Acta Medica Okayama2000Quantized vortex in Bose Einstein condensate of dilute atom gasesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000SENSITIVITY ANALYSIS IN SPATIAL STATISTICSENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Si-L2,3線を利用した金属silicide/Si接合系試料の定量分析ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000CELL-TO-CELL COMMUNICATION WITH GROWTH FACTORS IN THE MOUSE PITUITARY GLANDENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000希土類化合物のL端吸収･発光における磁気円二色性の理論ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000低温X線カメラの開発と有機一次元導体の研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000非線形励起による反強磁性体FeBO3のマイクロ波域磁気弾性波の研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Genetic and Molecular Analyses of Sexual Morphogenesis in Coprinus cinereusENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Optimal Linear Discriminant Functions using Mathematical ProgrammingENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Neural control of reproductive behavior in the male cricket Gryllus bimaculatus DeGeerENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Noble Gas Systematics and K-Ar Chronology: Implications for the Geochemical and Geotectonic Evolution of the Cameroon Volcanic Line, West Africa.ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000新規ベンズアミド系消化管運動機能改善薬TKS159の合成ならびに薬理学的研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000内分泌細胞におけるシナプス小胞様オルガネラ(マイクロベジクル)の構造と機能に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000変異原物質2-chloro-4-methylthiobutanoic acid に対するグリシンベタインの抗変異原性ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000新規免疫抑制剤創生のための標的分子としてのCD45 -プロテインチロシンフｫスフｧターゼ活性の調節機構の解明-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000新規アシルCoA : コレステロールアシルトランスフｪラーゼ阻害薬 NTE-122 の薬理学的特性に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000医薬品開発過程におけるビーグル犬を用いたトキシコキネテｨクスの薬物動態学的評価ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000共鳴ラマンスペクトルによる金属-TMPyP錯体とアルブミンの相互作用に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000細菌細胞における多剤排出系の生化学的及び遺伝学的解析ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000ポリクローナルIgE及びその誘導剤のNK細胞活性化とそのメカニズムの解析ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000銅フタロシアニン誘導体固定化担体を用いるステロイド化合物の高速液体クロマトグラフｨーに関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000大腸菌のmelibiose利用における新規温度感受性遺伝子発現制御系の発見とメカニズムの解析ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Ion Coupling in Secondary Transporters of BacteriaENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000ジクロロメタン分解菌の分離とその分解能力の検討ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000クインテｨリアヌス『弁論教程』第五巻注解-法的弁証の基礎的研究-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Moral Dilemmas of the Edwardian Middle Classes in E.M.Forster's NovelsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000The Main Thematic Current in John Steinbeck's Works:A Positive View of Man's SurvivalENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Null Subjects and Clause StructureENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000現代日本語の談話におけるフｨラーENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000John Steinbeck and His Dramatic World :A Study of His Dramatized Life,Works,and Adapted WorksENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000志賀文学における運命の超克ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000與謝野晶子研究-第一次『明星』期を中心に-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000｢正阿弥勝義と逸見東洋の研究｣-時代の変転が工芸に及ぼす影響について-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000イネの収量成立過程における耐塩性に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Aluminum Detoxification Mechanism by Organic Acids and Mucilage in Higher PlantsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Development of nitrogen application curve in spray chrysanthemums grown in hydroponic system and its practical use in NFT systemENHae sukYoonThe Graduate School of Natural Science and Technology, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000分子マーカーによるサトイモ(Colocasia esculenta (L.)Schott)とその近縁種における類縁関係の評価ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Molecular analysis of a gene encoding a putative myrosinase-binding protein specifically expressed in flowers of Arabidopsis thalianaENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000赤色酵母Rhodotorula minuta細胞膜エルゴステロールの分解と合成の光制御ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000STUDIES ON TUMOR NECROSIS FACTOR-α AND ITS RECEPTOR IN BOVINE CORPUS LUTEUMENRyosukeSakumotoThe objective of this study was to investigate the physiological roles of tumor necrosis factor α (TNFα) in bovine corpus luteum (CL) function throughout the estrous cycle and the entire gestation period. In the first series of experirnents, the expression of TNFα, the presence of functional TNFα receptors, and the expression of TNF receptor type I (TNF-RI) mRNA in the CL during different stages of the estrous cycle were examined. RT-PCR showed no difference in TNFα mRNA expression during the estrous cycle. Concentrations of TNFα in the CL tissue increased significantly from the mid- to the late luteal stage and decreased thereafter. A RT-PCR analysis showed higher levels of TNF -RI mRNA in CL of days 3-7 than in other stages. (125)I-TNFα binding to the membranes of bovine CL was maximal after incubation at 38 C for 48 h. The binding was much greater for TNFα than for related peptides. A Scatchard analysis revealed the presence of a high-affinity binding site in the CL membranes collected at each phase of the estrous cycle (dissociation constant (Kd); 3.60±0.58 - 5.79±0.19 nM). In contrast to TNF-RI mRNA expression, the levels of receptor protein were similar at each stage in the estrous cycle. When cultured cells of all luteal stages were exposed to TNFα (0.06-6 nM), TNFα stimulated prostaglandin (PG) F2α and PGE2 secretion by the cells in a dose-dependent fashion, especially during the early luteal phase, although it did not affect progesterone secretion. In the second series of experiments, the presence of TNFα mRNA and TNFα receptors in the bovine CL during the gestation period were investigated. The presence of TNFα mRNA and TNFα receptors on bovine CL from pregnant cows was investigated at three stages: trimesters I, II and III. TNFα mRNA was detected by an RT-PCR analysis in the CL of all stages of gestation. A Scatchard analysis revealed the presence of a high-affinity binding site (Kd; 5.1-6.9 nM) in the CL membranes collected at each stage of gestation. Furthermore, the concentrations of TNFα receptors in the CL of trimesters I (24.0± 1.95 pmol/mg protein) and III (21.6±2.39 pmol/mg protein) of gestation were significantly higher than the concentration in trimester II (14.9±2.07 pmol/mg protein). In the third series of experiments, I investigated the presence of functional TNFα receptors on the microvascular endothelial cells derived from developing bovine CL. TNFα receptors were analyzed by a radioreceptor assay using (125)I-labeled TNFα on two types of cultured endothelial cells. One has a cobblestone appearance (CS cells), and the other has a tube-like structure (TS cells). (125)I-labeled TNFα binding was maximal after incubation for 30 h at 37 C, and the specificity of binding was confinned. A Scatchard analysis showed the presence of two binding sites (high- and low- affinity) for TNFα receptors on both CS and TS cells. The Kd values and concentrations of the high-affinity binding sites for TNFα receptors were similar between CS and TS cells. However, Kd values and concentrations of the low-affinity binding sites in CS cells were significantly higher than those in TS cells. The expression of TNF-RI mRNA was determined in both cell types. Furthermore, TNFα
significantly stimulated PGE2 and endothelin-1 secretion by both CS and TS cells. The final series of experiments were conducted to clarify the intracellular signaling pathway of TNFα to stimulate PGF2α production in cultured bovine luteal cells. Bovine luteal cells that were obtained from mid- (days 8-12 after ovulation) CL were incubated with TNFα (0.6 nM) and/or various compounds as follows: U-73122 (a phospholipase (PL) C inhibitor), ACA (a PL-A2 inhibitor), H-89 (a protein kinase (PK) A inhibitor), calphostin C (a PK-C inhibitor), L-NAME/L-NORG (a nitric oxide synthase inhibitor), PD98059 (a mitogenactivated
protein kinase (MAPK) kinase inhibitor). U-73122 (0.1-10 μM), H-89 (0.1-10 μM), calphostin C (0.01-1 μM) and L-NAME/L-NORG (1-100 μM) did not affect TNFα-induced PGF2α secretion by the cultured cells. In contrast, ACA (1-100 μM) and PD98059 (0.1-100 μM) inhibited TNFα-stimulated PGF2α secretion by the cells in a dose-dependent fashion. The overall results in the present study indicate the local production of TNFα and the presence of functional TNF-RI in bovine CL throughout the estrous cycle and entire gestation period, and suggest that TNFα plays some roles as a paracrine factor in regulating bovine CL function. Furthermore, the present results indicate the presence of two types of TNF receptors and the expression of TNF-RI mRNA in the endothelial cells derived from bovine CL, suggesting TNFα plays two or more roles in regulating the secretory function of the endothelial cells. Finally, the present study also showed that the stimulatory effect of TNFα on PGs secretion by bovine luteal cells might be mediated via activation of the MAPK and PL-A2 pathways.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000食肉資源の有効利用と品質改善に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Ensiling characteristics and ruminal digestion of grass and legume silages treated with cell wall degrading enzymesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000植物防御遺伝子PSPAL1及びPSPAL2の発現制御に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000rhBMP-2/コラーゲン支持体における化学的固定化の有無による異所性骨誘導への影響についてENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Identification by Subtractive Hybridization of a Novel Insertion Sequence Specific for Virulent Strains of Porphyromonas gingivalisENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000口腔粘膜上皮異形成,上皮内癌および扁平上皮癌におけるEGFR蛋白過剰発現および遺伝子増幅に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Regulation of Apoptosis Reduction in the Cisplatin-Resistant A431 Cell Line by Bcl-2 and CPP32ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000歯科における行動科学 ーストレス認知が鼻部皮膚表面温度に及ぼす影響ーENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000ヒト口腔扁平上皮癌における浸潤･転移機構の解析-浸潤･転移能の異なる細胞株の樹立とE-cadherinのメチル化に関する検討-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000歯槽骨の創傷治癒時に発現する遺伝子に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000結合組織成長因子CTGF/ Hcs24の歯根膜線維芽細胞の増殖,分化に与える影響ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Actinobacillus actinomycetemcomitansの線毛のB細胞エピトープに関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000象牙質接着に応用するアミノ酸アクリレート含有セルフエッチングプライマーに関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000ラット新生仔における三叉神経1次求心ニューロンの細胞死ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Histomorphometric analysis on bone dynamics in denture supporting tissue under masticatory pressure in the ratENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000ブラッシングによるイヌ歯肉組織の応答範囲ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000持続的圧力による義歯床下骨組織の動態に関する組織計測的検討ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Zinc accumulation and metallothionein gene expression in the proliferating epidermis during wound healing in mouse skinENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000rhBMP-2/I型アテロコラーゲン複合体による異所性骨誘導の長期経過に関する病理組織学的研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Antibody responses against Porphyromonas gingivalis infection in the patients with early-onset periodontitisENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000三叉神経脊髄路核吻側亜核内に存在する侵害受容2次ニューロンENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000A histomorphometric analysis on bone dynamics in denture supporting tissue under continuous pressure in streptozotocin-induced diabetic ratENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000接着性コンポジットレジンセメントへのフッ素徐放機能の付与に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000骨移植における破骨細胞性骨吸収阻害剤ビスフｫスフｫネートの前処理による効果ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Studies on the Synthesis of Highly Functionalized Molecules through Chemo-Enzymatic MethodologyENKoichiMitsukuraNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Progressive Liquefaction and Densification Processes of Loosely Deposited Sand Bed under Oscillating Water PressureENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000高調波拡大現象の抑制を目的とした配電系統用アクテｨブフｨルタに関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Design of SAW Filters for their Transmission CharacteristicsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000ステッピングモータを用いた運動サーボ系に関する研究 -ステッピングモータ駆動平行二輪車の運動制御-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000密度依存を考慮した地下水および物質移動の数値解析に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000電力系統に設置するUPFCの制御法と過渡特性に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000生理活性ペプチド･タンパク質の高感度酵素免疫測定法の確立および生体機能解析への応用ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000高信頼性ソフトウｪア開発におけるテスト労力の最適配分問題に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000水熱処理によるゴミ焼却飛灰の有効利用に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Studies on the Synthesis of Functionalized Carbocycles Directed toward Natural Products and Related CompoundsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Synthesis and Artificial Functions of Mutant Proteins Incorporated with Nonnatural Amino AcidsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000A Study of Heat and Mass Transfer of a Falling Water Film, and Its Applications in an Open-Type Solar Thermal Energy Collector and Melting Falling SnowENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000抗血小板作用及び抗喘息作用を有するアミジノベンゾフラン誘導体の合成研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Characterization of Cysteine Synthetase Complex from Esherichia coli and Its Application to Synthetic ReactionENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Lipase-Catalyzed Synthesis of Pentafluorophenyl-Containing Cyanohydrin Leading to Novel Chiral LigandsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000圧電超音波アクチュエータの有限要素法援用設計に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000The Processing System for Eliminating Power-Line Interference in Biopotential SignalsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000近代日本近郊農村の生産と消費-1918年島根県下一集落の事例を中心に-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000磁気的残留応力測定法の精度向上に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000発展方程式の摂動と近似の理論ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Studies on ultratrace metal analysis by inductively coupled plasma -mass spectrometry in combination with flow injection and column treatmentENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000粉末焼結材の面圧強さに関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000新島襄の思想史的研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000看護職の職業性ストレスとキャリア発達に関する研究ENHikariInoshitaNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama20003次元異方的XYモデルの応用ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000近代における蚕糸業の展開の地域的特質-後発地域岡山県の場合-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Molecular biological studies on distribution of pathogenic factors of Vibrio mimicusENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000出土鉄製文化財の腐食と保存に関する材料科学的研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000創造的パフｫーマンスにおける課題に特有の拡散的思考訓練の効果についての実験的な分析-知能･創造性開発法を探る-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000知覚-運動学習における積極的休息および運動プログラム仮説に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000CHEMICAL STUDIES ON THE NEMATICIDAL CONSTITUENTS OF KNEMA HOOKERIANA (MYRISTICACEAE) AND BRUCEA SUMATRANA (SIMAROUBACEAE), A SUMATRAN RAINFOREST PLANTSENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000MOLECULAR GENETICAL STUDIES ON THE REGION SURROUNDING THE Tm-2 LOCUS IN TOMATOENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Plant defense responses and control of gene expression: Structure and function of the promoter of PSPAL2, a pea defensive gene encoding phenylalanine ammonia-lyaseENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Studies on the enzymes involved in the reduction of inorganic sulfur compounds in Thiobacillus ferrooxidansENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Estimation of unsaturated hydraulic parameters by in-situ techniqueENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Performance of wheat varieties under salt-affected conditions and role of mineral ions distribution in salt tolerance mechanismsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Genomic Structure of the Rat Major AP Endonuclease Gene(Apex) with an Adjacent Putative O-Sialoglycoprotease Gene (Prsmg1/Gcpl1) and a Processed Apex Pseudogene(Apexp1)ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Anti-HER2 antibody enhances the growth inhibitory effect of anti-oestrogen on breast cancer cells expressing both oestrogen receptors and HER2ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Growth inhibitory effects of ATP and its derivatives on human fibroblasts immortalized with 60Co-γ raysENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Methamphetamine decreases calcium-calmodulin dependent protein kinase U activity in discrete rat brain regionsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodideENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Poxvirus virions: their surface ultrastructure and interaction with the surface membrane of host cellsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Prevention of Halothane-Induced Hepatotoxicity by Hemin Pretreatment : The Protective Role of Heme Oxygenase-1 InductionENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000The treatment of port-wine stains by the dye laser: A study of 644 patientsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Indispensable role of the transcription factor PEBP2 /CBF in angiogenic activity of a murine endothelial cell MSS31ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Effect of Pilsicainide,a Pure Sodium Channel Blocker,on Spiral Waves During Atrial Fibrillation:Theoretical Analysis by Numerical SimulationENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Monitoring of Human Herpesvirus-6 and-7 Genomes in Saliva Samples of Healthy Adults by Competitive Quantitative PCRENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Electrophoretic and serologic characterization of 56 kDa antigen (M56) with autologous serum derived from a chondrosarcoma patient:A shared antigen of immunoresponses in cancer and autoimmune diseasesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Time-dependent Changes of Decorin in the Infarct Zone After Experimentally Induced Myocardial Infarction in Rats:Comparison with BiglycanENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Down-regulation of the Insulin-like Growth Factor I Receptor by Antisense RNA Can Reverse the Transformed Phenofield name="type" of Human Cervical Cancer Cell LinesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Influence of Exercise on Muscle Fibers in Rats with Steroid MyopathyENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Transforming Activity of the RL-akt Gene, a c-akt Gene Activated by Long Terminal Repeat Insertion in Murine Leukemia RL♂1CellsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000老人保健施設が老人医療費に与える影響についてENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Modulation of norepinephrine release by ATP-dependent K+-channel activators and inhibitors in guinea-pig and human isolated right atriumENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000FAS-MEDIATED CYTOTOXICITY BY γδ T CELLS DURING ACUTE REJECTION IN XENOTRANSPLANTATION OF SPHEROIDAL AGGREGATE-CULTURED HEPATOCYTESENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Forensic Study of Sex Determination using PCR on Teeth SamplesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Utility of MRI in detecting obstacles to reduction in developmental dysplasia of the hip:comparison with two-directional arthrography and correlation with intraoperative findingsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Recombinant Adenovirus Expressing Wild-Type p53 Is Antiangiogenic:A Proposed Mechanism for Bystander EffectENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000尿中落下逸脱細胞におけるTGF-βmRNA発現の検討ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Prognostic Value of Vascular Endothelial Growth Factor Expression in Primary Lung CarcinomaENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Expression of Cell Cycle Regulator p27kip1 Is Correlated with Survival of Patients with AstrocytomaENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Perineuronal Sulfated Proteoglycans and Cell Surface Glycoproteins in the Visual Cortex of Adult and Newborn CatsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Possible influences of Staphylococcus aureus on atopic dermatitis the colonizing features and the effects of staphylococcal enterotoxinsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000RELATIONSHIP BETWEEN p21/WAF-1/CIP-1 AND APOPTOSIS IN CERVICAL CANCER DURING RADIATION THERAPYENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Different expression of positive and negative regulators of hepatocyte growth in growing and shrinking hepatic lobes after portal vein branch ligation in ratsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000超音波画像による大腿四頭筋の形態評価 -厚さ測定の信頼性と臨床的有用性について-ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Experimental Evaluation of the Effects of the Intraportal Administration of Cyclic Guanosine Monophosphate on Ischemia / Reperfusion in the Porcine LiverENHideoMatsumotoNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Evaluation of Cyclin D1 mRNA Expression in Gastric and Colorectal CancersENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000INVOLVEMENT OF APOPTOSIS IN ACTIVATION-INDUCED CELL DEATH OF BACTERIA-REACTIVE HUMAN CD45RO+T CELLSENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Effects of Ca2+ and Epinephrine on Ca2+ Recirculation Fraction and Total Ca2+ Handling in Canine Left VentriclesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive ratsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Increase in the Expression of Biglycan mRNA Expression Co-localized Closely with that of Type I Collagen mRNA in the Infarct Zone After Experimentally-Induced Myocardial Infarction in RatsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000EFFECTIVE ARTERIAL ELASTANCE(Ea)OF IRREGULAR BEATS DURING ATRIAL FIBRILLATION IN CANINE LEFT VENTRICLEENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000A Novel Gene(Oculomedin)Induced by Mechanical Stretching in Human Trabecular Cells of the EyeENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Temporal Profile of Cytochrome c and Caspase-3 Immunoreactivities and TUNEL staining after Permanent Middle Cerebral Artery Occlusion in RatsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Cell Death and Proliferation and Its Relation to Collagen Degradation in Uterine Involution of RatENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Cytogenetic characteristics and p53 gene status of human teratocarcinoma PA-1 cells in 407-445 passagesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Attenuation of Conduction Delay by lschemic Preconditioning Reduces lschemia-Induced Ventricular ArrhythmiasENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Expression of CD44 variants in osteosarcomaENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000The effect of calcium-sensing receptor gene polymorphisms on serum calcium levels :a familial hypocalciuric hypercalcemia part name="family" without mutation in the calcium-sensing recept or geneENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000In vivo Evaluation of Bone-bonding of Titanium Metal Chemically Treated with a Hydrogen Peroxide Solution Containing Tantalum ChlorideENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Manumycin A,Inhibitor of ras Farnesyltransferase,Inhibits Proliferation and Migration of Rat Vascular Smooth Muscle CellsENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000慢性関節リウマチ膝に行った鏡視下滑膜切除術のMRIによる評価ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Oxyhemoglobin-induced apoptosis in cultured endothelial cellsENKotaroOgiwaraOBJECT: Oxyhemoglobin (OxyHb) is one of the most important spasmogens for cerebral vasospasm that follows aneurysmal subarachnoid hemorrhage. The cytotoxic effect of OxyHb has been documented in endothelial and smooth-muscle cells; however, the pattern of cell death--necrosis or apoptosis--as the final stage of cell damage has not been demonstrated. This study was undertaken to determine if OxyHb induces apoptotic changes in cultured bovine aortic endothelial cells. METHODS: Confluent bovine aortic endothelial cells were treated with OxyHb in a concentration- and time-dependent manner. Cell density was assayed by counting the number of cells that attached to culture dishes after exposure to OxyHb. To identify apoptotic changes, the investigators used three specific methods: DNA fragmentation (electrophoreses), the apoptotic body (transmission electron microscopy), and cleavage of poly (adenosine diphosphate ribose) polymerase (PARP [Western blotting]). CONCLUSIONS: Oxyhemoglobin decreased cell density in a concentration- and time-dependent manner. Analysis of DNA showed a pattern of internucleosomal cleavage characteristic of apoptosis (DNA ladder). Transmission electron microscopy demonstrated condensation of nuclei and apoptotic bodies in OxyHb-treated endothelial cells. Western blotting with the PARP antibody revealed that the 116-kD PARP was cleaved to the 85-kD apoptosis-related fragment. These results for the first time demonstrated that the OxyHb induces apoptosis in cultured endothelial cells.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000近赤外分光法による内側広筋等尺性運動の酸素動態測定:評価の指標と信頼性に関する研究ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Double-Sector Lorenz Plot Scattering in an R-R Interval Analysis of Patients With Chronic Atrial FibrillationENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Comparison of Differential Diagnostic Capabilities of 201T1 Scintigraphy and Fine-Needle Aspiration of Thyroid NodulesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Anatomic relation between the medial collateral ligament of the elbow and the humero-ulnar joint axisENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000The Predictability of the Result of Secondary Osteoarthritis after Developmental Dislocation of the Hip, Utilizing Inferior Edge (of Teardrop) -Center (of Femoral Head) DistanceENFutoshiNagasawaWe followed-up a group of patients in their youth and then in middle age after they had been treated for developmental dislocation of the hip, and studied whether we could predict the progress of osteoarthritis of the hip when the patients were in the "youth" stage. We studied 21 hips of 21 patients with unilateral dislocation that could be examined twice, in 1975 and 1995, in patients who were treated at our hospital between 1953 and 1963. We measured the acetabular-head index (AHI), center-edge angle (CE angle), and the Sharp angle, and our created index (inferior edge of the teardrop - center of the femoral head distance), and we divided this index by the distance of the inferior edges of the teardrops on both sides. Using these measured values, we studied whether we could predict changes in clinical and radiographic evaluation after a follow-up of 20 years. There was no significant correlation of clinical and radiographic results and AHI, CE angle, and the Sharp angle; however, there was a significant correlation with our created index. The index we created is relatively easily measured and enables us to make a more precise prognosis, in comparison with previously developed indices.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000In Vitro Evaluation of Antimicrotubule Agents in Human Small-Cell Lung Cancer Cell LinesENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Combined Use of Cellulose Acetate Polymer and Retrievable Platinum Coils to Thrombose Experimental Cervical Carotid AneurysmsENNobuyukiHirotsuneNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2000Pergolide scavenges both hydroxyl and nitric oxide free radicals in vitro and inhibits lipid peroxidation in different regions of the rat brainENNo potential conflict of interest relevant to this article was reported.