start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1223
end-page=1227
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A Histochemical Study of Cytoplasmic Bodies in the Thymo-lymphatic Tissues of the mouse
kn-title=胸腺リンパ組織内に出現するcytoplasmic bodyの組織化学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=At the beginning of the 20th century, the cytoplasmic bodies which appear in the lymphoid tissues of mammals had received much attention of haematologists, in the relation to the origin of the blood platelets. However, since Wright described and figured in 1910 the process of cytoplasmic budding of megakaryocytes by the use of his own technics, the biological significance of the cytoplasmic bodies in the lymphoid tissues has been ignored until now. Recent works on the formation of antibodies in the lymphoid tissue seem to suggest, on the other hand, that these bodies would play an important role in the immune response. Consequently, we attended a renewed study of these bodies. In this paper we described the results of histochemical observations on these bodies in, the mesenteric lymph node and thymus of adult mice. The chief findings are as follows: 1. Cytoplasmic bodies are found in large number in the mesenteric lymph node of adult mice. They also occur in the thymus, though in much smaller numbers. 2. Cytoplasmic bodies in the thymolymphatic tissues vary greatly in size, from the size of blood platelets to that of small lymphocytes, and are commonly round in shape. They often contain pyroninophilic granules. 3. Staining of these bodies with methyl green-pyronin and acridine orange revealed that they contain abundant RNA. However, no evidence was obtained for the presence of DNA in these bodies. 4. After digestion of the samples with RNase, the pyroninophilia of these bodies disappeared completely. This suggests that the pyroninophilic granules of these bodies might be regarded as ribosomes.
en-copyright=
kn-copyright=

en-aut-name=UekiAyako
en-aut-sei=Ueki
en-aut-mei=Ayako
kn-aut-name=植木絢子
kn-aut-sei=植木
kn-aut-mei=絢子
aut-affil-num=1
ORCID=

en-aut-name=AsanoKazuyuki
en-aut-sei=Asano
en-aut-mei=Kazuyuki
kn-aut-name=浅野一幸
kn-aut-sei=浅野
kn-aut-mei=一幸
aut-affil-num=2
ORCID=

en-aut-name=OhmuraMorito
en-aut-sei=Ohmura
en-aut-mei=Morito
kn-aut-name=大村守人
kn-aut-sei=大村
kn-aut-mei=守人
aut-affil-num=3
ORCID=

en-aut-name=OsogoeBunsuke
en-aut-sei=Osogoe
en-aut-mei=Bunsuke
kn-aut-name=尾曽越文亮
kn-aut-sei=尾曽越
kn-aut-mei=文亮
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1解剖学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部第1解剖学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部第1解剖学教室

affil-num=4
en-affil=
kn-affil=岡山大学医学部第1解剖学教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1211
end-page=1222
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Antimitotic Action of the Cornin, as a Biologically Active Polypeptide (�W)
kn-title=生物学的活性 Polypeptide "CORNIN" の細胞分裂に及ぼす影響（IV）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The difference of the effect of cornins extracted from various kinds of living tissues was examined by employing several cell lines in vitro. And the inhibitory effect on tumor growth of the cornins was tested on the mice transplanted with Ehrlich ascites carcinoma. The results are as follows: 1) The antimitotic effect of canine intestine cornin (CIC) was more effective on Ehrlich ascites carcinoma cells in vitro (JTC-11) than that of bovine intestine cornin (BIC) and that of porcine intestine cornin (PIC). 2) Dialysable fraction, undialysable fraction and crude of CIC showed similar activities on the JTC-11 cell line. 3) The antigenicity of CIC was not proved by the hemagglutination test and no mice injected intraperitoneally with large doses of CIC showed any intoxication and disturbance. 4) The inhibitory effect of CIC on tumor growth was the strongest in the three kinds of the intestine cornins with Ehrlich ascites carcinoma and some of tumor-bearing mice were cured completly by the intraperitoneal injections of CIC. 5) The cornin extracted from liver of normal rats (RLC) had marked inhibitory effect on the cell growth of the short-term cultured cell line (RLM-1) and the long-term cultured cell line (RLN-10) originated from normal rat liver, but no effect was observed on the cell line in culture which came from the DAB ascites hepatoma of the same line rat.
en-copyright=
kn-copyright=

en-aut-name=KimotoKatsuhiko
en-aut-sei=Kimoto
en-aut-mei=Katsuhiko
kn-aut-name=木本克彦
kn-aut-sei=木本
kn-aut-mei=克彦
aut-affil-num=1
ORCID=

en-aut-name=FujitaTakashi
en-aut-sei=Fujita
en-aut-mei=Takashi
kn-aut-name=藤田興
kn-aut-sei=藤田
kn-aut-mei=興
aut-affil-num=2
ORCID=

en-aut-name=KobayashiYoshiji
en-aut-sei=Kobayashi
en-aut-mei=Yoshiji
kn-aut-name=小林芳治
kn-aut-sei=小林
kn-aut-mei=芳治
aut-affil-num=3
ORCID=

en-aut-name=TakahashiSeiichiro
en-aut-sei=Takahashi
en-aut-mei=Seiichiro
kn-aut-name=高橋誠一郎
kn-aut-sei=高橋
kn-aut-mei=誠一郎
aut-affil-num=4
ORCID=

en-aut-name=FujiiYoshinobu
en-aut-sei=Fujii
en-aut-mei=Yoshinobu
kn-aut-name=藤井義信
kn-aut-sei=藤井
kn-aut-mei=義信
aut-affil-num=5
ORCID=

en-aut-name=YamadaToshisuke
en-aut-sei=Yamada
en-aut-mei=Toshisuke
kn-aut-name=山田俊典
kn-aut-sei=山田
kn-aut-mei=俊典
aut-affil-num=6
ORCID=

en-aut-name=ChikataYoshiko
en-aut-sei=Chikata
en-aut-mei=Yoshiko
kn-aut-name=智片芳子
kn-aut-sei=智片
kn-aut-mei=芳子
aut-affil-num=7
ORCID=

en-aut-name=OhtsukiHisashi
en-aut-sei=Ohtsuki
en-aut-mei=Hisashi
kn-aut-name=大月恒
kn-aut-sei=大月
kn-aut-mei=恒
aut-affil-num=8
ORCID=

en-aut-name=MurakamiTetuhide
en-aut-sei=Murakami
en-aut-mei=Tetuhide
kn-aut-name=村上哲英
kn-aut-sei=村上
kn-aut-mei=哲英
aut-affil-num=9
ORCID=

en-aut-name=NisidaIsamu
en-aut-sei=Nisida
en-aut-mei=Isamu
kn-aut-name=西田勇
kn-aut-sei=西田
kn-aut-mei=勇
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=4
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=5
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=6
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=7
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=8
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=9
en-affil=
kn-affil=岡山大学医学部第一生理学教室

affil-num=10
en-affil=
kn-affil=岡山大学医学部第一生理学教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1181
end-page=1187
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Pathway of Synthesis of DNA-thymine in Lymphocytes: An Autoradiographic Study
kn-title=リンパ球におけるDNA-thymineの合成経路：オートラジオグラフィーによる研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It is well established that there are two types of lymphocytes. One of these is rapidly dividing and short-lived. The thymic lymphocytes and germinal-center cells of the lymphatic tissues belong to this type. The other is produced at a slow rate and has a long life span. With respect to the incorporation of (3)H-thymidine into DNA, the former type is　characterized by weak labeling autoradiographically, in contrast to intense labeling in the latter type. Experiments in mice have demonstrated that the lymphocytes of the thymus are capable of synthesizing DNA-thymine endogeneously from deoxycytidine which is circulating in a considerable amount and hence to be weakly labeled with injected (3)H-thymidine. In　contrast, the majority of lymphocytes of the lymph nodes were found to be incapable of synthesizing DNA-thymine endogeneously from deoxycytidine and hence to be intensely labeled with injected (3)H-thymidine. When the pathway of synthesis of DNA-thymine endogeneously from　deoxycytidine was blocked by the administration of 5-fluoro-2'-deoxycytidine, the labeling intensity of the lymphocytes of the thymus with (3)H-thymidine was greatly increased. Experiments in vitro with or without adding cold deoxycytidine to the medium provided further evidence for the utilization of deoxycytidine for the synthesis of DNA-thymine in the lymphocytes of the thymus.
en-copyright=
kn-copyright=

en-aut-name=OsogoeBunsuke
en-aut-sei=Osogoe
en-aut-mei=Bunsuke
kn-aut-name=尾曽越文亮
kn-aut-sei=尾曽越
kn-aut-mei=文亮
aut-affil-num=1
ORCID=

en-aut-name=UekiAyako
en-aut-sei=Ueki
en-aut-mei=Ayako
kn-aut-name=植木絢子
kn-aut-sei=植木
kn-aut-mei=絢子
aut-affil-num=2
ORCID=

en-aut-name=NakaharaHiroko
en-aut-sei=Nakahara
en-aut-mei=Hiroko
kn-aut-name=中原裕子
kn-aut-sei=中原
kn-aut-mei=裕子
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一解剖学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部第一解剖学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部第一解剖学教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1167
end-page=1180
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Tissue Culture of Spleen Chapter �V Vital Observation of Splenic Reticulum Cells by Fluorochrominized Tissue Culture
kn-title=脾組織培養に関する研究 第3編 脾螢光培養法と脾細網細胞を中心とせる螢光観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1) Fluorochrominized tissue culture of spleen was established by the similar method used for bone marrow culture devised in our clinic. And vital observation of splenic reticulum cells was done by fluorochrominized tissue culture method by the use of rectangular cover slip method which has been mentioned in the chapter I. 2) Each type of reticulum cells showed characteristic fluorochrominized findings when stained with acridine orange. Granules and vaculoles stained with acridine orange showed nearly similar findings to neutral red granules and vacuoles as described in the chapter �U. 3) By this method, the existence of transitional forms between each type of reticulum cells and maturation process were confirmed in the series of types �T, �U and �V reticulum cells, as have been already described by other methods in the chapter �T and �U. 4) Part of acridine orange stainable vacuoles were thought to be secondary to toxic effects of acridine orange stain. 5) Changes of fluorochrominized granules and vacuoles were observed successively in the degenaration of type �V reticulum cells.
en-copyright=
kn-copyright=

en-aut-name=KibataMasayoshi
en-aut-sei=Kibata
en-aut-mei=Masayoshi
kn-aut-name=木畑正義
kn-aut-sei=木畑
kn-aut-mei=正義
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1159
end-page=1166
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Tissue Culture of Spleen Chapter �U Vital Observation of Splenic Reticulum Cells by Vital Staining
kn-title=脾組織培養に関する研究 第2編 脾細網細胞の中性赤並びにヤーヌス緑Bによる生体染色及び墨粒貪喰に就いて
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Janus green B and neutral red were used for vital staining of mouse splenic reticulum cells that have been described in the chapter �T and phagocytic activities were observed by carbon particle feeding. 1) With my estabished method, observation of vital staining of splenic reticulum cells were possible under condition avoiding deletrious effects of stain after various time intervals of culture. 2) Each type of reticulum cells which were classified in the chapter �T was characteristically separated by vital staining method. �@) Neutral red granules and vacuoles increased in sine and number. On the other hand, janus green B granules decreased and/or vanished, in the stage of �T, �U and �V type of reticulum cells. These findings showed that type �T reticuum cells matured into type �Uand then type �V reticulum cells in this order. �A) In the cytoplasm of type �V reticulum cells, markedly enlarged red vaculoes and phagocytized stainable materials were observed. 3) Some of the neutral red stainable materials were vaculoes induced by toxic effects of neutral red and janus green B stain. 4) Reticulum cells cultured over 120 hours were stained immediately and faded sooner, in contrast to reticulum cells in the relatively early stage. This phenomenon was thought to be related to the declining function of the cell. 5) By addition of carbon particles to medium, most of type �T and �U reticulum cells showed little phagocytic ability, but type �V reticulum cells manifested active phagocytosis so that their cytoplasm was filled with carbon particles. That was considered to be specific function to type �V reticulum cells.
en-copyright=
kn-copyright=

en-aut-name=KibataMasayoshi
en-aut-sei=Kibata
en-aut-mei=Masayoshi
kn-aut-name=木畑正義
kn-aut-sei=木畑
kn-aut-mei=正義
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1151
end-page=1158
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Tissue Culture of Spleen Chapter �T Vital Observation of Splenic Reticulnm Cells by Phase Contrast Microscopy
kn-title=脾組織培養に関する研究 第1編 脾細網細胞の位相差顕微鏡観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Morphology and movement of splenic reticulum cells of the normal adult mouse were observed in the growth zone of tissue culture by phase contrast microscopy and 16 mm micro-cinematography. 1) For the vital observation of these cells, rectangular cover slip method proved satisfactory. 2) In tissue culture of normal mouse spleen, lymphocytes appeared initially untill 24 hours, follwed by some types of reticulum cells which increased after 24 to 48 hours of culture, and finally fibroblasts increased in the growth zone. 3) According to the morphology, function and degree of maturation, these reticulum cells could be classified into three groups; �T, �Ua, �Ub, �Va and �Vb. There were intermediate types between these cells and type �T reticulum cells matured into type �U and �V reticulum cells. 4) Type �Ua reticulum cells possessed multiple pseudopods and showed active migratory movement, their nucleus locating in the forwarding part of the cytoplasm.
en-copyright=
kn-copyright=

en-aut-name=KibataMasayoshi
en-aut-sei=Kibata
en-aut-mei=Masayoshi
kn-aut-name=木畑正義
kn-aut-sei=木畑
kn-aut-mei=正義
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1145
end-page=1150
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Comparative Observation on the Morphology of Measles and Distemper Viruses
kn-title=麻疹およびジステンパーウイルス粒子の形態的比較観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The particles of measles and distemper viruses have been examined in the electron microscope and the technique of negative staining was used. The diameter of virus particles varies from 120mμ to 600mμ and the structures of the viruses are very similar for each other. The particles have an internal herical component about 18-20mμ diameter, enclosed in the outer membranes with the spikes. The virus particles are pleomorphic.
en-copyright=
kn-copyright=

en-aut-name=TawaraJutaro
en-aut-sei=Tawara
en-aut-mei=Jutaro
kn-aut-name=俵寿太郎
kn-aut-sei=俵
kn-aut-mei=寿太郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部微生物学教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1123
end-page=1143
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A Clinical Study of Borderline Case
kn-title=いわゆる境界例についての臨床的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A clinical name of "Borderline Case" is generally used for such a case which is very difficult to diagnose as schizophrenia or neurosis. It is inevitable that the various cases with different genesis and symptomatology are classified in the borderline case, as the concept and the diagnostic standpoint of schizophrenia or neurosis are not uniform. Although there are many papers on the psychodynamic structure of the borderline case, the clinical pictures of this case are not the same. The author feels that the further clinical observation and the classification are also necessary as well as the psychodynamics of the borderline case. 17 cases are observed and classified into 3 types, who had been the in-patient during two years, from Jan. 1961 to Jan. 1963, at the Dep. of Neuro-Psychiatry, Okayama University Hospital. 1. Pseudoneurotic Type Various neurotic symptoms such as anxiety, phobia, obsession and neurasthenic symptom are flourish. Thought disorder or the superficial emotional contact are prominent in most cases, but autism, the primary schizophrenic disorder is not remarkable in some of these cases, although Hoch, P. insisted that pseudoneurotic schizophrenia is not the borderline case, but a sub-type of schizophrenia. 2. Type of Character Deviation Symptoms are vague and transient, and the disorders of the social life due to the various abnormal behavior and the distortion of the inter-personal relationship are characteristic. Severe disorder of the personality which is difficult to differentiate from psychopath or simple schizophrenia is cardinal in this type. 3. Mono-symptomatic Type Delusional interpretation about his shape, expression or smell persists for a long period. The appearance of the symptom is closely related with the special situation, the presence of other peoples, and the disorder of the personality is minimum. In these cases, the neurotic picture is more evident than in the other types.
en-copyright=
kn-copyright=

en-aut-name=ShohmoriAtsuko
en-aut-sei=Shohmori
en-aut-mei=Atsuko
kn-aut-name=庄盛敦子
kn-aut-sei=庄盛
kn-aut-mei=敦子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部神経精神医学教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1119
end-page=1121
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Class of Speech Correction Established in a Primary School
kn-title=岡山市出石小学校に開設された言語治療教室について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It is a complicating and also urgent problem to educate and rehabilitate the speech handicapped, esp. school-children. And now a trend to teach such handicapped children as a group is increasing in Japan. In May, 1968, two classes for speech correction were established at Izushi Primary School, Okayama City. They consisted of two teachers specially educated and sixteen children with various speech disorders.
en-copyright=
kn-copyright=

en-aut-name=OguraYoshio
en-aut-sei=Ogura
en-aut-mei=Yoshio
kn-aut-name=小倉義郎
kn-aut-sei=小倉
kn-aut-mei=義郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部耳鼻咽喉科学教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1105
end-page=1117
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on (90)YCl(3) Colloid for Clinical Use Part 2. Experimental and Clinical Use by Intraperitoneal and Intralymphatic Administration of (90)YCl(3) Colloid
kn-title=(90)YCl(3)コロイドに関する基礎的研究 第2編 腹腔内およびリンパ管内注入について―動物実験ならびに臨床成績―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=It is an expectation on radiocolloid therapy from the standpoint of prevention of side efects, that the administered radiocolloid is not removing in blood stream or in some organs. Old (90)YCl(3) colloid at pH 7.0 (20mμ diameter) and new type of a mixture of YCl(3) and (90)YCl(3) colloid at pH 7.0 (3-15μ diameter) were administered into peritoneal cavity of mice and rabbits. The distributions of administered (90)Y in serum and organs were compared. Also, these two colloids were examined by intralymphatic administration for clinical use, and the distributions were observed in serum and the excretion of urine. 1) (90)Y in peripheral blood by peritoneal administration of mice were higher in old(90)YCl(3) colloid than that of new type of YCl(3) and (90)YCl(3) mixture. The c. p. m. in peripheral blood were generally decreased by addition of YCl(3). Minimum distribution in peripheral blood was obtained by addition of 1mg/ml of YCl(3). 2) The same results were obtained from rabbit experiment. Old (90)YCl(3) colloid was 2 times of c. p. m. than that of new type mixture colloid at 7th day. (90)Y excretion in urine of old (90)Y colloid was 51 times than that of new type colloid after 3 hrs colloid administration. The distributions of old (90)YCl(3) colloid were spleen, kidney, liver, marrow, lung, heart muscle, and thigh, in the orders, 7 days after administration, and that of new type colloid were spleen, liver, marrow, lung, kidney, heart muscle, and thigh. (90)Yc. p. m. of old colloid was 4 times than that of new colloid in kidney, heart muscle and thigh. New colloid was 5 times than old colloid in spleen, liver, marrow and lung. 3) (90)Yc. p. m. in peripheral blood of intralymphatic administration was observed in both colloid, below 1% excretion in urine of administered dose after 3 days. In clinical use, one case was visible reduction of metastatic lymphnode, but clinical effects were not clear, for control patients were end stadium of malignant tumor.
en-copyright=
kn-copyright=

en-aut-name=TachibanaAkihisa
en-aut-sei=Tachibana
en-aut-mei=Akihisa
kn-aut-name=立花明久
kn-aut-sei=立花
kn-aut-mei=明久
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1091
end-page=1103
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on (90)YCl(3) Colloid for Clinical Use Part I. Purification of (90)YCl(3) Colloid by Milking Method and the Observation of Electrone Microscopy
kn-title=(90)YCl(3)コロイドに関する基礎的研究 第1編 1)イオン交換樹脂法(milking system)による(90)YCl(3)分離採取時の混入(90)Srの検出ならびに除去に関する研究 2)各種(90)YCl(3)コロイドの電顕的観察について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=(90)Y is a high-energy radioisotope and emite β-ray with 2.6 days half life. (90)YCl(3) colloid has been used for malignant tumor therapeutically, as (198)Au colloid. In the present experiment, the purification of (90)Y, and the detection of the contaminated (90)Sr was studied. Amberlite XE-100 column was suitable for this prupose. The colloidal size of (90)YCl(3) was transformed by pH, and the features of (90)YCl(3) colloids were observed on electrone microscope. 1) (90)Y and (90)Sr mixture adsorbed on Amberlite XE-100 column (100-200 mesh, 2.5 column volume), and eluted by 0.5 N HCl. (90)Sr is eluted out at 10-60 ml, and (90)Y is eluted out from 170 ml. 2) A mixture of 20 mC of citrate-(90)Y complex and 1μC of (90)Sr fixed on this column, and then eluted by 0.5 N HCl. One drop of eluate at 10 ml was detectable by GM counter. The detectable lower limit of contaminated (90)Sr is 0.1μC by re-counting on GM counter 24 hrs after the elution. 3) Amberlite XE-100 column is suitable for the second column on the milking system, for the purpose of separating the contaminated (90)Sr and (90)Y. This procedure is complete in 1 hr process. 4) (90)YCl(3) colloid is aggregated and formed clamp in high pH solution, Electrone microscopic observations showed that the colloidal size was 40-50 mμ at pH 5, and 20 mμ at pH7.
en-copyright=
kn-copyright=

en-aut-name=TachibanaAkihisa
en-aut-sei=Tachibana
en-aut-mei=Akihisa
kn-aut-name=立花明久
kn-aut-sei=立花
kn-aut-mei=明久
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1081
end-page=1090
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Ultrasonic Diagnosis in Surgery Part 2. Ultrasonic Diagnosis of Liver Disease Combinating with Colloidal Au(193) Hepatoscintigram & Using the Ultrasonic Contact Compound Scope
kn-title=外科領域における超音波診断に関する研究 第2編 超音波による肝腫瘍診断に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The high request of the preoperative diagnosis for liver metastasis of cancer is the claimed factor in the surgical field. Many kinds of examinations; i. e, physical examination, liver function test of serum, liver biopsy, laparoscopy, roentogenological diagnosis and others were using for this purpose, but the diagnosis was unliable yet. Recently, the clinical application of radioisotope is remarkably advanced, and the scintiscanning is used as a standard tool for clinician. The advances of the ultrasonic diagnostic apparatus and techniques is also evident. In this paper the investigations were carried out by combinating method of ultrasonic diagnosis with hepatoscintigram. The excellent point of this method is to improve the weak points each other of the hepatoscintigram and ultrasonic diagnosis. By means of hepatoscintigram every large space occupying lesion (more than 2-3cm in diameter) in liver can be seen but the depth and structure of the lesion can not be recognized, however the ultrasonic A-scope method is able to clarify the laters. Ultrasonic method is able to diagnose the character of the aimed occupying lesion, respectively. Generally, the continuous multiplying echo indicate the cancer tissue, the boundary wall echo is the cyst or abscess, and the gall stone is easily diagnosed at the same time. But the risk for over diagnosis is respective in two methods, the diagnosis by this combinating method must be carefull in considerations. Combinating method of ultrasonic A-scope with hepatoscintigram gave a good result for liver disease. But the nature of the echoes obtained various kind of livers tend to over-lap. Therefore it is difficult to remark the distinguished accuracy in all cases. The suitable frequency was 2.25MC and a barium titanate of 10mm in diameter was used. Now we are using the ultrasonic contact compound scope, Aloka SSD-10. In this paper some results and problems of this method were presented. The examination is carried out at the lower right chest wall or through the abdominal wall. Several normal human livers were examined first. Some echograms of them were shown. Details of the deep structure are shown when STC (Sensitivity Time Control) is on. But concerning intrahepatic structure, it demonstrates more clearly than when STC off. Some echo patterns of the metastatic liver cancers were shown. A patient who had alarge tumor at his upper right abdomen was examined. His echogram of liver is shown. There is a large tumor (4cm in diameter) at the deep region in liver and the back surface of liver is pressured backwards. But no malignant echo pattern is seen. In some cases of our examinations malignant tissues showed only the surface and bottom echoes. The result of operation actually showed the metastatic cancer of liver, corresponding with the echogram. This fact suggests there are various degrees of absorption of ultrasound among cancers.
en-copyright=
kn-copyright=

en-aut-name=ChikubaHiroshi
en-aut-sei=Chikuba
en-aut-mei=Hiroshi
kn-aut-name=竹馬浩
kn-aut-sei=竹馬
kn-aut-mei=浩
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=80
cd-vols=
no-issue=11-12
article-no=
start-page=1071
end-page=1080
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1968
dt-pub=19681230
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on the Ultrasonic Diagnosis in Surgery Part 1. Ultrasonic Examination of Intracerebral Destructive Lesion in Stereotaxic Surgery
kn-title=外科領域における超音波診断に関する研究 第1編 定位脳手術破壊巣の超音波診断に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Stereotaxic thalamic lesions by electrocoagulation have been produced on sixty four cases of extrapyramidal disorders by means of newly deviced simple electrocoagulation probe, which is made of 300mm of straight steel wire with one millimeter in diameter covered loosely by 293mm of hard teflon pipe as insulation to electricity and the local heat. The probe, therefore, has retractable 7mm bare tip, so it can be safely withdrawn without any, danger from hemorrhage of destructed tissue after coagulation. As a preliminary experiment, stereotaxic thalamic lesions by the probe have been produced in the dog brain as well as the formalin suspended human brains under observation with ultrasonic A-scope apparatus by reflection method. The transducer provided with barium titanate (2.25 Mc, 10mm diameter) was used. It was clearly identified that the echoes were corresponded to size and location of the lesions in the dog brain as well as human brain specimen. During the surgery, echo was noted from the tip of probe which was in the target area of the thalamus of the patients. In every case, the lesion echoes showed high fluctuating amplitude and width during coagulation procedure. Lesion echoes were observed up to 45 days after the surgery. They maintained stable duplicate echoes during the period. It is our suggestion that the ultrasonic examination of the stereotaxic lesion is useful to note accidental hemorrhage in the lesion of a reconvalescent patient. It is also a great aid for this purpose to check the midline echo at the same time. Recently further investigations with ultrasonic compound sector scope, Aloka SSD-10, were done. The ultrasonic scanning was performed on the operative side vertically. During and after the coagulation of the target, twenty three cases were examined with compound sector scope. The reflextion from the lesion with compound scope was noted in 91.3% of the cases while 82.9% with A-scope. The lesionecho was obtained about 1.4cm lateral from the midline echo in case of destruction of V.L. nucleous of the thalamus. In case of the centre median the lesionecho was located adjacent to the midline echo. These lesionechoes were located correctly in their anatomical coordinates of the targets. But the size and shape of the lesionechoes were identified rather larger than the destructed lesions. This deflexion would be due to the width of the ultrasonic reflected wave. As confirmative experiments, stereotaxic thalamic destructive lesions have been produced in the specimen of the formalin suspended human brains under observation with ultrasonic compound sector scope. It is our impression that echo-encephalgraphy with the ultrasonic compound scope has a potential usefulness to observe location, shape and size of the lesion as well as to detect the accidental hemorrhage of a reconvalescent patient.
en-copyright=
kn-copyright=

en-aut-name=ChikubaHiroshi
en-aut-sei=Chikuba
en-aut-mei=Hiroshi
kn-aut-name=竹馬浩
kn-aut-sei=竹馬
kn-aut-mei=浩
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科教室
END