Further Studies on Distribution of P(32) Labeled Salmonella in Mouse

In order to ascertain the distribution of bacteria introduced into an animal body, the author carried out the further studies on this subject. The movement of bacteria into liver, spleen, kidney and lung was traced by observing the radioactivity of P(32) up to 60 hrs. P(32) labeled Salmonella 57 S were obtained by culturing the organism in bouillon media for 18 hrs to which P(32) was added, and were injected into body of mouse through various pathways. The following results were obtained. 1) The rate of reduction of P(32) in various organs was slower in the case of P(32) labeled Sal. 57 S than in the case of direct injection of P(32) into body. The amount of P(32) in the spleen was found to be specially high. 2) The rate of reduction of P(32) was much slower in the mice that were previously immunized against organisms. The reduction of radioactivity on the various organs was not observed 30 hrs after injection. The radioactivity on organs were found to be increased in the case of subcutaneous injection after 30 hrs as above. The concentration of P(32) was especially high in spleen when the organisms were injected intraperitoneally.