Questionaires on subjective symptoms of fatigue and Donaffio reaction tests were conducted with fishermen and farmers. The results were as follows: 1. Of symptoms described on questionaires, eyestrain, wish to lie-down, loss of memory, and stiff-shoulder were higher in frequency among male fishermen, and stiff-shoulder was higher in frequency among female fishermen after work. Those having tired legs' wish to lie-down, and low back pain were of high frequency in both male and female farmers after work. Additionally in female farmers, stiff-shoulder was a frequent complaint. 2. Acute Donaggio values in urine showed a statistically significant increase in male fishermen. Actual Donaggio values and those values corrected for 1024 in urine showed statistically significant increases in female farmers.