start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=673
end-page=685
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A study of anatomical shape and size of the atlanto-axial joints
kn-title=環軸関節の形態について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The atlas and axis from 52 cadavers, 33 male and 19 female were measured from the outer contour and microradiographs of sectioned samples. The vertical diameter of the anterior arch of the atlas was 11.3mm in men and 10.9mm in women. The height of the body of the axis was 38.1mm in men and 33.8mm in women. The angle between the axes of the dens and the lower surface of the body of the axis was 58.6 degrees in men and 57.1 degrees in women. An anterior buttress plate to prevent atlanto-axial subluxation was designed based on the measurements of this study.
en-copyright=
kn-copyright=

en-aut-name=BitohKaoru
en-aut-sei=Bitoh
en-aut-mei=Kaoru
kn-aut-name=美藤馨
kn-aut-sei=美藤
kn-aut-mei=馨
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部整形外科学教室
en-keyword=環軸関節
kn-keyword=環軸関節
en-keyword=計測
kn-keyword=計測
en-keyword=プレート固定
kn-keyword=プレート固定
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=659
end-page=672
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Expression and purification of the HIV-1 env gene products in Escherichia coli
kn-title=HIV-1エンベロープ蛋白質の大腸菌での発現と精製
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=To purify the HIV-1 envelope protein with antigenic reactivity, the Pvu II-Bgl II fragment of the HIV-1 env gene, from the Pvu II site to the second Bgl II site, encoding the carboxyl terminal 180 amino acids of the viral surface protein (SU, gp120) was molecularly cloned in Escherichia coli strain HB101 using protein A expression-shuttle vector pRIT5. The pRIT5 contains the protein A gene, encoding the secretion signal and IgG binding domain of protein A with the upstream promoter and the downstream multicloning sites, as well as the two replication sites for Escherichia coli and Staphylococcus aureus. A fused protein with the molecular weight of about 55 kilodaltons was produced, which showed the same reactivity as the native protein A against rabbit serum IgG on Western blotting analysis. Most of the fused protein in the periplasmic space was degraded, while the complete fused protein inside the cells was recovered as an insoluble protein. The fused protein was solubilized with sodium dodecylsulfate (SDS), partially purified by IgG sepharose affinity chromatography, and completely purified by SDS-polyacrylamide gel electrophoresis. The quantity of the expressed fused protein was estimated about 1% of the total proteins. The purified fused protein contained 516 amino acids with Mr54, 976, consisting of 305 amino acids of the IgG binding domain of protein A, 5 amino acids derived from polylinker, a carboxyl terminal 180 amino acids of the HIV-1 envelope surface protein gp120, and 26 amino acids derived from the pUC19 sequence.
en-copyright=
kn-copyright=

en-aut-name=ZhangBo
en-aut-sei=Zhang
en-aut-mei=Bo
kn-aut-name=張波
kn-aut-sei=張
kn-aut-mei=波
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌源研究施設生化学部門
en-keyword=HIV-1
kn-keyword=HIV-1
en-keyword=エンベロープ蛋白質
kn-keyword=エンベロープ蛋白質
en-keyword=発現ベクター
kn-keyword=発現ベクター
en-keyword=Protein A
kn-keyword=Protein A
en-keyword=IgG-Sepharoseカラム
kn-keyword=IgG-Sepharoseカラム
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=647
end-page=657
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Analysis of mortality of mental patients
kn-title=精神障害者の死亡と死因に関わる社会医学的要因
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Most previous studies of mortality among mental patients dealt only with an in-patient population or with in-patient and out-patient populations of a mental hospital. A communitybased study was performed to analyze the mortality among mental patients in Kochi Prefecture. Schizophrenics and alcoholics were selected out of all mental patients for analysis. The relative risk of death among male and female schizophrenics and male alcoholics were two or three times higher than that among the general population. Especially, a younger group (30-49 years old) had higher relative risk than an older group (50-79 years old) among both patients. Therefore, to decrease the mortality among the mental patients, it is important to provide adequate health and medical care to younger patients. Among male and female schizophrenics, high relative risks were found for suicide, heart disease and accidents. The relative risk of death among the female patients was higher than that among the male patients. Among male alcoholics, high relative risks of death were observed for liver cirrhosis, suicide, heart disease, accidents, pneumonia and cerebral vascular diseases. In conclusion, the development of a community mental health care system, as well as rapid progress in psychiatric pharmaco-therapy, is necessary to decrease the mortality among mental patients.
en-copyright=
kn-copyright=

en-aut-name=IeyasuHidetaka
en-aut-sei=Ieyasu
en-aut-mei=Hidetaka
kn-aut-name=家保英隆
kn-aut-sei=家保
kn-aut-mei=英隆
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部衛生学教室
en-keyword=精神分裂病
kn-keyword=精神分裂病
en-keyword=アルコール中毒
kn-keyword=アルコール中毒
en-keyword=相対死亡比
kn-keyword=相対死亡比
en-keyword=死因
kn-keyword=死因
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=629
end-page=646
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A study of the effect of ethanol on mercury excretion of mice after the short term exposure to low concentration mercuric vapor - Experiment of the oxidation or reduction of mercury in vitro and vivo - part 3
kn-title=水銀蒸気暴露後の生体内分布とエタノールの影響（水銀の酸化及び還元に関する試験管内及び動物実験 第3編）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Ethanol, 0.8 g/kg or 1.6 g/kg was administered to mice and exposed to mercury vapor ((203)Hg(0)) with the control mice which did not receive ethanol at the concentration of 0.1-0.5mg/m3 for 10 minutes. The exhaled mercury, urinary mercury, mercury in the blood and organ distribution of mercury after 1 hour of exposure of mice administered ethanol compared with those of control mice. The results obtained were as follows. 1. The amounts of exhaled mercury in the mice treated with ethanol were more than that in control mice; and the exhalations of human who receive ethanol will overestimate the true concentration of exposure in the Biological Monitoring. 2. The mercury concentrations in the blood of mice treated with ethanol were less than the control mice. The bloods of human who receive ethanol underestimate the true concentration of exposure in the Biological Monitoring. 3. The concentrations of mercury of urine corrected at a urinary density of 1.024 in mice treated with ethanol at 0.8 g/kg is more, and at 1.6 g/kg is less than the control. The urines of human who receive ethanol in low quantity will overestimate and in high quantity will underestimate the true concentration in the Boilogical Monitoring.
4. The concentrations of mercury of urine corrected at a urinary density of 1.024 have a significant regression to the mercury concentration in the kidney and not to the blood in all group (ethanol and control).
en-copyright=
kn-copyright=

en-aut-name=KurahashiKouzirou
en-aut-sei=Kurahashi
en-aut-mei=Kouzirou
kn-aut-name=倉橋康二郎
kn-aut-sei=倉橋
kn-aut-mei=康二郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=ethanol
kn-keyword=ethanol
en-keyword=mercuric vapor
kn-keyword=mercuric vapor
en-keyword=mice
kn-keyword=mice
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=613
end-page=627
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A study of the effect of DL-α-tocopherol on mercury exhalation and organ distribution in mice injected with mercuric ion - Experiments of the oxidation or reduction of mercury in vitro and vivo - part 2
kn-title=水銀イオン投与後の生体内運命に関する(DL)-α-トコフェロール及びアミノトリアゾールの影響（水銀の酸化及び還元に関する試験管内及び動物実験 第2編）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Mice were preliminarily injected with Vitamin E subcutaneously and with mercuric ion intraperitoneally. The exhalation and organ distribution of mercury were determined and compared with the control mice without Vitamin E. Mice were preliminarily injected with 3-amino-l, 2, 4-triazole intraperitoneally and with mercuric ion intraperitoneally. The exhalation, urinary excretion and organ distribution of mercury were compared with the control mice. The results obtained were as follows. 1. The amounts of mercury in the exhalation of mice which were administered Vitamin E were more than the control. 2. The levels of mercury concentration and perecentages of distribution in the kidneys of mice which received Vitamin E were significantly higher than the control mice. The levels of mercury concentration and percentages of distribution in the carcass and the subcutaneous tissue of mice which received with Vitamin E were significantly less than the control mice, respectively. It suggested that the Vitamin E exerts an effect as the blocker, with accumulation of mercury in the adipose tissue and, eventually, a larger accumulation of kidney.
3. The amounts of exhaled mercury and that in urine in mice which received 3-amino-1, 2, 4-triazole were more than those in the control mice.
This suggested that the reoxidation of mercury (Hg(0)→Hg(++)) in the mice treated with 3-amino-1, 2, 4-triazole was less than in control mice, and that higher amounts of Hg0 resulted, which could be exhaled from the lungs and accumulated into the kidneys.
en-copyright=
kn-copyright=

en-aut-name=KurahashiKouzirou
en-aut-sei=Kurahashi
en-aut-mei=Kouzirou
kn-aut-name=倉橋康二郎
kn-aut-sei=倉橋
kn-aut-mei=康二郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=(DL)-α-tocopherol
kn-keyword=(DL)-α-tocopherol
en-keyword=3-amino-1, 2, 4-triazole
kn-keyword=3-amino-1, 2, 4-triazole
en-keyword=mercuric ion
kn-keyword=mercuric ion
en-keyword=mice
kn-keyword=mice
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=603
end-page=612
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Study of the reduction of mercuric ion by (L)-ascorbic acid, (DL)-α-tocopherol, superoxide anion and the supernatant of mouse liver homogenate - Experiment of the oxidation or reduction of mercury in vitro and in vivo - part 1
kn-title=(L)-アスコルビン酸，(DL)-α-トコフェロール，スーパーオキサイドアニオン及びマウス肝上清分画による水銀イオンの還元（水銀の酸化及び還元に関する試験管内及び動物実験 第1編）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The reduction of (203)Hg(++) by (L)-ascorbic acid, (DL)-α-tocopherol, superoxide anion and the supernatant of mouse liver homogenate after the ultracentrifugation were performed. The results obtained were as follows. 1. The relationships between the reductive rate of mercuric ion and the concentration of the (L)-AsA or (DL)-α-tocopherol were shown as a sigmoidal curve; that of the superoxide anion was a linear. 2. The activity of reduction of Hg(++) was in decreasing order with (L)-AsA, (DL)-α-tocopherol, and superoxide anion, respectively. 3. The liver homogenate was used with 8.5% sucrose and 11% polyvinylpirrolidone (PVP) solution with preparation of the homogenate to prevent release of catalase from the peroxisomes. Another preparation used 44mM phosphate buffer (pH 6.8) solution and the liver catalase was released from peroxisomes. The reductive rate of mercuric ion with the former preparation was 6.4 times higher than with the latter. It indicates that the reoxidation of metallic mercury by the catalase reduced the reductive rate of mercuric ion. 4. A reductive experiment of mercuric ion by liver supernatant containing sucrose and PVP at 0°C and 37°C was performed. The reduction rate by this sample at 0°C is 1/5.48 of that at 37° C. This suggests that the reduction of mercuric ion in liver homogenate is due to an enzymatic reaction.
en-copyright=
kn-copyright=

en-aut-name=KurahashiKouzirou
en-aut-sei=Kurahashi
en-aut-mei=Kouzirou
kn-aut-name=倉橋康二郎
kn-aut-sei=倉橋
kn-aut-mei=康二郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=reduction of mercuric ion
kn-keyword=reduction of mercuric ion
en-keyword=(L)-ascorbic acid
kn-keyword=(L)-ascorbic acid
en-keyword=(DL)-α-tocopherol
kn-keyword=(DL)-α-tocopherol
en-keyword=superoxide anion
kn-keyword=superoxide anion
en-keyword=liver homogenate
kn-keyword=liver homogenate
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=589
end-page=602
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Abnormality of dominant oncogenes and recessive oncogenes in human bone and soft tissue tumors
kn-title=ヒト骨・軟部腫瘍における優性および劣性癌遺伝子の変異に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Osteosarcoma is a malignant bone tumor which usually occurs in the metaphysis of long bones in adolescents or young adults, but the mechanism of disease occurrence is unknown. To examine alterations in the dominant oncogenes in the osteosarcoma and other bone and soft tissue tumors, DNA extracted from 12 bone tumors, 12 soft part tumors and 2 cells lines was hybridized with c-oncogene (c-myc, c-sis, c-raf-1, c-fos, K-ras2, c-erbB, c-fms, c-fos). DNA extracted from the same samples was examined with Rb cDNA (p0.9R, p3.8R) probes, the retinoblastoma gene (Rb gene) localized at 13q14, to analize whether a recessive mutation is a target in osteosarcoma, and other bone and soft tissue tumors. Amplification of c-myc was observed in 3 cases from 7 osteosarcomas and abnormalities in structure of the Rb gene were found in 4 from 7 osteosarcomas, 2 from 4 MFH, and 1 from 2 Ewing's sarcomas. Both amplification of the c-myc and abnormalities of the Rb gene were observed in 3 osteosarcoma cases. The results indicated that both the Rb gene and c-myc oncogene may be involved in the initiation of osteosarcoma.
en-copyright=
kn-copyright=

en-aut-name=OzakiToshifumi
en-aut-sei=Ozaki
en-aut-mei=Toshifumi
kn-aut-name=尾崎敏文
kn-aut-sei=尾崎
kn-aut-mei=敏文
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部附属癌源研究施設生化学研究部門
en-keyword=骨・軟部腫瘍
kn-keyword=骨・軟部腫瘍
en-keyword=優性癌遺伝子
kn-keyword=優性癌遺伝子
en-keyword=劣性癌遺伝子
kn-keyword=劣性癌遺伝子
en-keyword=Rb遺伝子
kn-keyword=Rb遺伝子
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=581
end-page=588
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies of the treatment of pulmonary epidermoid carcinoma Part 2. Thermochemotherapy in human epidermoid carcinoma cells in culture
kn-title=肺扁平上皮癌の治療に関する研究 第2編 ヒト肺扁平上皮癌細胞株を用いた温熱化学療法に関する検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The effectiveness of hyperthermia in combination with anticancer drugs on EBC-1 cell line established from a patient with pulmonary epidermoid carcinoma was investigated. Anticancer drugs tested in the present study were adriamycin, bleomycin, cisdichlorodiammineplatinum (II) and mitomycin C. EBC-1 cells were incubated with the drug for one hour at 37°C or at elevated temperature (41°C, 42°C and 43°C). The enhancement of cytotoxicity by hyperthermia was found in combination with all of the drugs. The degree of enhanced cytotoxicity was positively elated to the temperature in combinations with adriamycin and bleomycin. Especially, a synergic enhancement of cytotoxicity was found with the combination of hyperthermia and bleomycin. The present study showed that the combination of hyperthermia and anticancer drugs produced potential cytotoxicity on EBC-1 cells in vitro, and may provide useful information for the clinical trials in the future.
en-copyright=
kn-copyright=

en-aut-name=SetoTakumi
en-aut-sei=Seto
en-aut-mei=Takumi
kn-aut-name=瀬戸匠
kn-aut-sei=瀬戸
kn-aut-mei=匠
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=human lung cancer cell line
kn-keyword=human lung cancer cell line
en-keyword=thermochemotherapy
kn-keyword=thermochemotherapy
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=573
end-page=580
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies of the treatment of pulmonary epidermoid carcinoma Part 1. Sensitivity to various anticancer drugs against human pulmonary epidermoid carcinoma xenograft in the hamster
kn-title=肺扁平上皮癌の治療に関する研究 第1編 ハムスター移植ヒト肺扁平上皮癌細胞株に対する各種制癌剤の感受性について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Chemotherapy is the only therapeutic modality applicable to patients with advanced pulmonary epidermoid carcinoma (PEC). However, the results of chemotherapy to PEC remain unsatisfactory. It is very important to have an accurate knowledge of the sensitivity of anticancer drugs against PEC in order to establish a successful chemotherapy. The in vivo sensitivity of 12 anticancer drugs was investigated using PEC cell line (EBC-1) xenografts in hamsters.
In the present study, adriamycin, ifosphamide, mitomycin C, methotrexate and cisdichlorodiammineplatinum (II) showed antitumor activity against EBC-1 cells, but the other 6 drugs (ACNU, bleomycin, 5-fluorouracil, neocarzinostatin, procarbazine and vincristine) have no antitumor activity against EBC-1 cells. These results suggest that combination chemotherapy with 3 or 4 drugs with antitumor activity in the present study may be effective to PEC.
en-copyright=
kn-copyright=

en-aut-name=SetoTakumi
en-aut-sei=Seto
en-aut-mei=Takumi
kn-aut-name=瀬戸匠
kn-aut-sei=瀬戸
kn-aut-mei=匠
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=human lung cancer cell line
kn-keyword=human lung cancer cell line
en-keyword=xenograft
kn-keyword=xenograft
en-keyword=chemosensitivity test
kn-keyword=chemosensitivity test
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=557
end-page=571
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Molecular cloning and structural analyses of human DNA sequences related to the squirrel monkey retrovirus LTR
kn-title=リスザルレトロウイルスLTR関連ヒト遺伝子の分子クローニングと構造解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Sequences related to the squirrel monkey retrovirus (SMRV) LTR were isolated from a genomic library of human DNA by screening under conditions of relaxed stringency. The probe contains several transcriptional signals and its regulatory sequences in SMRV-H LTR, such as the enhancer sequence, CAT box, TATA box, and polyadenylation signal. More than 50 positive signals were detected in 4.4×105 recombinant phages. The cloned human sequences named SRH strongly hybridized with SMRV-H LTR and some of them weakly hybridized with SMRV-H structural genes. In SRH-1, the region related to SMRV-H prt-pol-env was flanked by the two separately located sequences related to the LTR. SRH-3 has a solitary LTR-related region, whereas, in SRH-5, the LTR-related region was adjacent to the pol-env-related sequence. The reliability of these hybridization experiments was confirmed by reciprocal hybridization. The restriction enzyme cleavage maps of these clones were different from those of known human endogenous retroviruses. Alu sequences, a human highly repetitive sequences, were inserted to the LTR-and pol-env-related regions in SRH-5.
en-copyright=
kn-copyright=

en-aut-name=NagaoKazutaka
en-aut-sei=Nagao
en-aut-mei=Kazutaka
kn-aut-name=長尾一孝
kn-aut-sei=長尾
kn-aut-mei=一孝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌源研究施設生化学部門
en-keyword=ヒト内在性レトロウイルス
kn-keyword=ヒト内在性レトロウイルス
en-keyword=リスザルレトロウイルス
kn-keyword=リスザルレトロウイルス
en-keyword=Long terminal repeat
kn-keyword=Long terminal repeat
en-keyword=分子クローニング
kn-keyword=分子クローニング
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=541
end-page=555
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The effect of keyboard height during VDT work on local muscular load
kn-title=VDT作業時のキーボード高と肩腕部筋負担に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Electromyograms (EMG) during Japanese and English word processing work with various keyboard heights was recorded and analyzed with amplitude probability distribution methods, in order to examine the effect of keyboard height on local muscular load in the shoulder and forearm regions. The EMG was recorded from the extensor carpi radialis brevis muscles, the trapezius muscles and anterior and medial portion of the deltoideus muscles. The keyboard height was placed at 5cm below elbow, elbow height, 10cm above elbow and height selected by the examinee. Eight young female students volunteered to participate in this experimental study. According to the results of the EMG amplitude probability distribution analysis, keyboard height gave different loads to the respective muscles. For example, the load to the extensor carpi radialis brevis muscles decreased as the keyboard position became higher, while the load of trapezius muscles increased. In conclusion, it was suggested that there is no best keyboard height during VDT work from the view point of muscles load. Therefore the keyboard height should be adjusted considering the characteristics of individual VDT work and the muscles fatigue of the operator.
en-copyright=
kn-copyright=

en-aut-name=OtaniToru
en-aut-sei=Otani
en-aut-mei=Toru
kn-aut-name=大谷透
kn-aut-sei=大谷
kn-aut-mei=透
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部衛生学教室
en-keyword=筋電図
kn-keyword=筋電図
en-keyword=APD分析法
kn-keyword=APD分析法
en-keyword=肩腕部筋負担
kn-keyword=肩腕部筋負担
en-keyword=VDT作業
kn-keyword=VDT作業
en-keyword=キーボード高
kn-keyword=キーボード高
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=529
end-page=540
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Malignant neoplasms among patients with certified chronic arsenic poisoning in Toroku
kn-title=土呂久における慢性ヒ素中毒認定患者の悪性新生物についての疫学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=An epidemiological study has been made to assess the carcinogenic effect of arsenic exposure by analyzing a group of patients with certified chronic arsenic poisoning at “Toroku”, a small mountainous village in Japan. The number of deaths from cancer of the respiratory system and cancer of bladder, kidney and other and unspecified urinary organs showed a significant excess over the expected value, compared with expected number of deaths, based on sex and age-specific mortality of Japanese in 1975, 80 and 85. The number of patients from all cancers, respiratory cancer and urinary tract cancer showed a significant exess of former workers, using data classified by employment history. Classified by employment history and smoking habits, the number of respiratory cancers showed a significant excess only in the cohort of former workers with a smoking history. This finding suggests that there is an interaction between exposure to arsenic and smoking. Eleven of seventeen cancer cases were accompanied by cancer of other organs, including Bowen's disease. These results demonstrated that a follow-up study on these patients should be conducted with a particular emphasis on cancers.
en-copyright=
kn-copyright=

en-aut-name=TsudaToshihide
en-aut-sei=Tsuda
en-aut-mei=Toshihide
kn-aut-name=津田敏秀
kn-aut-sei=津田
kn-aut-mei=敏秀
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部脳代謝研究施設病態生化学部門
en-keyword=ヒ素
kn-keyword=ヒ素
en-keyword=産業中毒
kn-keyword=産業中毒
en-keyword=疫学
kn-keyword=疫学
en-keyword=悪性新生物
kn-keyword=悪性新生物
en-keyword=公害
kn-keyword=公害
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=517
end-page=528
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental study of circulating catecholamines on contractility and oxygen consumption in excised cross-circulated dog heart
kn-title=内因性，外因性カテコルアミンのイヌ摘出交叉灌流心臓の収縮性および酸素消費量に及ぼす影響に関する実験的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author studied the effects of circulating catecholamines (CA) on both the left ventricular (LV) contractility index (Emax) and the relationship between LV O(2) consumption (VO(2)) and systolic pressure-volume area (PVA) in 11 excised cross-circulated dog heart preparations. Adrenal medullary stimulation (AMS) of the support dog caused frequencydependent increases in circulating CA. The correlation between the increases in Emax and CA was statistically significant in each heart preparation, but the sensitivity of Emax to CA varied widely. The VO(2)-PVA data point under fixed LV end-diastolic and stroke volumes moved linearly right-upward with increases in Emax. The slope of the VO(2)-PVA locus was steeper than the slope of the reference VO(2)-PVA relationship in control contractility. An infusion of exogenous epinephrine produced similar results to that obtained in the AMS run. The present results indicated a similarity between the effects of endogenous and exognenous catecholamines on both Emax and the elevation of the VO(2)-PVA relation in each heart preparation with a variability in the sensitivity of the elevation of the VO(2)-PVA relationship varied widely among the individual hearts.
en-copyright=
kn-copyright=

en-aut-name=OhgoshiYuichi
en-aut-sei=Ohgoshi
en-aut-mei=Yuichi
kn-aut-name=大越祐一
kn-aut-sei=大越
kn-aut-mei=祐一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二外科学教室
en-keyword=心機能
kn-keyword=心機能
en-keyword=酸素消費量
kn-keyword=酸素消費量
en-keyword=カテコルアミン
kn-keyword=カテコルアミン
en-keyword=副腎
kn-keyword=副腎
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=505
end-page=515
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Test for susceptibility to anticancer drugs by human tumor clonogenic assay and anticancer effects of natural human tumor necrosis factor-α and natural human interferon-α
kn-title=Human tumor clonogenic assayを用いた制癌剤感受性試験とnatural human Tumor Necrosis Factorの抗腫瘍効果に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The human tumor clonogenic assay is a test of susceptibility to anticancer drugs which is known to correlate well with clinical effects. In this report, this assay was used to study the antitumor effects of several anticancer drugs and human tumor necrosis factor-α (nHuTNF-α), and the combination effect of nHuTNF-α and natural human interferon-α (nHuIFN-α). Susceptibility to exisiting anticancer drugs was studied in 94 cases involving 92 patients. Colony formation was obtained 50 cases (53.2%). Of these, susceptibility to ADM was found in 15 cases, to MMC in 8 cases and to 5-Fu in 11 cases. When the antitumor effect of nHuTNF-α was studied in 10 cases obtained from patients with malignant tumor, colony inhibition was shown in 9 cases of more than 30% with 100 units and more than 50% with 300 units. A synergistic effect was assessed with a combination of nHuTNF-α and nHuIFN-α against 4 cell lines (KB, Hep-2, KATO-III, PC-9). The results showed a synergistic effect in KB and Hep-2 cell culture system. From these results, nHuTNF-α seemed to be effective anticancer drugs. The synergistic antitumor effect may be expected if the combination of nHuTNF-α and nHuIFN-α was used clinically.
en-copyright=
kn-copyright=

en-aut-name=OkamotoKanji
en-aut-sei=Okamoto
en-aut-mei=Kanji
kn-aut-name=岡本幹司
kn-aut-sei=岡本
kn-aut-mei=幹司
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科学教室
en-keyword=Human tumor clonogenic assay
kn-keyword=Human tumor clonogenic assay
en-keyword=抗癌剤感受性試験
kn-keyword=抗癌剤感受性試験
en-keyword=natural human interferon-α
kn-keyword=natural human interferon-α
en-keyword=natural human tumor necrosis factor-α
kn-keyword=natural human tumor necrosis factor-α
en-keyword=preclinical early phase II study
kn-keyword=preclinical early phase II study
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=495
end-page=503
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Metallothionein messenger RNA regulation in the macular mutant mouse: An animal model of Menkes's kinky-hair disease
kn-title=Menkes症モデルマウス (Macular mouse) でのMetallothionein mRNAの調節
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=MT-mRNA levels in tissue were measured in normal and macular mutant mice. The MT-mRNA level in tissues (kidney, intestine, liver, brain, spleen, thymus, lung and heart) of 7-day-old mutants was not changed compared to normal mice. Kidney MT-mRNA levels in the mutant foetus at 18 days of gestation and in the 3- and 7-day-old mutant were not changed compared compared to normal mice, with the exception of the 1-day-old mutant. Intestine and liver MT-mRNA levels in the mutant foetus at 18 days gestation and in the 1-, 3- and 7-day-old mutant were not changed compared to normal mice.
en-copyright=
kn-copyright=

en-aut-name=KajiMitsumasa
en-aut-sei=Kaji
en-aut-mei=Mitsumasa
kn-aut-name=加地充昌
kn-aut-sei=加地
kn-aut-mei=充昌
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部放射線医学教室
en-keyword=Menkes's kinky-hair disease
kn-keyword=Menkes's kinky-hair disease
en-keyword=Metallothionein mRNA
kn-keyword=Metallothionein mRNA
en-keyword=Copper
kn-keyword=Copper
en-keyword=Kidney
kn-keyword=Kidney
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=487
end-page=494
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Echocardiography in congenital pericardial defect - Alterations of cardiac motion induced by posture changes -
kn-title=先天性心膜欠損症における心臓超音波所見（体位変換による所見の変化）
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The true incidence of congenital pericardial defects is unknown, but it is more common than generally supposed. Because of the variance of chest X-rays, ECG and symptoms according to the degree of the defect, it is still not easy to diagnose a pericardial defect. Recently, we experienced four patients with congenital pericardial defects. Three cases were absence of the left pericardium and one was an absence of the right. In these patients, the view with echocardiography was characteristically altered by posture changes. All three patients with left-sided defect showed an enlarged right ventricular cavity, paradoxycal motion of the interventricular septum and hyperkinetic motion of the posterior wall in the left lateral decubitus position. These findings were not present in the right decubitus position. On the other hand, in a case with a right-sided defect, the view was almost normal in the left decubitus position. In the right decubitus position, the right ventricular cavity enlarged and the interventricular septum moved hyperkineticaly. These alterations of echocardiographic findings by posture changes were specific to the side of the pericardial defect, and were caused by a change in cardiac restraint. Therefore, an echocardiogram recorded with posture changes is useful in the diagnosis of congenital pericardial defects.
en-copyright=
kn-copyright=

en-aut-name=HinaKazuyoshi
en-aut-sei=Hina
en-aut-mei=Kazuyoshi
kn-aut-name=日名一誠
kn-aut-sei=日名
kn-aut-mei=一誠
aut-affil-num=1
ORCID=

en-aut-name=UeedaMasayuki
en-aut-sei=Ueeda
en-aut-mei=Masayuki
kn-aut-name=上枝正幸
kn-aut-sei=上枝
kn-aut-mei=正幸
aut-affil-num=2
ORCID=

en-aut-name=UchidaShinji
en-aut-sei=Uchida
en-aut-mei=Shinji
kn-aut-name=内田真司
kn-aut-sei=内田
kn-aut-mei=真司
aut-affil-num=3
ORCID=

en-aut-name=HasuiMasahiro
en-aut-sei=Hasui
en-aut-mei=Masahiro
kn-aut-name=蓮井雅浩
kn-aut-sei=蓮井
kn-aut-mei=雅浩
aut-affil-num=4
ORCID=

en-aut-name=MimaTsutomu
en-aut-sei=Mima
en-aut-mei=Tsutomu
kn-aut-name=美馬敦
kn-aut-sei=美馬
kn-aut-mei=敦
aut-affil-num=5
ORCID=

en-aut-name=WatanabeHirofumi
en-aut-sei=Watanabe
en-aut-mei=Hirofumi
kn-aut-name=渡辺博史
kn-aut-sei=渡辺
kn-aut-mei=博史
aut-affil-num=6
ORCID=

en-aut-name=SaitoDaiji
en-aut-sei=Saito
en-aut-mei=Daiji
kn-aut-name=斉藤大治
kn-aut-sei=斉藤
kn-aut-mei=大治
aut-affil-num=7
ORCID=

en-aut-name=TsujiTakao
en-aut-sei=Tsuji
en-aut-mei=Takao
kn-aut-name=辻孝夫
kn-aut-sei=辻
kn-aut-mei=孝夫
aut-affil-num=8
ORCID=

en-aut-name=MatubaraKatashi
en-aut-sei=Matubara
en-aut-mei=Katashi
kn-aut-name=松原堅
kn-aut-sei=松原
kn-aut-mei=堅
aut-affil-num=9
ORCID=

en-aut-name=YoshidaHidenori
en-aut-sei=Yoshida
en-aut-mei=Hidenori
kn-aut-name=吉田英紀
kn-aut-sei=吉田
kn-aut-mei=英紀
aut-affil-num=10
ORCID=

en-aut-name=HaraokaShoichi
en-aut-sei=Haraoka
en-aut-mei=Shoichi
kn-aut-name=原岡昭一
kn-aut-sei=原岡
kn-aut-mei=昭一
aut-affil-num=11
ORCID=

en-aut-name=IwasakiKohichiro
en-aut-sei=Iwasaki
en-aut-mei=Kohichiro
kn-aut-name=岩崎孝一郎
kn-aut-sei=岩崎
kn-aut-mei=孝一郎
aut-affil-num=12
ORCID=

en-aut-name=KusachiShozo
en-aut-sei=Kusachi
en-aut-mei=Shozo
kn-aut-name=草地省蔵
kn-aut-sei=草地
kn-aut-mei=省蔵
aut-affil-num=13
ORCID=

en-aut-name=KitaToshimasa
en-aut-sei=Kita
en-aut-mei=Toshimasa
kn-aut-name=喜多利正
kn-aut-sei=喜多
kn-aut-mei=利正
aut-affil-num=14
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=2
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=3
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=4
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=5
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=6
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=7
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=8
en-affil=
kn-affil=岡山大学医学部第一内科学教室

affil-num=9
en-affil=
kn-affil=岡山大学医学部臨床検査医学教室

affil-num=10
en-affil=
kn-affil=岡山大学医学部臨床検査医学教室

affil-num=11
en-affil=
kn-affil=岡山大学医学部臨床検査医学教室

affil-num=12
en-affil=
kn-affil=心臓病センター榊原病院

affil-num=13
en-affil=
kn-affil=心臓病センター榊原病院

affil-num=14
en-affil=
kn-affil=心臓病センター榊原病院
en-keyword=心膜欠損症
kn-keyword=心膜欠損症
en-keyword=心臓超音波
kn-keyword=心臓超音波
en-keyword=心室中隔奇異性運動
kn-keyword=心室中隔奇異性運動
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=473
end-page=488
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Evaluation of methemoglobin formation from hemoglobin in the blood of normal and acatalasemic mice using nitric oxide or nitric dioxide
kn-title=アカタラセミアマウスに一酸化窒素，二酸化窒素曝露時のメトヘモグロビン生成
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The formation of methemoglobin from hemoglobin in the hemolysates and erythrocytes of normal and acatalasemic mice was studied by exposure to nitric oxide and nitric dioxide. The data indicated that the ratio of methemoglobin formation in acatalasemic mice is larger than that in the normal mice in every case, and nitric oxide has a stronger action compared to nitric dioxide in regard to methemoglobin formation. The results indicated that hemoglobin is changed to methemoglobin through nitrosylhemoglobin with nitric oxide, and catalase has an inhibiting action on this oxidation process.
en-copyright=
kn-copyright=

en-aut-name=IshiiKunihiko
en-aut-sei=Ishii
en-aut-mei=Kunihiko
kn-aut-name=石井邦彦
kn-aut-sei=石井
kn-aut-mei=邦彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=NO
kn-keyword=NO
en-keyword=NO(2)
kn-keyword=NO(2)
en-keyword=カタラーゼ
kn-keyword=カタラーゼ
en-keyword=メトヘモグロビン
kn-keyword=メトヘモグロビン
en-keyword=アカタラセミア
kn-keyword=アカタラセミア
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=459
end-page=471
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Immunoelectron microscopic studies of human immunodeficiency virus antigens
kn-title=ヒト免疫不全ウイルス抗原の免疫電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Immunoelectron microscopic observations of human immunodeficiency virus (HIV) antigens were made using the indirect peroxidase-labeled antibody method with monoclonal antibodies (MoAb) to the envelope glycoprotein (gp160/120), matrix protein (MA, p17), and capsid protein (CA, p24). Antigens in the virions and HIV-infected cells were detected in frozen-sectioned specimens. Positive immunoreaction of MoAb to gp160/120 was located on the viral envelope, plasma membrane, endoplasmic reticulum, and nuclear membrane. The reaction of MoAb to p17 was at the inner leaflet of the viral envelope and the nucleoid. The reaction of MoAb to p24 was localized on and around the viral core. In cell suspension, however, positive immunostaining was observed only on the surface of the viral envelope and plasma membrane with MoAb to gp160/120, and no positive staining was observed with MoAb to p17 and MoAb to p24. The reaction of HIV-infected cells with normal control serum and all of the reactions in uninfected cells with these MoAbs were negative. It appears that antibodies have difficulty penetrating into virions and cells in cell suspension.
en-copyright=
kn-copyright=

en-aut-name=EndoSatoru
en-aut-sei=Endo
en-aut-mei=Satoru
kn-aut-name=遠藤悟
kn-aut-sei=遠藤
kn-aut-mei=悟
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌源研究施設生化学部門
en-keyword=ヒト免疫不全ウイルス
kn-keyword=ヒト免疫不全ウイルス
en-keyword=HIV抗原
kn-keyword=HIV抗原
en-keyword=構造蛋白質
kn-keyword=構造蛋白質
en-keyword=免疫電顕
kn-keyword=免疫電顕
en-keyword=酵素抗体法
kn-keyword=酵素抗体法
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=447
end-page=457
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Evaluation of the representativeness of a continuous monitoring station for air pollution from nitrogen dioxide in an urban area
kn-title=市街地大気汚染測定局の地域代表性に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to evaluate the representativeness of a monitoring station for nitrogen dioxide in an urban area of Okayama City, the nitrogen dioxide concentration was measured in the area six times between 1982 and 1984. The area was divided into 96 sections and the concentration of nitrogen dioxide was measured at the central part of each section and at a continuous monitoring station located in the area. From the measured values, the distribution of nitrogen dioxide concentration levels was determined and the correlation coefficients of the levels at the measuring points were calculated. The results show that the nitrogen dioxide concentration levels vary according to the direction and strength of wind, and other factors, but the levels of nitrogen dioxide at the continuous monitoring station were close to the mean level of the 96 measuring points throughout all observation days. The correlation coefficients between the nitrogen dioxide levels at the continuous monitoring station and those of the other 96 measuring points were large. It can be concluded that the present continuous monitoring station is one of the best points to monitor nitrogen dioxide concentration in the urban area of Okayama City.
en-copyright=
kn-copyright=

en-aut-name=FujiwaraFukuichi
en-aut-sei=Fujiwara
en-aut-mei=Fukuichi
kn-aut-name=藤原福一
kn-aut-sei=藤原
kn-aut-mei=福一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部衛生学教室
en-keyword=nitrogen dioxide
kn-keyword=nitrogen dioxide
en-keyword=representativeness
kn-keyword=representativeness
en-keyword=correlation coefficient
kn-keyword=correlation coefficient
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=437
end-page=446
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Analysis of cadmium resistant mechanisms of Lactobacillus
kn-title=Lactobacillusにおけるカドミウム耐性機構の解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The cadmium resistance of Lactobacillus and its mechanism were examined, with the following results: (1) The 16 strains of Lactobacillus were classified into two groups: 6 strains which were sensitive to cadmium and 10 strains which were resistant to more than 1mM cadmium. (2) All cadmium sensitive strains belonged to L. plantarum and L. fermentum species, and all cadmium resistant strains were L. casei and L. acidophilus species. (3) The barrier to cadmium influx in cadmium resistant strains showed no difference between L. casei and L. acidophilus strains. (4) The cytoplasmic cadmium contents in L. casei were higher than those in L. acidophilus, and there was a significant difference between L. casei and L. acidophilus (P<0.01). (5) From these results, L. casei appears to possess a mechanism for the detoxication of cytoplasmic cadmium using cadmium binding proteins and L. acidophilus appears to have a mechanism for pumping out cytoplasmic cadmium.
en-copyright=
kn-copyright=

en-aut-name=KatayamaYasuo
en-aut-sei=Katayama
en-aut-mei=Yasuo
kn-aut-name=片山靖夫
kn-aut-sei=片山
kn-aut-mei=靖夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部細菌学教室
en-keyword=Cadmium
kn-keyword=Cadmium
en-keyword=Lactobacillus
kn-keyword=Lactobacillus
en-keyword=Resistance
kn-keyword=Resistance
en-keyword=Detoxication
kn-keyword=Detoxication
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=5-6
article-no=
start-page=423
end-page=435
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1989
dt-pub=198906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Detection of human sequence related to the squirrel monkey retrovirus
kn-title=ヒトゲノムDNA中のリスザルレトロウイルス関連遺伝子の検出
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Squirrel monkey retrovirus (SMRV) is an endogenous type D retrovirus of the squirrel monkey, a New World primate. Southern hybridization with cloned SMRV-H revealed that 3040 copies of SMRV proviral DNA are present in the squirrel monkey genome and the majority have almost the same physical map as that of the cloned SMRV-H. SMRV-related sequences in the human genome were sought using the same method with various cloned SMRV-H DNA fragments under conditions of relaxed stringency. The discrete restriction fragments were frequently detected in the DNA from normal humans with the LTR and parts of gag and env as probes. Since SMRV LTR has very little homology with the LTRs of other retroviruses, the fragments detected with the LTR probe were characterized as SMRV-related human sequences. SMRV LTR-related sequences were also detected in the African green monkey and chicken, but not in the salmon, mouse, or dog. In conclusion, SMRV-related sequences are present in human DNA, and some of them might represent endogenous retroviral sequences of human DNA.
en-copyright=
kn-copyright=

en-aut-name=AsonumaHirohiko
en-aut-sei=Asonuma
en-aut-mei=Hirohiko
kn-aut-name=阿曽沼裕彦
kn-aut-sei=阿曽沼
kn-aut-mei=裕彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌源研究施設生化学部門
en-keyword=ヒトDNA
kn-keyword=ヒトDNA
en-keyword=内在性レトロウイルス
kn-keyword=内在性レトロウイルス
en-keyword=リスザルレトロウイルス
kn-keyword=リスザルレトロウイルス
en-keyword=サザンハイブリダイゼーション
kn-keyword=サザンハイブリダイゼーション
END