Electronmicroscopic Studies on Supravitally-Stained Granules Part 2. Electronmicroscopic Studies on Supravital Staining of Golgi Apparatus by Neutral Red

After fixing the bone marrow cells supravitally stained with neutral red by the method, the auther's own device as mentioned in Part 1, the author succeeded in detecting supravitally stained neutral red granules under the electronmicroscope. As the result, it has been clarified that neutral red does not stain Golgi apparatus specifically as generally believed nor does it stain specifically any other organellae in the cell. Namely, the dye is found within cytoplasma only in the form of dye droplets independent of organellae. It is assumed that the tendency of this dye-droplets gathering especially in the region of Golgi apparatus is due to the mechanism which attracts all the foreign substances invaiding the cytoplasma in that region or to a purely physicochemical phenomenon, the gathering at the boundering of two different colloid phases.