start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=1075 end-page=1079 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of beautiful bone scan due to bone metastasis of gastric cancer kn-title=Beautiful Bone Scanを呈した胃癌骨転移の1例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 46-year-old female presented with lumbago. A plain X-ray examination did not indicate any distinctive changes, however a bone scintigram showed beautiful bone scan. A bone marrow biopsy certified metastatic adenocarcinoma and a primary gastric carcinoma was discovered subsequently. Beautiful bone scan can be seen in malignant metastasis, several bone metabolic diseases and other rare cases. A rare case of a gastric cancer exhibiting a beautiful bone scan, is described. en-copyright= kn-copyright= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name=黒田昌宏 kn-aut-sei=黒田 kn-aut-mei=昌宏 aut-affil-num=1 ORCID= en-aut-name=TanakaAkio en-aut-sei=Tanaka en-aut-mei=Akio kn-aut-name=田中朗雄 kn-aut-sei=田中 kn-aut-mei=朗雄 aut-affil-num=2 ORCID= en-aut-name=YamamotoYoshio en-aut-sei=Yamamoto en-aut-mei=Yoshio kn-aut-name=山本淑雄 kn-aut-sei=山本 kn-aut-mei=淑雄 aut-affil-num=3 ORCID= en-aut-name=SimizuMitsuharu en-aut-sei=Simizu en-aut-mei=Mitsuharu kn-aut-name=清水光春 kn-aut-sei=清水 kn-aut-mei=光春 aut-affil-num=4 ORCID= en-aut-name=SatoNobuo en-aut-sei=Sato en-aut-mei=Nobuo kn-aut-name=佐藤伸夫 kn-aut-sei=佐藤 kn-aut-mei=伸夫 aut-affil-num=5 ORCID= en-aut-name=JojaIkuo en-aut-sei=Joja en-aut-mei=Ikuo kn-aut-name=上者郁夫 kn-aut-sei=上者 kn-aut-mei=郁夫 aut-affil-num=6 ORCID= en-aut-name=HashimotoKeiji en-aut-sei=Hashimoto en-aut-mei=Keiji kn-aut-name=橋本啓二 kn-aut-sei=橋本 kn-aut-mei=啓二 aut-affil-num=7 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name=平木祥夫 kn-aut-sei=平木 kn-aut-mei=祥夫 aut-affil-num=8 ORCID= en-aut-name=NakadaHiroyuki en-aut-sei=Nakada en-aut-mei=Hiroyuki kn-aut-name=仲田浩之 kn-aut-sei=仲田 kn-aut-mei=浩之 aut-affil-num=9 ORCID= en-aut-name=KibataMasayoshi en-aut-sei=Kibata en-aut-mei=Masayoshi kn-aut-name=木畑正義 kn-aut-sei=木畑 kn-aut-mei=正義 aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=8 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=9 en-affil= kn-affil=国立療養所南岡山病院内科 affil-num=10 en-affil= kn-affil=国立療養所南岡山病院内科 en-keyword=Beautiful Bone Scan kn-keyword=Beautiful Bone Scan en-keyword=胃癌 kn-keyword=胃癌 en-keyword=骨転移 kn-keyword=骨転移 en-keyword=播種性骨髄癌症 kn-keyword=播種性骨髄癌症 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=1063 end-page=1073 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Changes in pituitary prolactin reserve with sexual maturation kn-title=性成熟に伴うprolactin分泌予備能の変動 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the factors affecting pituitary prolactin (PRL) reserve during development, the serum PRL response to TRH and serum LH, FSH response to LH-RH were studied. The basal levels of serum estradiol (E(2)), testosterone (T) and dehydroepiandrosterone-sulfate (DHEA-S) were measured in 128 normal children and adolescents and 18 cases of Turner's syndrome. In males, no correlation was found between E(2), T, DHEA-S and the integrated secretion of PRL (捻RL), while a positive correlation was found between the integrated secretion of FSH (認SH) and 捻RL (p<0.05). In females, positive correlations were found between E(2) and 捻RL (p<0.01), the integrated secretion of LH (猫H) and 捻RL (p<0.01) and DHEA-S and 捻RL (p<0.01). E(2) was selected as the explanatory variable of 捻RL by means of multiple regression analysis. In cases of Turner's syndrome, endocrinologically characterized by high gonadotropins and low E(2), a positive correlation was found between 認SH and 捻RL (p<0.05). These findings suggest a stimulating effect of E(2) on PRL reserve in females and the stimulating effect of endogenous LH-RH on PRL reserve in both sexes in children and adolescents. en-copyright= kn-copyright= en-aut-name=OmuraTsutomu en-aut-sei=Omura en-aut-mei=Tsutomu kn-aut-name=大村勉 kn-aut-sei=大村 kn-aut-mei=勉 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科学教室 en-keyword=prolactin分泌予備能 kn-keyword=prolactin分泌予備能 en-keyword=TRH kn-keyword=TRH en-keyword=LH-RH負荷試験 kn-keyword=LH-RH負荷試験 en-keyword=性ホルモン kn-keyword=性ホルモン en-keyword=性成熟 kn-keyword=性成熟 en-keyword=Turner症候群 kn-keyword=Turner症候群 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=1049 end-page=1061 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Thermal damage threshold of brain tissue - Histological study of heated normal monkey brains - kn-title=正常脳組織の加温限界の検討―温熱によるサル正常脳組織の組織学的変化― en-subtitle= kn-subtitle= en-abstract= kn-abstract=The thermal damage threshold of brain tissue was estimated from the immediate and delayed histological changes caused by 2450 MHz microwave antenna and an antenna cooling system of a device used for interstitial hyperthermia treatment. An antenna within a cooling system was inserted through the small cranietomy under general anesthesia. The temperature at a reference point, 4 mm radially away from the surface of the cooling system, was maintained at 42 °C, 43 °C, 44 °C, 45 °C or 46 °C for 60 minutes. In a non-survival experiment, 18 animals were used and sacrificed immediately after the treatment. In a survival experiment, 9 animals were used and sacrificed seven days after the treatment. The histological changes were evaluated by microscopic examination with hematoxylin and eosin, Kl?ver-Barrera, or Bodian stainings. In the non-survival experiment, areas heated below 44 °C showed no obvious irreversible change. In the survival experiment, areas heated at 44 °C or above showed coagulative necrosis. Those histological findings indicate that the thermal damage occurs in normal brain tissue after heating at 44 °C or above for 60 minutes, and suggest that the safety limit for brain hyperthermia is 43 °C for 60 minutes. en-copyright= kn-copyright= en-aut-name=MatsumiNobuhiko en-aut-sei=Matsumi en-aut-mei=Nobuhiko kn-aut-name=松海信彦 kn-aut-sei=松海 kn-aut-mei=信彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳神経外科学教室 en-keyword=hyperthermia kn-keyword=hyperthermia en-keyword=interstitial microwave irradiation kn-keyword=interstitial microwave irradiation en-keyword=histological change kn-keyword=histological change en-keyword=malignant brain tumors kn-keyword=malignant brain tumors en-keyword=antenna cooling system kn-keyword=antenna cooling system END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=1037 end-page=1048 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The origin of liver epithelial-like cells derived from the normal rat kn-title=正常ラット肝由来上皮様細胞の起源 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To investigate the origin of liver epithelial-like cells derived from the normal rat, we examined ten liver epithelial-like cell clones. These cultured cells were isolated from clonogenically growing cells in the primary culture. Morphological, cytochemical and biochemical characteristics of these ten of clones showed the followings: (1) These cells consisted of dense, diffuse and intermediate types. (2) Analyses of serum-free conditioned culture media, using SDS-PAGE, demonstrated that the dense type had a certain profile in the group of secretory proteins, whereas the diffuse and intermediate types had varied profiles although the latter were somewhat similar in profile to the dense type. (3) The secretory protein groups were different from mesenchymal cell lines in their profiles. (4) Cytokeratin was present in all the ten of clones. (5) Cytokeratin was also present in the cholangiolar epithelia and cells localized in peripheral areas of normal hepatic lobules. (6) The function, as seen in mature-type hepatocytes and cholangiolar epithelia, was almost absent. (7) These ten clones were different from sinusoidal endothelia according to their morphologies and growth ability in the primary culture. In conclusion, the liver epithelial-like cell clones derived from normal rat can belong to a “stem-cell family” of liver tissue. These “stem cells” may be localized next to the hepatocytes and cholangiolar epithelia in vivo. en-copyright= kn-copyright= en-aut-name=MatsuuraKazuhiko en-aut-sei=Matsuura en-aut-mei=Kazuhiko kn-aut-name=松浦一彦 kn-aut-sei=松浦 kn-aut-mei=一彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設病理部 en-keyword=ラット kn-keyword=ラット en-keyword=肝細胞培養 kn-keyword=肝細胞培養 en-keyword=上皮様細胞 kn-keyword=上皮様細胞 en-keyword=cytokeratin kn-keyword=cytokeratin en-keyword=stem cell kn-keyword=stem cell END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=1027 end-page=1036 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of IgG bound to basophils in bronchial asthmatics - A role of IgG subclass in late asthmatic response - kn-title=気管支喘息患者好塩基球表面のIgG結合に関する研究 第2編 遅発型気道反応におけるIgGサブクラスの役割について en-subtitle= kn-subtitle= en-abstract= kn-abstract=There are many problems in intractable asthmatics, such as corticosteroid dependency. The clinical course is different from atopic asthma. To clarify the differences between both type of asthma, we investigated the mechanism of LAR, which has similar features to the pathogenesis of intractable asthma. We have previously reported that IgG antibody was involved with the LAR. In this study, to clarify the role of IgG subclass, we observed the density and distribution of IgG1 and IgG4 antibodies bound to the peripheral blood basophils from bronchial asthmatics using a technique of immunoscaning electron microscopy. A study revealed a relative ratio of IgG1 to IgG4 antibodies on basophils of asthmatics with Candida-provoked LAR which was higher than that of asthmatics with house dust-provoked IAR and LAR. Furthermore, in the study of immunoglobulin distribution on the basophil surface, the proportion of cap and patch formation of IgG1 antibody was higher in bronchial asthmatics with Candida-provoked LAR than in those with house dust-provoked IAR and LAR. These data suggest that IgG1 antibody may play an important role in the pathogenesis of LAR, especially Candida-provoked asthmatic response. en-copyright= kn-copyright= en-aut-name=OkadaChiharu en-aut-sei=Okada en-aut-mei=Chiharu kn-aut-name=岡田千春 kn-aut-sei=岡田 kn-aut-mei=千春 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=late asthmatic response kn-keyword=late asthmatic response en-keyword=IgG kn-keyword=IgG en-keyword=subclass kn-keyword=subclass en-keyword=basophil kn-keyword=basophil END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=1015 end-page=1025 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of IgG bound to basophils in bronchial asthmatics - A role of IgG antibody in late asthmatic response - kn-title=気管支喘息患者好塩基球表面のIgG結合に関する研究 第1編 遅発型気道反応におけるIgG抗体の関与について en-subtitle= kn-subtitle= en-abstract= kn-abstract=The similarities between the clinical characteristics of instractable asthma and attacks of late asthmatic response (LAR) have been noted. To clarify the pathogenesis of intractable asthma, the mechanism of LAR was studied using a technique of immuno-scaning electron microscopy to determine the density and distribution of immunoglobulins bound to the surface of peripheral blood basophils from bronchial asthma patients with asthmatic responses provoked by inhalation of house dust or Candida antigens. In a study of immunoglobulin density, the ratio of the number of IgG to IgE antibodies was higher in asthmatics with house dust-provoked LAR than in those with IAR, and the values were even higher in those with Candida-provoked LAR. In a study of the distribution of bound immunoglobulins, the overall rates of cap and patch formations, which seems to indicate an activation of cells, tended to be higher in asthmatics with house dust-provoked LAR than in those with IAR. The same tendency was also seen in those with Candida-provoked LAR. These results suggest that the IgG antibodies play an important role in LAR, although IgE antibodies are predominant in IAR. This trend is marked in asthmatics with Candida-induced LAR. en-copyright= kn-copyright= en-aut-name=OkadaChiharu en-aut-sei=Okada en-aut-mei=Chiharu kn-aut-name=岡田千春 kn-aut-sei=岡田 kn-aut-mei=千春 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=late asthmatic response kn-keyword=late asthmatic response en-keyword=IgE kn-keyword=IgE en-keyword=IgG kn-keyword=IgG en-keyword=basophil kn-keyword=basophil en-keyword=immuno-scanning electron microscopy kn-keyword=immuno-scanning electron microscopy END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=1009 end-page=1014 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ultrastructure of the “open secretion” of beta-cell granules from Langhans' islets in Fe(3+)-NTA-treated diabetic rats kn-title=Fe(3+)-NTA投与糖尿病ラットのβ細胞開口分泌の電顕的観察 en-subtitle= kn-subtitle= en-abstract=The secretion time of endocrine cells is known to be momentary. Therefore, morphologic proof of the secretory mode have relied heavily on various animal models which were treated to accelerate secretion. Ultrastructure, concerning B-cell secretion from Langhans' islets of the pancreas, has been based on immersion-fixation specimens. As an experimental model of prolonged endocrine secretion, we used rat pancreas tissues which were rapidly perfused with a fixative. The fixation process, as reported previously, was 150 ml of 2% glutaraldehyde flushed at 15 ml per minute through the ascending aorta of Fe(3+)-NTA-treated diabetic rats. Afterwards, an ultrastructural study focused on the secreting mode of beta-granules in B-cell of Langhans' islets. This procedure proved an “open secretion” of beta-granules from B-cells; this phenomenon has never been observed in the previous immersion-fixation specimens. Contrary to non-treated normal rats, the varied stages of beta-granule secretion, including immediately before as well as after the secretion, were demonstrated in Fe(3+)-NTA-treated rats. We wish to emphasize the use of rapid perfusion-fixation technique in animal models with a delayed rather than accelerated state of endocrine secretion in order to investigate ultrastractural aspects of “open secretion”. kn-abstract=内分泌腺細胞の分泌時間は瞬時とされていることから,一般的にその分泌像の形態的観察には,分泌を促進せしめる実験系が用いられている.又膵ラ氏島B細胞分泌の超微形態は,専ら浸漬固定標本で観察されている.今回,我々は分泌が遅延している動物で,高速還流した膵ラ氏島細胞について観察した.即ち,前報と同様のFe(3+)-NTA誘発糖尿病ラットの上行大動脈より2%グルタールアルデヒド150mlを15ml/minの速度で還流固定し,ラ氏島B細胞のβ顆粒分泌像に焦点を絞って観察した.その結果,前報の浸漬固定法では観察されなかった開口分泌像が多数観察され得た.対照の健常動物に比べ, Fe(3+)-NTA投与動物では,分泌直前から分泌後に至るいろいろな段階のβ顆粒の分泌過程が観察された.以上の結果から,開口分泌像の電顕的観察にはむしろ本実験系のように細胞分泌を障害ないし低下せしめ,高速還流固定法を用いることが必要であると結論した. en-copyright= kn-copyright= en-aut-name=HayashiKeiki en-aut-sei=Hayashi en-aut-mei=Keiki kn-aut-name=林肇輝 kn-aut-sei=林 kn-aut-mei=肇輝 aut-affil-num=1 ORCID= en-aut-name=ShirasugaNaoki en-aut-sei=Shirasuga en-aut-mei=Naoki kn-aut-name=白須賀直樹 kn-aut-sei=白須賀 kn-aut-mei=直樹 aut-affil-num=2 ORCID= en-aut-name=OnoToshiro en-aut-sei=Ono en-aut-mei=Toshiro kn-aut-name=小野俊郎 kn-aut-sei=小野 kn-aut-mei=俊郎 aut-affil-num=3 ORCID= en-aut-name=AwaiMichiyasu en-aut-sei=Awai en-aut-mei=Michiyasu kn-aut-name=粟井通泰 kn-aut-sei=粟井 kn-aut-mei=通泰 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部病理学第一講座 affil-num=2 en-affil= kn-affil=岡山大学医学部病理学第一講座 affil-num=3 en-affil= kn-affil=岡山大学医学部病理学第一講座 affil-num=4 en-affil= kn-affil=岡山大学医学部病理学第一講座 en-keyword=Diabetes mellitus kn-keyword=Diabetes mellitus en-keyword=β-cell's granules kn-keyword=β-cell's granules en-keyword=open secretion kn-keyword=open secretion en-keyword=perfusion fixation kn-keyword=perfusion fixation END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=999 end-page=1007 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Heat stability of catalase activity in the blood and liver of acatalasemic mice Part 2. Heat stabilty of catalase activity and changes in the serum enzyme activities after oral administration of ethyl chlorophenoxy isobutyrate kn-title=アカタラセミアマウスの血液および肝臓カタラーゼ活性の熱安定性 第2編 clofibrate投与時のアカタラセミアマウス肝臓カタラーゼ活性の熱安定性および血清酵素活性の変動 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Normal and acatalasemic mice were fed 0.5% clofibrate (CPIB) for 70 days and variations of catalase activity and thermal stability of liver catalase were investigated. 1. The specific catalase activity (catalase activity/g liver weight) in the liver of mice which received CPIB compared to that of controls was calculated. The specific catalase activity was in 2.2 normal mice and 1.2 in acatalasemic mice. A significant increase of activity was recognized in normal mice but not in acatalasemic mice. 2. The specific catalase activity in the blood of mice which received CPIB to that of control was determined. The specific catalase activity was 1.3 in normal mice and 1.1 in acatalasemic mice. A significant increase was not observed in either kind of mice. 3. The heat stability of catalase in the liver of normal or acatalasemic mice which received with CPIB was higher than that of normal or acatalasemic mice which did not received CPIB. 4. Increased levels of ALP and ChE activities and decreased levels of GOT activity and TG concentration were observed in the normal or acatalasemic mice which received CPIB, compared to control mice. 5. Isozyme of ALP were determined by electrophoresis. Increased ALP in the plasma of normal and acatalasemic mice was thought to be derived from liver ALP. en-copyright= kn-copyright= en-aut-name=KogashiwaMichiko en-aut-sei=Kogashiwa en-aut-mei=Michiko kn-aut-name=小柏道子 kn-aut-sei=小柏 kn-aut-mei=道子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=アカタラセミアマウス kn-keyword=アカタラセミアマウス en-keyword=clofibrate kn-keyword=clofibrate en-keyword=カタラーゼ kn-keyword=カタラーゼ en-keyword=熱安定性 kn-keyword=熱安定性 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=991 end-page=997 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Heat stability of catalase activity in the blood and liver of acatalasemic mice Part 1. Heat stabilty of catalase activity after administration of phenylhydrazine hydrochloride subcutaneously kn-title=アカタラセミアマウスの血液および肝臓カタラーゼ活性の熱安定性 第1編 フェニルヒドラジン投与時のアカタラセミアマウス血液カタラーゼ活性の熱安定性 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to investigate the heat stability of catalase in immature and mature erythrocytes of normal, acatalasemic and heterozygous hypocatalasemic mics, phenylhydrazine hydrochloride (PHZ) was administered to these mice at a dose of 40 μg/g of body weight subcutaneously daily for 3 days Anemic blood rich in reticulocytes was obtained 2 days after final injection. Thermostability of catalase in the non-anemic and anemic bloods was compared and the following results were obtained. 1. Specific catalase activity (catalase activity/amount of hemoglobin; PU/g Hb) in anemic blood from acatalasemic mice was significantly higher than that in non-anemic blood. Specific catalase activity in anemic blood from normal mice was a little higher than that in non-anemic blood from normal mice. 2. The residual catalase activity in the hemolysate after heat treatment was compared with that from before treatment in which hemolysate containing catalase was incubated at 42, 44, 46, 48 and 50 °C for 10 minutes in a water bath. At any temperature, catalase activity in immature red blood cells of normal and acatalasemic mice was more stable with heat than that inmature red blood cells. 3. In mormal mice, the T(50) (the temperature at which 50 % of the catalase activity remained after heat treatment) in anemic normal blood was statistically higher than that in non-anemic blood. In the acatalasemic mice, the T(12.5) (the temperature at which 12.5 % of the catalase activity remained after heat treatment) in anemic blood was statistically higher than that in non-anemic blood. en-copyright= kn-copyright= en-aut-name=KogashiwaMichiko en-aut-sei=Kogashiwa en-aut-mei=Michiko kn-aut-name=小柏道子 kn-aut-sei=小柏 kn-aut-mei=道子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=アカタラセミアマウス kn-keyword=アカタラセミアマウス en-keyword=フェニルヒドラジン kn-keyword=フェニルヒドラジン en-keyword=カタラーゼ kn-keyword=カタラーゼ en-keyword=熱安定性 kn-keyword=熱安定性 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=11-12 article-no= start-page=977 end-page=989 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of guanidinoethanesulfonic acid induced convulsions - Effect of guanidinoethanesulfonic acid on monoamines in the mouse brain - kn-title=Guanidinoethanesulfonic acid誘発痙攣に関する研究―特に脳内モノアミンにおよぼす影響― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Guanidinoethanesulfonic acid (GES) is known to induce convulsive seizures when administered intracisternally into rabbits and cats. I examined the effects of GES on behavior, electroencephalogram and brain monoamine levels after intraventricular injection into mice. When GES was intraventricularly injected into mice, focal clonic movements of the face, vibrissae and ears, as well as twitchings of limbs were observed 0.5-1 min after injection. Hypersensitivity was observed until 7 min after the injection, after which the mice behaved in a normal way. It is also observed that GES induced sporadic spike discharges in the electroencephalogram. The latency and duration of the appearence of spike discharges showed considerable individual variation. The latency was from 0 to 5 min, and the duration was from 5 to 90 min. No spike discharge was observed in the saline injected animals. The 5-hydroxytryptamine (5-HT) level decreased in the hippocampus, diencephalon, pons-medulla oblongata and cerebellum 5 min after injection, and recovered to the control level 10 min after the injection. No change in the norepinephrine (NE) and dopamine (DA) levels was found after GES injection. 5-hydroxyindoleacetic acid increased in the striatum and cerebellum 5 min after injection. It is thought that the GES injection enhances the release of 5-HT in the striatum and cerebellum, although the mechanism of the decrease of 5-HT in the hippocampus, diencephalon and pons-medulla oblongata is unclear. These results indicate that the GES induced convulsive seizure is related to 5-HT neurons, not NE or DA neurons. en-copyright= kn-copyright= en-aut-name=WatanabeShunji en-aut-sei=Watanabe en-aut-mei=Shunji kn-aut-name=渡辺駿二 kn-aut-sei=渡辺 kn-aut-mei=駿二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳代謝研究施設機能生化学部門教室 en-keyword=guanidinoethanesulfonic acid kn-keyword=guanidinoethanesulfonic acid en-keyword=convulsion kn-keyword=convulsion en-keyword=brain monoamines kn-keyword=brain monoamines en-keyword=5-hydroxytryptamine kn-keyword=5-hydroxytryptamine END