岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 隠岐諸島小中学校学童の医学的調査について 721 726 EN Yoshio Ogura Yozo Orita Noboru Asahi Kazuo Chiba Yutaka Kondo Hiroshi Sugata A series of medical examinations was performed on school-children of Oki Is., Shimane Pref. in April, 1967. The investigations consisted of screening tests for acatalasemic gene carriers, ABO blood type tests, and ENT examinations. Results obtained are as follows: 1. ENT examinations and blood type tests showed average grade and value as those of other places of Japan. 2. Screening tests: Seven hypocatalasemic cases were found out among 4,831 children examined. Then, emergence of hypocatalasemia on Oki Is. was 0.15% , near to already known Japanese mean value. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 Forel-H野破壊術の基礎的ならびに臨床的研究 第2編 定位的Forel-H野破壊術の臨床的研究 703 719 EN Toshio Hiroe Ninety-six operations of Forel-H-tomy were performed on fifty epileptic patients between 1960 and 1964. Follow-up observation of these fifty cases could reach to three years and eight months at the longest and a month at the shortest cases. In eight cases of those patients electrodes were inserted stereotaxically in the Forel-H field to make some physiological study. 1. In nineteen cases (38 per cent), a complete alleviation of grand mal, in twenty-two eases (44 per cent), a moderate alleviation of grand mal were observed within postoperative three months. But there are nine cases (18 per cent) of no effect. Postoperative follow-up could reach to six moths or more in forty-two cases. In fourteen cases (33.3 per cent), marked effect, in twentyone cases, moderate, and seven cases, no effect, were observed. The operative long-term effect more or less decreases when the follow-up is extended over a longer period. 2. The side effect of this procedure was mostly transient and demonstrated light contralateral hemiparesis. In fourteen cases this was observed, but remaine in only five cases for longer period. 3. The pattern of EEG recorded from the Forel-H field was different from that of the scalp leads. The electric stimulation of the Forel-H field could not inflict on the scalp EEG. 4. EEG follow-up has been carried out in all cases but no detectable change was observed between the pre- and the post-operative EEG. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 Forel-H野破壊術の基礎的ならびに臨床的研究 第1編 Strychnine NeuronographyによるForel-H野の研究 687 701 EN Toshio Hiroe Thirty-five adult cats were utilized for this study. The animal was artificially ventilated after administration of succinil cholin chloride in sufficient doses to abolish respiratry and somatic movement. Bipolar concentric electrodes, each having an outside diameter of 0.8mm, were inserted stereotaxically into selected cortical and subcortical structures. As a stimulation of the cerebral cortex and subcortical structures, 0.01-0.05ml of 0.1% strychnine was applied. 1) Forel-H field was not effectively stimulated by administration of strychnine. 2) The stimulation of the cortex (gyrus sigmoideus anterior) elicited frequently spike discharges in the Forel-H field. 3) The stimulation of the thalamus (N. ventralis lateralis) was not followed by so frequent clear spike discharges of Forel-H feild as the stimulation of the cortex. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 脳溢血の外科的療法の適応に関する実験的研究 第2編 脳波学的研究 679 685 EN Osamu Mohri Intracerebral hematoma was experimentally produced by injection of arterial auto-blood into the area of the internal capsule with stereotaximetry in dogs. Forty-eight hours after injection of blood the hematoma was aspirated, the aspirated and the non-aspirated cases were followed electroencephalographically. EEG recording showed localized or generalized slow wave activity immediately after the intracerebral blood injection, which continued even after two months in non-aspirated cases. Marked decrease of the slow activity was observed shortly after aspiration and EEG returned to almost normal pattern after one week. According to the results, it was made clear that the exstirpation of blood clot was effective of the prevention of functional disturbance of nerve cells caused by intracerebral hematoma. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 胃癌の水解および脱水素酵素の組織化学的研究 第2編 胃癌の臨床像と癌組織の酵素活性との関連性について 655 662 EN Kisaburo Takeuchi Histochemical analysis of enzyme activities of thirteen enzymes in operated stomachs of 100 patients were compared with their clinical findings. Materials and methods of histochemical analysis were the same as the previous report. Enzyme activities in the stomach cancer cells of the patients, who showed serum hypoproteinemia, decrease of blood hemoglobin content, and increase of erthrocyte sedimentation rate, were stronger than those in the patients who had normal level of the above tests. While the relationship between enzyme activities in the stromal elements and the values of clinical tests in those groups showed the reverse tendency. No parallel relationships were observed between the alkaline phosphatase activity in cancer cells and the serum alkaline phosphatase level. Also no correlation between alkaline phosphatase activity in tumor cells and the patients liver dysfunction or liver metastasis. Leucine aminopeptidase was positive in the cancer cells of 35 per cent cases. Generally, the stomach cancers removed from anacidity or hypoacidity patients had a stronger activity of this enzyme than that of normoacidity patients. In general, enzyme activities were decreased in both of the stomach cancer cells and stromal elements obtained from the preoperatively treated with antitumor agents, such as Mitomycin C, Chromomycin A(3), and Cyclohosphamide than those from non-treated oncs. The patients who showed stronger activities in stromal elements showed distinctly a better prognosis than those weaker activities. While the patients who had stronger activities in cancer cells had worse prognosis than those weaker activities. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 胃癌の水解および脱水素酵素の組織化学的研究 第1編 胃癌組織分類委員会の胃癌の組織学的分類および,胃癌研究会の胃癌取扱い規約と酵素活性との関連性について 637 654 EN Kisaburo Takeuchi Histochemical studies of five hydrolytic enzymes, i. e. alkaline phosphatase, acid phosphatase, beta esterase, beta-glucuronidase, and leucine aminopeptidase and eight dehydrogenases, i. e. lactic, succinic, malic, alpha-glycerophosphate, glutamic, beta-hydroxybutyric, glucose-6-phosphate, and isocitric dehydrogenase were carried out in the gastric cancers removed from 100 patients. Enzyme activities were compared with the histological features of stomach cancers in each patient and the grade of its advancement. Histological analysis of stomach cancer was followed by the classification established in 1962 by the Japanese Pathological Society. And the grade of advancement was judged by the criteria eatablished by the Japanese Reserch Society for Gastric Cancer. As a whole, the poorer differentiated cancer cells showed the weaker enzyme activites than the well differentiated ones. However, no correlations were observed between enzyme activities and the grade of infiltration by cancer cells. Generally enzyme activities of carcinoma simplex were weaker than those of adenocarcinom and scirrhous carcinoma showed slightlly weaker activity than carcinoma simplex. According to the classification of Borrmann, enzyme activites of infiltrative types were weaker than those in localized types. Lactic dehydrogenase showed the strongest staining intensity in all of the enzymes. The activity was observed in hydrolytic enzymes of carcinoma cells in the following desending order: beta-glucuronidase, beta-esterase, acid phosphatase, leucine aminopeptidase and alkaline phosphatase, while in that of stromal elements : beta-glucuronidase, acid phospbaase, beta-esterase, alkaline phosphatase and leucine aminopeptidase. The activity of dehydrogenases of carcinoma cells was in the following desending order: lactic, malic, glucose-6-phoshate, isocitric, succinic, alpha-glycerophosphate, glutamic and beta-hydroxybutyric dehydrogenase, and in that of stromal elements : lactic, malic, succinic, alpha-glycerophosphate which was similar to glutamic, beta-hydroxybutyric similar to glucose-6-phosphate and isocitric dehydrogenase. There was a tendency that the cancer tissues obtained from the patients of more advanced stage showed the stronger activity of enzymes than that of early stage ones. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 精神病者の間脳・下垂体・副腎皮質系機能及び前頭葉関与に関する研究 619 635 EN Kazunori Izumiya As a link in the series of studies on the functions of the diencephalo-pituituitary adrenocortical system in endogenous psychosis, an investigation was carried out on 26 cases of schizophrenia who received the standard bilateral lobotomy, for the purpose to find out how the frontal lobe affects to the endocrine functions and autnomic nervous systems. For this purpose ACTH (25 units) was administered intramuscularly to 17 cases of them, and the m echolyltest was conducted with the other 9 cases, measuring the lelvels of corticoids in serum and urine of both groups. For the controls, ACTH was administered to 9 cases of schizophrenia who did not receive lobotomy. The results of the study may briefly be summarized as follows. 1) In the control group after ACTH adiministration the serum 17-OHCS showed a tendency of a decrease in the cortical reserve capacity, and in the lobotomized group there could be seen an abnormal reaction to ACTH in addition to a decrease in the cortical reserve capacity. 2) Total blood cholesterol after the ACTH stimulation decreased temporarily, showing similar changes up to 30 minutes in both groups, but in the lobotomized group an increase in cholesterol could be observed thereafter, revealing a contrasting phenomenon to the decreasing tendency of the serum 17-OHCS. 3) The blood potassium decreased transiently 10 minutes after the ACTH administration in both groups and such changes were unstable in the control group while the blood potassium in the lobotomized group increased steadily. As for the fluctuations of urine potassium its excretion was decreased transiently after the ACTH administration in both groups, which later rebounded, and this recovery tendency was more marked in the lobotomized group. 4) 17-KS excreted in urine on ACTH stimulation increased accompanying a marked increase in the amount of urine in the case of lobotomized group. 5) The results of the mecholyl test revealed that even the lobotomized patients respond to mecholyl, but there can be observed a decline and delay in the reactivity. 6) Observations on the fluctuations of serum 17-OHCS, urine 17-OHCS and 17-KS conducted simultaneouly with the mecholyl test revealed a declinining tendency in serum free 17-OHCS while total 17-OHCS in urine to be rather increased in the lobotomized group. The quantity of 17-KS excreted in urine decreased at first and then increased, and such a tendency did not parallel with the change in the total 17-OHCS excreted in urine. In the patients showing identically the same pathological conditions there could be recognized no distinct correlation between the type of response to mecholyl and the corticoid picture. 7) While we can recognize a certain correlation between defect symptoms of the frontal lobe and the endocrine functions, when we investigate individual caes, we find complex features in the endocrine function and autonomic nervous system according to the constitution before the lobotomy and the postoperative pathological picture. Namely, in those patients in a quiet mentbal state, though they are not completely cured, both functions maintain the balance in the state of lowered tension. On the other hand, in those who show a premonition of unrest and excitation, both functions have become unstable. Moreover, those who may have hallucination and delusion and are in the state of chronic defect, so long as there occurs no excitation, they show the levels of function close to the normal. 8) Summarizing these findings, it may be said that there is a decreasing tendency of the response to the ACTH stimulation in the functions of the diencephalo-pituitary-adrenocortical system of chronic shizophrenic group, and distribution of the estimated values is larger than that of normal persons, and this tendency is still more pronounced in the lobotomized group. Furthermore, the dissection of the frontal lobe brings about a defective equilibrium in the reaction mechanism of regulating center in endocrine system, but once placed under a strong stimulus, this balance is broken and there occurs a marked disruption in the endocrine rhythm. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 日本脳炎ウイルスの電子顕微鏡的研究 第1編 日本脳炎ウイルス粒子の形態 589 597 EN Masahito Moguruma An electron microscopic study was performed on the ultrastructure of the Mukai strain of Japanese encephalitis virus propagated in vitro on PS cells. PS cells were cultivated in bottles containing 10% calf serum and 0.5% lactalbumin hydrolysate in Earle's balanced solution. Titration of cell-associated virus was estimated by cytopathic effect. At various intervals following infection, the cells were fixed in buffered 1% osminum tetroxide-solution, embedded in methacrylates and cut on a Leitz ultramicrotome. After the sections were stained in saturated uranyl acetate solution, they were observed in the Hitachi type HUll electron microscope. It is suggested that the JE virus particle is hexogonal in shape and approximately 40 mu in the longest diameter, it is composed of an outer membrane 20 A° thick viroplasm 30 A° thick, and an electron dense nucleoid 25 mu in diameter. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 担癌ラッテにおけるステロイドの代謝に関する研究 第2編 in vitroでのステロイドの代謝 585 588 EN Saburo Yamamoto The studies were performed on 3H-cholesterol metabolism in vitro with rat adrenal glands from normal, bearing Walker carcinoma and treated with ACTH. The amount of 3H-cholesterol metabolites containing pregnenolone, progesterone, 11-deoxycorticosterone and corticosterone were determined and compared among these rat adrenals. The cholesterol metabolism in tumor bearing rat adrenal was similar to that of treated with ACTH. These facts suggested that tumor bearing rat adrenals were simulated with ACTH-like substance. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 担癌ラッテにおけるステロイドの代謝に関する研究 第1編 in vivoでのステロイドの代謝 577 583 EN Saburo Yamamoto The cholesterol metabolism in male Wistar strain rat was studied on the biosynthesis of steroid hormone. 3H-cholesteryl ester was completely formed by 5 hours after the 3H-cholesterol administration from tail vein, and were recoginzed the metabolites from 3H-cholesterol in the adrenals, liver and serum after 2 hours. The steroid hormones from 3H-cholesterol observed maximum conversion at 5 hours. The metabolic processes were studied in rat bearing Walker carcinoma. The cholesterol conversion to steroid hormone in vivo in tumor bearing rat was elevated twice than the normal. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 79 7-8 1967 制癌剤の副作用防止に関する研究 551 575 EN Masahiko Murata Preventive measure of the side-effects of an anticancer agent, Mitomycin-C (MMC), was investigated on Ehrlich tumor mice, by using several agents which had originally no tumoricidal effects except last one: such as orotic acid (OA), chloroquine orotate (QO), magnesium chloride (Mg), Sarvinal (SV), dihydrostreptomycin (SM), and so-called OX-substance (OX; unsaturated fatty acid fraction extracted from the liver of X-Ray irradiated rabbit). Results obtained were as follows. 1. Mg had a most preventive effect on the reduction of WBC due to MMC, following by SM, CO and SV. 2. On the fluctuation of RBC, SM, Mg, OA and CO showed better results. 3. No agents tested were effective on the prevention of reduction in platelets. 4. No unwilling decrease in tumor-effects were noted in these agents. 5. Survival time was markedly extended in case of using SM. Summarizing the results obtained above, streptomycin was a most useful agent for the prevention of side-effects of Mitomycin-C. Under the fundamental investigation, streptomycin was clinically used in case of giving Mitomycin-C, and the side-effects were remarkably reduced as seen in the experiment. Further studies would have to be continued on the other anticancer agents. No potential conflict of interest relevant to this article was reported.