start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=12 article-no= start-page=1122 end-page=1125 dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20251212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Sequence-Selective 2-O-Acetyl Modification of RNA Mediated by Duplex Formation with a Reactive Oligonucleotide Probe Incorporating 4-Thio-dT en-subtitle= kn-subtitle= en-abstract= kn-abstract=We designed and synthesized an oligonucleotide acetylating reagent (Ac-probe) that selectively acetylates the 2-OH groups of RNA upon forming a duplex with the target RNA. The Ac-probe can be readily prepared via a post-synthetic modification method using an oligodeoxynucleotide probe containing 4-thio-dT. During the acetylation reaction, 4-thio-dT is regenerated as the reaction proceeds. Notably, an efficient modification was observed when the complementary base of RNA to 4-thio-dT was cytosine or uracil, indicating the selectivity for the pyrimidine base. en-copyright= kn-copyright= en-aut-name=MuraseHirotaka en-aut-sei=Murase en-aut-mei=Hirotaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EtoMio en-aut-sei=Eto en-aut-mei=Mio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=LeeJeongsu en-aut-sei=Lee en-aut-mei=Jeongsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TaniguchiYosuke en-aut-sei=Taniguchi en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ImotoShuhei en-aut-sei=Imoto en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SasakiShigeki en-aut-sei=Sasaki en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Faculty of Pharmaceutical Sciences, Sojo University kn-affil= affil-num=2 en-affil=Faculty of Pharmaceutical Sciences, Sojo University kn-affil= affil-num=3 en-affil=Graduate School of Pharmaceutical Sciences, Nagasaki International University kn-affil= affil-num=4 en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=5 en-affil=Faculty of Pharmaceutical Sciences, Sojo University kn-affil= affil-num=6 en-affil=Graduate School of Pharmaceutical Sciences, Nagasaki International University kn-affil= en-keyword=RNA chemical modification kn-keyword=RNA chemical modification en-keyword=acetylation kn-keyword=acetylation en-keyword=site-specificity kn-keyword=site-specificity en-keyword=2-OH group kn-keyword=2-OH group END