| ID | 13914 |
| Eprint ID | 13914
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| FullText URL | |
| Title Alternative | Flowcytometric studies of effects of heat on DNA synthesis in different thermosensitivities mammalian cell lines
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| Author |
Tanaka, Seiryou
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| Abstract | The effect of heat on DNA synthesis in HeLa S3 cells and L-5 cells was studied using flowcytometry. When D(0) values obtained from survival curves of Ehrlich ascites tumor cells, L-5 cells, HeLa S3 cells and NIH3T3 cells after heating to 43, 44, or 45℃, were compared, HeLa S3 cells were resistant and L-5 cells were sensitive to heating. During heating at 43℃, DNA synthesis (BUdR uptake) of HeLa S3 cells was resistant compared to that of L-5 cells. When the period of DNA synthesis was divided into 3 fractions (early S, mid S and late S), late S phase was the most sensitive fraction to heating at 43℃ for 60 minutes. From these results, relationship between DNA synthesis and thermocytotoxic effects are discussed.
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| Keywords | Flowcytometry
DNA synthesis
Heating
BUdR
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| Note | 原著
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| Published Date | 1993-08
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| Publication Title |
岡山医学会雑誌
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| Publication Title Alternative | Journal of Okayama Medical Association
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| Volume | volume105
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| Issue | issue7-8
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| Publisher | 岡山医学会
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| Publisher Alternative | Okayama Medical Association
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| Start Page | 771
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| End Page | 778
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| ISSN | 0030-1558
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| NCID | AN00032489
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| Content Type |
Journal Article
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| Official Url | https://www.jstage.jst.go.jp/article/joma1947/105/7-8/105_7-8_771/_article/-char/ja/
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| Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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| language |
Japanese
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| Copyright Holders | 岡山医学会
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| File Version | publisher
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| Refereed |
True
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| Eprints Journal Name | joma
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