A study of estrogen binding protein in mammary tumor Part 1. An assay of estrogen binding protein

Estrogen binding protein (EBP) in mammary tumor was studied in DMBA induced rat mammary carcinoma, Shionogi carcinoma 115, Shionogi carcinoma 42 and human mammary tumor by use of (3)H-estradiol. Free and bound estradiol was separated by Sephadex G-25. Specific EBP was calculated 4.5S by sucrose gradient centrifugation. Non-specific EBP was observed in the 3.2S-3.6S region of the ultracentrifugal analysis. Specific EBP was migrated to anodic region of α- or β-position in disc electrophoresis.