is not possible because of its minimal quantity, but the other four fractions all showed an increasing tendency along with the advance in pregnancy stage. However, there could be observed no clear-cut correlations between the concentration of these progestin fractions and the uterine muscle susceptibility to oxytocin, neither was there any correlation between the fraction ratios centering around Δ5-3β-ol-steroid dehydrogenase and isomerase and the fraction ratios centering around 20-reductase. It has been suggested that the study of the states in the uterine muscular layer needs to be carried out simultaneously with the study of blood progestin in future. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 Differential Hypothermia下における脳血管透過性亢進に関する研究 103 113 EN Ken Suga Though many laboratory and clinical trials of tumor heating or cooling have been made for the treatment of intracranial tumors, the effects have been reported to be incomplete and unsatisfactory in most of the cases. In 1965, Popovic et al. reported that differential hypothermia, to keep an experimental tumor normothermic under general body hypothermia, showed more marked effect on the tumor in experimental animals. In the present study, the author investigated the uptake of intravenously administered RISA and sodium fluorescein to the brain tissues of mongrel dogs, in which cold induced lesions were made by a stick of dry ice bilaterally on the cerebral cortices. RISA was administered to those dogs intravenously 3 to 4 hours after making the lesions, while sodium fluorescein 30 to 40 minutes prior to the end of the D.H. treatment, respectively. Under total hypothermia (rectal temperature: 21-23℃), the D.H. treatment was performed for 5 hours. It was found that the uptake of RISA through the cerebral blood vessels was increased in and around the cortical lesions. This change was observed only during the D.H. treatment, and disappeared after the body temperature returned to the normal level. In the observation of sodium fluorescein at the tissue level increased permeabiliity of blood vessels was also observed. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 欠損SV40ウイルスDNAの複製形と試験管内発癌 87 101 EN Shinichiro Yamamoto Successive undiluted passages of SV40 (small plaque type) in VERO cells result in the production of heterogeneous defective particles of lower infectivity than that of infectious virions. It was certified that the infectivity of SV40 ran parallel with the length of its DNA. Thus the defectiveness of virions is due to the decrease in the amount of DNA contained in the virions. Replicating circular molecules of SV40 DNA were isolated from SV40 infected VERO cells. Preparations from dilute and undiluted passaged SV40 virus stocks were shown to give different frequency distributions of contour length of replicating DNA molecules (RF-DNA). The mean length of RF-DNA was 1.26±0.21μ and 1.57±0.81μ for the undiluted and dilute passaged SV40 stocks, respectively. Both types of replicating molecules (θ formand σ form) were noted in both stocks. Small replicating DNA molecules with a contour length less than 1.0μ were noted only in undiluted passaged virus stocks. The presence of shortened replicating forms of viral DNA in undiluted passaged virus stocks would indicate that the DNA of defective SV40 virus can replicate. The DNA isolated from undiluted SV40 virus stocks can transform mouse embryo cells in the presence of 300μg/ml diethylaminoethyl-dextran (DEAE-dextran). T antigen induction was predominant at 5 days after infection, but decreased with increasing time until 30 to 50 days after infection. Numbers of T antigen positive cells increased up to 30% again with morphological changes accompanied. The transformed mouse cells showed criss-cross appearance, pleomorphic nuclei with many nucleolei and irregurality of cell arrangements. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 鉛微粒子吸入によるラッテ体内の鉛の分布 第二報 81 85 EN Kazuo Kumashiro 1) The distribution in the organs of Wister rats (female) was studied by making them inhale lead fume for four hours, the concentration of which was as high as 15.2 mg/m(3). 2) Immediately after inhaleling lead fume, the amount of distributed lead increased clearly in lung, liver and kidney, and slightly increased in spleen, bone marrow and bone, and after seven days amount in blood of inhaled rat came to the normal level. 3) It is inferred that kidney and bone are the organ where lead accumulates. 4) It was recognized that polychromatic red cells increased in the peripheral blood. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 Bleomycinの造血器に及ぼす影響に関する研究 第3編 Bleomycinによる造血器腫瘍の治療 71 79 EN Ikuya Kunimasa Bleomycin was administered to 14 patients with malignant lymphoma (5 cases of reticulum cell sarcoma, 6 cases of Hodgkin's disease, 1 case of lymphosarcoma and 2 cases of histologically unclassifed type), and also given to 9 patients with leukemia. In 10 of the 12 cases of malignant lymphoma treated with Bleomycin alone, enlarged lymph nodes clearly schrank following treatment, and a remarkable effect appeared promptly in 5 of them. In the other 2 cases treated with Bleomycin and fibroblast-inhibiting agents, there occurred both tumor regression and improvement of host conditions. The latter agents appeared to prevent. the development of pulmonary complications. In some cases of leukemia treated with Bleomycin, the leukocyte counts were decreased. Hematopoietic function of bone marrow was not disorderd by Bleomycin, but attention shoud be paid to its side effects for the lungs. The clinical experiences with Bleomycin indicate that the drug might well be used as a remission inducer, and expected to improve the chemotherapy in the treatment of malignant lymphoma, but its life prolongation effect in leukemic cases needs further observation. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 Bleomycinの造血器に及ぼす影響に関する研究 第2編 Bleomycinの正常海猽骨髄栓球系造血に対する影響について 67 70 EN Ikuya Kunimasa In order to observe the influence of Bleomycin on the bone marrow thrombopoiesis, clinical tissue culture of bone marrow from normal guinea pigs was conducted. Bleomycin solutions at various concentration were added directly to the culture media and observations were carried out to see effect of Bleomycin on the function of megakaryocytes of bone marrow. The results obtained show that the thrombopoietic functions of bone marrow were not inhibited in the medium containning 0.0005mg/ml, 0.05mg/ml and 5mg/ml of Blomycin. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 85 3-4 1973 Bleomycinの造血器に及ぼす影響に関する研究 第1編 Bleomycinの正常家兎骨髄白血球系造血に対する影響について 59 66 EN Ikuya Kunimasa In order to observe the influence of Bleomycin on the bone marrow leukopoiesis, clinical Tissue culture of bone marrow from normal rabbits was conducted. Bleomycin solution at various concentrations were added directly to the culture media and observations were carried out on the outgrowth, cell density, wandering velosity of pseudoeosinophils, carbon particle phagocytosis. The experimental results demonstrate that the leukopoietic functions of bone marrow were not inhibited in the medium containning 0.0005mg/ml, 0.05mg/ml and 5mg/ml of Bleomycin. No potential conflict of interest relevant to this article was reported.