Soluble factors of sensitized lymphoid cells that appear due to the difference in H-2 histocompatibility antigens Part 1. Study on the soluble factors by the tissue culture and diffusion chamber methods

For the in vitro studying transplantation immunity of mice the soluble factors of sensitized lymphoid cells were investigated by the tissue culture and diffusion chamber methods. As the result it was found that sensitized lymphoid cells obtained from A(H-2(a)) mouse sensitized 9 days previously by a skin graft from C3H (H-2(k)) mouse, and enclosed within the diffusion chamber did not inhibit the proliferation of the primary culture kidney cells from C3H mouse. However, sensitized lymphoid cells obtained from C3H mouse sensitized 9 days previously by a skin graft from A mouse, and enclosed within the diffusion chamber did inhibit the proliferation of the primary culture kidney cells from A mouse. In other words, the soluble factors elaborated by the sensitized lymphoid cells exert cytotoxic action on target cells, and such action seems to grow more marked as the difference in H-2 histocompatibility antigens becomes greater.