岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 有機フッ素剤中毒に関する研究 第2編 有機フッ素剤（Nissol）使用農民の健康調査 295 310 EN Kunio Hashida Health survey was done on farmers using an organofluoride (N-methyl-N-(1-naphthyl) monofluoroacetate) and the following results were obtained. 1. Forty-five point seven percent of the farmers had some complaints after spraying the agent, which included fatigue, headache, nausea, anorexia, diarrhea, insomnia or abdominal pain. 2. Blood pictures of the farmers after spraying the agent indicated slight decrease or no change or hemoglobin and erythrocytes with the tendency of a higher color index than normal, and leukocytosis with neutrophilia. These blood changes, however, returned to normal in 6 months after the use of the agent. 3. Increased urinary urobilinogen and glycosuria were present in some cases, but none showed proteinuria. 4. Liver function tests revealed an increased icterus index and positive CCLF in some cases, but no abnormalities were found in TTT, ZTT, transaminases. There appeared to be no clinically appreciable liver disease. 5. ECG showed slight bradycardia and elongated P-Q intervals within normal ranges but no arrhythmia. No heart disease was clinically manifested in any cases. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 有機フッ素剤中毒に関する研究 第1編 有機フッ素剤中毒の治療を主とした実験的ならびに臨床的研究 273 293 EN Kunio Hashida Treatment experiments and blood sugar determinations were carried out on organofluoride-intoxicated animals and the results were as follows. 1. FAM (monofluoroacetamide)-intoxicated rats were treated with various acetyl radical transferring compounds of which acetamide was found to be most effective and resulted in 100% recovery of the animals treated. Treatment with ethanol monoacetin, and glucose led to the prolongation of survival times of the intoxicated rats. Some increases of the survival times and anticonvulsive effects were obtained also by the use of phenobarbital and procainamide. 2. Administration of acetamide and procainamide to FAM-intoxicated rabbits resulted in approximately twice as longer survival times as those of the controls. 3. FAM administered rats showed a general tendency to low blood sugar levels, and the more the dosage of FAM was given, the greater the degree of hypoglycemia was observed. The maximum decrease was noted in the midst of or before and after convulsions of the administered rats. Methylparathion and Endrin administered rats, on the contrary, indicated increased levels of blood sugars. These observations suggest that determination of blood sugar levels can be a clue to differentiating organofluoride-intoxication from organic phosphate and chloride (especially Endrin)-intoxications which occur frequently in Japan. And this point may contribute to the understanding of the etiology of organofluoride-intoxication. 4. A close relationship between unconsciousness and a decrease in blood sugar levels was obtained in a MNFA (N-methyl-N-(1-naphthyl) monofluoroacetate)-intoxicated human case. Administration of large doses of glucose was found to be very effective for the recovery of unconsciousness. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 SV40DNAによるマウス胎児培養細胞の試験管内発癌について 267 271 EN Shinichiro Yamamoto Kuniyuki Egusa Takuzo Oda The oncogenicity of SV 40 DNA was examined in mouse embryo cell cultures in vitro. T antigen induction in mouse embryo cells by SV 40 DNA was predominant at 5 days after infection and T antigen positive cells were observed by more than 10% at this stage. They decreased with increasing time until about 30 to 50 days of culture, after which numbers of cells with positive T antigen increased again to 36 to 38% with morphological changes accompanied. Morphological alterations seen at about 100 days after infection were characteristic of SV 40 transformed cells, e.g., criss-cross appearance, giant cell formation and irregurality of cell arrangements. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 昭和43年度および44年度Acatalasemia, Hypocatalasemia調査集計 259 265 EN No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 ヒトの胃の内分泌細胞の細胞学的研究 第�U編 病的状態の胃前庭部粘膜における内分泌細胞の組織化学的・電子顕微鏡的研究 247 258 EN Akira Nabeyama Normal and pathological antral mucosa (gastric ulcer, gastroduodenal ulcer, duodenal ulcer, gastric cancer, giant rugae, gastritis chronica and gastric polyp) were examined histochemically and electron microscopically. 1) It was clarified that the Ec-cells are observed only in the mucosa with intestinal metaplasia, and never in the normal mucosa. We tentatively classified the Ec-cells into two types; Ec-�T-cell and Ec-�U-cell. The specific granules in the Ec-�T-cells are polymorphous and contain a dense core enclosed by a limiting membrane which fits either tightly or loosely. The Ec-�U-cells have electron dense and polymorphous granules but do not contain a dense core in the limiting membrane. The ratio of Ec-�T-cells to Ec-�U-cells in the antral mucosa with intestinal metaplasia seems to be almost equal in gastric ulcer but smaller in gastric cancer. 2) Cell type �W-cell in the normal antral mucosa has three kinds of granules; electron dense granules (100-200 mμ in diameter), granules (about 400 mμ in diameter) which have cloud-like or bubble-like substances in the limiting membrane, and granules which are empty-looking in the limiting membrane. In gastric ulcer cases, the �W-type cells were apt to be granulated but there were no increse in the number. On the contrary, in atrophic gastritis, the �W-type cells had low electron dense granules which were empty-looking in the limiting membrane. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 ヒトの胃の内分泌細胞の細胞学的研究 第�T編 ヒトの正常胃前庭部粘膜にみられる内分泌細胞の電子顕微鏡的分類 235 245 EN Akira Nabeyama The endocrine cells in te normal antral mucosa of the human stomach were examined by the electron microscope. According to their fine structural characteristics, they were classified into the following five types. 1) Cell-type �U is triangular in shape. The granules are round and vary in diameter (300-600mμ) and in electron density. 2) Cell-type �V is triangular in form and has many grnules (130mμ in diameter) with high electron density. These granules are characterized by their uniform size and homogeneous appearance. 3) Cell-type �W contains a prominent endoplasmic reticulum in the supranuclear region. These kinds of granules are recognizable in the basal part: smaller ones (100-200mμ), which are dense, larger ones (400mμ) which are pale and another larger ones (400mμ) which are empty-looking. 4) Cell-type �Xis contact with the glandular lumen and contains numerous secretory granules with various densities. 5) Cell-type �Y is oval in shape and characterized by small granules (100-150mμ) with moderate density, localized in the basal region. This cell type seems to have some features of an immature endocrine cells. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 83 7-8 1971 SV40誘発腫瘍細胞の長期培養による変化について 229 233 EN Shinichiro Yamamoto Shuji Seki Sachiko Omura Takuzo Oda SV 40 induced hamster tumor cells were cultured in vitro for long terms. Morphological changes and vicissitudes of T antigen and oncogenity were examined in cultured cells. Morphological appearance. has changed from fibroblastic to epitheloid. T antigen and oncogenicity decreased with increasing time, both of which at last disappeared at about 300 days of culture. The significance of the findings is discussed. No potential conflict of interest relevant to this article was reported.