start-ver=1.4
cd-journal=joma
no-vol=1
cd-vols=
no-issue=2
article-no=
start-page=100053
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200613
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Protocol for Genome Editing to Produce Multiple Mutants in Wheat
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for mutant production. The mutants produced by this protocol can be used for the analysis of gene function and crop improvement. For complete details on the use and execution of this protocol, please refer to Abe et?al. (2019).
en-copyright=
kn-copyright=

en-aut-name=AbeFumitaka
en-aut-sei=Abe
en-aut-mei=Fumitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IshidaYuji
en-aut-sei=Ishida
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HisanoHiroshi
en-aut-sei=Hisano
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=EndoMasaki
en-aut-sei=Endo
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=KomariToshihiko
en-aut-sei=Komari
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TokiSeiichi
en-aut-sei=Toki
en-aut-mei=Seiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=SatoKazuhiro
en-aut-sei=Sato
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Division of Basic Research, Institute of Crop Science, NARO
kn-affil=

affil-num=2
en-affil=Plant Innovation Center, Japan Tobacco Inc.
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=4
en-affil=Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
kn-affil=

affil-num=5
en-affil=Plant Innovation Center, Japan Tobacco Inc.
kn-affil=

affil-num=6
en-affil=Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
kn-affil=

affil-num=7
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=
END