| Title Alternative | Odontoglossum Ringspot Virus Isolated from Calanthe sp. in Japan |
|---|---|
| FullText URL | 003_002_163_174.pdf |
| Author | Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | A rod-shape virus isolated from Calanthe sp. showing chlorotic mottle on the leaves, collected in Ibaraki Prefecture, was identified as odontoglossum ringspot virus(ORSV). The isolate,designated as Cal.92-1T, was transmitted by sap-inoculation to 14 out of 40 species in 6 out of 12 families. The virus particles were rod-shaped, about 310 nm long. In ultrahtin sections, the dispersed and aggregated virus particles were observed in the cytoplasm of the cells of the infected leaves of Chenopodium quinoa. The virus contained a single protein species of Mr 20,600. The Mr of the capsid proteins(Cal.92-1T) was similar to those of three other ORSV isolates(Cy-1,Cy-46,Cy-Kei). Cal.92-1T isolate(Cy-1), suggesting that Cal.92-1T was serologically very similar to the other ORSV isolates. Three species of dsRNA were isolated from plants infected with Cal.92-1T and they were similar to those of three other ORSV isolates. |
| Keywords | Odontoglossum ringspot virus Calanthe sp. Identification |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 163 |
| End Page | 174 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Host Range and Some Properties of Orchid Fleck Virus Isolated form Oriental Cymbidium in Japan |
|---|---|
| FullText URL | 003_002_151_161.pdf |
| Author | Kondo, Hideki| Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | Orchid flck virus (OFV) was isolated from Oriental Cymbidium (Cymbidium sp.),showing chlorotic flecks on leaves. The virus was transmitted mechanically to Chenopodium quinoa,C.murale and Beta vulgaris by sap-inoculation and caused systemic infection. Local lesions were produced on C.amaranticolar, Petunia hybrida, Tetragonia expansa and Vigna sinensis. Sap from infected T.expansa was still infective after 10 mim at 40℃ but not after 10 min at 45℃, at a dilution of 10-3 but not 10-4, and after 30 min at room temperature but not after 60 min. The isolate of OFV had non-enveloped, bullet-shaped patricles measuring about 40×120－150 nm in dip preparations. However, bacilliform particles about 40×120-140 nm were observed in ultrathin sections. In ultrahtin sections of virus-infected tissues, virus patricles were detected both in the nuclei and in the cytoplasm. Moreover, the inclusions of low electron density(viroplasm) were also observed in the nuclei.Virus particles were found to attach at one end to the inner nuclear membrane. A number of particles surrounded by the inner membrane often showed an appearance like a spoked wheel. |
| Keywords | Orchid fleck virus Oriental Cymbidium Non-enveloped rhabdovirus like particles |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 151 |
| End Page | 161 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313940 |
| Title Alternative | Cloning of PCR-Products Encoding Potassium Channel Proteins from Mesembryanthemum crystallinum |
|---|---|
| FullText URL | 003_002_145_149.pdf |
| Author | Katsuhara, Maki| Bohnert, Hans J.| |
| Abstract | Gene fragments of potassium channels were cloned from Mesembryanthemum crystallinum by using RT-PCR (reverse transcription-polymerase chain reaction). The two fragments were isolated independently and showed high similarity with each other. About 80% identity was found between the two fragments and potassium-channel genes of Arabidopsis. Southern hybridization indicated that the potassium channel gene may be a single copy gene or that a small gene family of potassium channels exists. |
| Keywords | Mesembryanthemum crystallinum Potassium channel RT-PCR |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 145 |
| End Page | 149 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification |
|---|---|
| FullText URL | 003_002_137_143.pdf |
| Author | Akiyama, Yoshiko| Shibasaka, Mineo| Kawasaki, Toshio| |
| Abstract | Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged. |
| Keywords | Adenylate extraction ADP AMP ATP Barley roots |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 137 |
| End Page | 143 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Effects of Mannosen on Hydroponically Grown Barley |
|---|---|
| FullText URL | 003_002_129_135.pdf |
| Author | Shibasaka, Mineo| Miyata, Masahiko| Akiyama, Yoshiko| Kawasaki, Toshio| |
| Abstract | To examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants. |
| Keywords | Barley Hydroponics Mannose Potassium Phosphate |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 129 |
| End Page | 135 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Differences in the Responses to Iron Deficiency Stress between Bean and Maize |
|---|---|
| FullText URL | 003_002_117_127.pdf |
| Author | Yonetani, Tsutomu| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. |
| Keywords | Bean Iron deficiency stress Iron reducing capacity Maize Medium-pH lowering capacity |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 117 |
| End Page | 127 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Effect of Light Irradiation on the Absorption of Rubidium in Chlorella |
|---|---|
| FullText URL | 003_002_105_116.pdf |
| Author | Hachiya, Kinji| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | The effect of light irradiation on the absorption of rubidium was examined in Chlorella. Rubidium absorption in Chlorella was clearly stimulated by the irradiation of light. To clarify the mechanism of light-stimulation on rubidium absorption, experiments were carried out using several metabolic inhibitors; DCMU, NaCN,DNP,CCCP and ouabain. Among the metabolic inhibitors used, DCMU had the most similar effects on the rubidium absorption and oxygen evolution under light condition. These findings suggest a close correlation between the light-stimulation on rubidium absorption and the photosynthetic process in Chlorella. |
| Keywords | Chlorella Light-stimulation Metabolic inhibitors Oxygen evolution Rubidium absorption |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 105 |
| End Page | 116 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | 数種植物におけるアンモニウムイオン及び硝酸イオンの吸収速度の比較 |
|---|---|
| FullText URL | 003_002_091_103.pdf |
| Author | Moritsugu, Masumi| Kawasaki, Toshio| Suzuki, Takao| |
| Abstract | Absorption rares of ammonium ion(NH4+) and nitrate ion(NO3-) for 24 hours were compared using two absorption solutions, which were a single salt solution of ammonium nitrate (NH4NO3) and a complete nutrient solution containing NH4NO3. Test plants were Oryza sativa (Rice), Hordeum vulgare (Barley), Lactuca sativa (Lettuce), Cucumis sativus (Cucumber), Daucus carota (Carrot), Brassica pekinesis (Chinese cabbage), Spinacia oleracea (Spinach) and Raphanus sativus (Radish). From the absorption characteristics of NH4+ and NO3- between a single salt solution of NH4NO3 and a complete nutrient solution containing NH4NO3, the test plants were classified into goup 1(rice,barley and lettuce), which absorbed NH4+ more rapidly than NO3- in both absorption solutions, group 2(cucumber and carrot), which absorbed NH4+ slightly more than NO3- in the single salt solution of NH4NO3, the tendency of which was reversed in the complete nutrient solution containing NH4NO3, and goup 3(Chinese cabbage,spinach and radish), which absorbed NO3- clealy more than NH4+ in the complete nutrient solution, whereas the absorption of NH4+ or NO3- was almost equal in the single salt solution of NH4+NO3-. The above classisication of plants could be explained by the balance of a repressive or competitive characteristics of NH4+ absorption mainly as sociated with a capacity for absorption of calcium ion(Ca2+) and magnesium ion(Mg2+), and the relative root affinity to NO3- that can be evaluated by the relative absorption of NO3- to mono-phosphate ion(H2PO4-) in plant roots. The group 1 plants are the so-called acid tolerant plants, which appeared to be tolerant to NH4+, and showed a marked pH decreased during the 24 hours of the absorption experiments in those plants. By contrast, the group 3 plant seemed to prefer NO3- and divalent alkaline earth cations to the other nutrients in the complete nutrient solution containing NH4No3, and the pH decrease during each absorption experiment was small. |
| Keywords | Alkaline earth Ammonium Ion balance Nitrate Phosphate |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 091 |
| End Page | 103 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
