start-ver=1.4
cd-journal=joma
no-vol=71
cd-vols=
no-issue=4
article-no=
start-page=405
end-page=416
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20211001
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Targeted genome modifications in cereal crops
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The recent advent of customizable endonucleases has led to remarkable advances in genetic engineering, as these molecular scissors allow for the targeted introduction of mutations or even precisely predefined genetic modifications into virtually any genomic target site of choice. Thanks to its unprecedented precision, efficiency, and functional versatility, this technology, commonly referred to as genome editing, has become an effective force not only in basic research devoted to the elucidation of gene function, but also for knowledgebased improvement of crop traits. Among the different platforms currently available for site-directed genome modifications, RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) endonucleases have proven to be the most powerful. This review provides an application-oriented overview of the development of customizable endonucleases, current approaches to cereal crop breeding, and future opportunities in this field.
en-copyright=
kn-copyright=

en-aut-name=HisanoHiroshi
en-aut-sei=Hisano
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=AbeFumitaka
en-aut-sei=Abe
en-aut-mei=Fumitaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HoffieRobert E.
en-aut-sei=Hoffie
en-aut-mei=Robert E.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KumlehnJochen
en-aut-sei=Kumlehn
en-aut-mei=Jochen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources, Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Crop Science, National Agriculture and Food Research Organization
kn-affil=

affil-num=3
en-affil=Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
kn-affil=

affil-num=4
en-affil=Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
kn-affil=
en-keyword=barley
kn-keyword=barley
en-keyword= CRISPR
kn-keyword= CRISPR
en-keyword= maize
kn-keyword= maize
en-keyword=rice
kn-keyword=rice
en-keyword=TALEN
kn-keyword=TALEN
en-keyword=wheat
kn-keyword=wheat
en-keyword=zinc-finger nucleases
kn-keyword=zinc-finger nucleases
END