Studies on lymphadenopathy by tissue culture Part I. Application of clinical tissue culture and fluorochrominized tissue culture for the differential diagnosis of lymphadenopath

For the differentiation of the lymphadenomatous diseases the classical, histological methods have been generaly employed. Therefore, the clinical tissue culture and the fluorochrominized tissue culture both devised in our laboratory were applied for the differential diagnosis of the swollen lymph nodes, and the following results were obtained. 1) Findings in the clinical tissue culture of lymph nodes.: The growth zone of the tissue culture of non-pathologic lymph node was densely packed with cells in the central portion, but less densely in the peripheral portion. In the lymph node of lymphadenits simplex, the cell growth was more active than that of non-pathologic one, while in the lymph node of reticulosarcoma, tuberculosis, Hodgkin's disease and metastasis of cancer, the cell growth was mostly poor. 2) Fluorescence microscopic findings of the fluorochrominized tissue culture of lymph nodes.: The growth zone of non-pathologic lymph node presented diffusely yellowish green color, sparsely intermingled with reddish orange fluorescent spots. As to the pathologic lymph node with poor cell growth, tissue culture in both reticulosarcoma and Hodgkin's disease presented the same yellowish green color with reddish orange fluorescent spots, but in Hodgkin's disease fluorescence had more reddish hue. In metastatic lymph nodes of cancer, massive reddish orange fluorescence within the green explant was observed because of cancer tissues. 3) To conclude these results, the differential diagnosis of the swelling of lymph nodes could be facilitated by the clinical and fluorochrominized tissue culture of lymph nodes.

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