start-ver=1.4
cd-journal=joma
no-vol=7
cd-vols=
no-issue=11
article-no=
start-page=e50480
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130322
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca2+-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is La3+-dependent. Despite the absence of Ca2+ in the medium strain AM1 was able to grow on methanol in the presence of La3+. Addition of La3+ increased MDH activity but the addition had no effect on mxaF or xoxF1 expression level. We purified MDH from strain AM1 grown on methanol in the presence of La3+, and its N-terminal amino acid sequence corresponded to that of XoxF1. The enzyme contained La3+ as a cofactor. The ΔmxaF mutant strain could not grow on methanol in the presence of Ca2+, but was able to grow after supplementation with La3+. Taken together, these results show that XoxF1 participates in methanol metabolism as a La3+-dependent MDH in strain AM1.
en-copyright=
kn-copyright=

en-aut-name=NakagawaTomoyuki
en-aut-sei=Nakagawa
en-aut-mei=Tomoyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MitsuiRyoji
en-aut-sei=Mitsui
en-aut-mei=Ryoji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TaniAkio
en-aut-sei=Tani
en-aut-mei=Akio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SasaKentaro
en-aut-sei=Sasa
en-aut-mei=Kentaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TashiroShinya
en-aut-sei=Tashiro
en-aut-mei=Shinya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=IwamaTomonori
en-aut-sei=Iwama
en-aut-mei=Tomonori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=HayakawaTakashi
en-aut-sei=Hayakawa
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=KawaiKeiichi
en-aut-sei=Kawai
en-aut-mei=Keiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Faculty of Applied Biological Science, Gifu University

affil-num=2
en-affil=
kn-affil=Department of Biochemistry, Faculty of Science, Okayama University of Science

affil-num=3
en-affil=
kn-affil=Institute of Plant Science and Resources, Okayama University

affil-num=4
en-affil=
kn-affil=Faculty of Applied Biological Science, Gifu University

affil-num=5
en-affil=
kn-affil=Faculty of Applied Biological Science, Gifu University

affil-num=6
en-affil=
kn-affil=Faculty of Applied Biological Science, Gifu University

affil-num=7
en-affil=
kn-affil=Faculty of Applied Biological Science, Gifu University

affil-num=8
en-affil=
kn-affil=Tokai Gakuin University
END