start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=3
article-no=
start-page=358
end-page=364
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201003
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Improved development of mouse and human embryos using a tilting embryo culture system
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression and friction force in the Fallopian tube before nidation. In order to apply mechanical stimuli to embryos in a conventional IVF culture system, the tilting embryo culture system (TECS) was developed. The observed embryo images from the TECS suggest that the velocities and shear stresses of TECS embryos are similar to those experienced in the oviduct. Use of TECS enhanced the development rate to the blastocyst stage and significantly increased the cell number of mouse blastocysts (P < 0.05). Although not statistically significant, human thawed embryos showed slight improvement in development to the blastocyst stage following culture in TECS compared with static controls. Rates of blastocyst formation following culture in TECS were significantly improved in low-quality embryos and those embryos cultured under suboptimal conditions (P < 0.05). The TECS is proposed as a promising approach to improve embryo development and blastocyst formation by exposing embryos to mechanical stimuli similar to those in the Fallopian tube.
en-copyright=
kn-copyright=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HayashiNobuyoshi
en-aut-sei=Hayashi
en-aut-mei=Nobuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurodaYuka
en-aut-sei=Kuroda
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakiueChisato
en-aut-sei=Takiue
en-aut-mei=Chisato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HirataRei
en-aut-sei=Hirata
en-aut-mei=Rei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakenamiMami
en-aut-sei=Takenami
en-aut-mei=Mami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=AoiYoko
en-aut-sei=Aoi
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YoshiokaNanako
en-aut-sei=Yoshioka
en-aut-mei=Nanako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=HabaraToshihiro
en-aut-sei=Habara
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MukaidaTetsunori
en-aut-sei=Mukaida
en-aut-mei=Tetsunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=3
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=4
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=5
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=6
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=7
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=8
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=9
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=10
en-affil=
kn-affil=Hiroshima HART Clinic

affil-num=11
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=blastocyst
kn-keyword=blastocyst
en-keyword=embryo development
kn-keyword=embryo development
en-keyword=mechanical stimuli
kn-keyword=mechanical stimuli
en-keyword=shear stress
kn-keyword=shear stress
en-keyword=tilting embryo culture system
kn-keyword=tilting embryo culture system
END