Studies on relations of temperature of topically used cortico-steroid hormone (beta-methasone) to its therapeutic effect Part Ⅱ. Experimental studies on rabbit ear steel chamber technique

Using the rabbit ear steel chamber technique devised by Williams and modified by Ohsaki, biopharmacological reaction of topically applied cortico-steroid hormone was investigated. Solutions of cortico-steroid hormone (beta-methasone) kept under three thermal conditions, e.g., 37℃, 20℃, and 4℃, respectively, were poured into steel chambers with a microsyringe, and the changes of microcirculation of the vascularization in the chamber were observed chronologically. Changes of microcirculation were stasis, hemorrhage, sludge, and exsudate; and were set as the criteria of grade of inflammatory process. The results obtained were as follows: 1. Strong anti-inflammatory effects was showed by administration of beta-methasone at 37℃. 2. Beta-methasone at 20℃ showed less anti-inflammatory action than those at 37℃. 3. Application of beta-methasone at 4℃ acted as a vigorous inflammatory agent. This phenomenon may be attributed to the stimulating effect of cold solutions. Consequently, it seemed that topical application of beta-methasone was most effective when used at 37℃.