start-ver=1.4
cd-journal=joma
no-vol=150
cd-vols=
no-issue=
article-no=
start-page=116001
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20219
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Odontoblast differentiation is regulated by an interplay between primary cilia and the canonical Wnt pathway
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Primary cilium is a protruding cellular organelle that has various physiological functions, especially in sensory reception. While an avalanche of reports on primary cilia have been published, the function of primary cilia in dental cells remains to be investigated. In this study, we focused on the function of primary cilia in dentin-producing odontoblasts. Odontoblasts, like most other cell types, possess primary cilia, which disappear upon the knockdown of intraflagellar transport-88. In cilia-depleted cells, the expression of dentin sialoprotein, an odontoblastic marker, was elevated, while the deposition of minerals was slowed. This was recapitulated by the activation of canonical Wnt pathway, also decreased the ratio of ciliated cells. In dental pulp cells, as they differentiated into odontoblasts, the ratio of ciliated cells was increased, whereas the canonical Wnt signaling activity was repressed. Our results collectively underscore the roles of primary cilia in regulating odontoblastic differentiation through canonical Wnt signaling. This study implies the existence of a feedback loop between primary cilia and the canonical Wnt pathway.
en-copyright=
kn-copyright=

en-aut-name=KawataKazumi
en-aut-sei=Kawata
en-aut-mei=Kazumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NaritaKeishi
en-aut-sei=Narita
en-aut-mei=Keishi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WashioAyako
en-aut-sei=Washio
en-aut-mei=Ayako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KitamuraChiaki
en-aut-sei=Kitamura
en-aut-mei=Chiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NishiharaTatsuji
en-aut-sei=Nishihara
en-aut-mei=Tatsuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KubotaSatoshi
en-aut-sei=Kubota
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TakedaSen
en-aut-sei=Takeda
en-aut-mei=Sen
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=2
en-affil=Department of Anatomy and Cell Biology, University of Yamanashi Interdisciplinary Graduate School of Medicine and Engineering
kn-affil=

affil-num=3
en-affil=Division of Endodontics and Restorative Dentistry, Department of Oral Functions, Kyushu Dental University
kn-affil=

affil-num=4
en-affil=Division of Endodontics and Restorative Dentistry, Department of Oral Functions, Kyushu Dental University
kn-affil=

affil-num=5
en-affil=Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University,
kn-affil=

affil-num=6
en-affil=Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=

affil-num=7
en-affil=Department of Anatomy and Cell Biology, University of Yamanashi Interdisciplinary Graduate School of Medicine and Engineering
kn-affil=
en-keyword=Primary cilia
kn-keyword=Primary cilia
en-keyword=IFT88
kn-keyword=IFT88
en-keyword=Odontoblast
kn-keyword=Odontoblast
en-keyword=Odontoblast differentiation
kn-keyword=Odontoblast differentiation
en-keyword=Canonical Wnt/β-catenin signaling pathway
kn-keyword=Canonical Wnt/β-catenin signaling pathway
END