Experimental Studies on Aspirin Allergy Part 3. Localized Skin Allergy induced by Aspirin-protein

There are already many reports in various allergic reactions by aspirin, but most of them deal with the reactions induced by aspirin alone or a physical compound of aspirin and serum protein, as the antigens. For this reason they failed to demonstrate the antigen-antibody reaction in vitro although they recognized the establishment of active allergy. In addition, there are few reports in which conjugates of aspirin to serum proteins are used as antigen, but there seems to be no report on any experimental localized passive allergy. In the present experiment, the author selectively sensitized rabbits and guinea-pigs using conjugates of aspirin to proteins or aspirin solutions as the antigens, and studied the localized skin allergy in these test animals. The results are as follows. 1. In the rabbits and guinea-pigs sensitized with aspirin-protein, active and passive Arthus' reactions have been recognized. 2. In the above-mentioned active and passive Arthus' reactions, a cross reaction has been recognized to occur by heterogous antigen just as in the case of the precipitation reaction. 3. In the sensitization of guinea-pigs using aspirin alone, it has been recognized that there occurs the active Arthus' reaction by aspirin-proteins and by aspirin. 4. In the active Arthus' reaction induced by the aspirin-protein, there can be recognized some reaction differences between the sensitizing antigen and the cross-antigen. The grade of these reactions is more marked in the former. 5. In the active Arthus' reaction induced in the guinea-pigs sensitized with aspirin alone, no reaction differences such as mentiones above can be recognized. 6. In the passive Arthus' reaction there can't be recognized some reaction differences between the corresponding antigen and the chemical cross antigen. 7. The animals sensitized either actively or passively with aspirin have been found to respond to sodium salicylate.