Studies on the Oxygen Consumption and Glycolysis of Bone Marrow Part 3. The Effects of Antileukemic Agents on the Oxygen Consumption of Rabbit Bone Marrow

With Warburg's apparatus the author studied the effects of the addition of various antileukemic agents such as nitromin, myleran, 8-azaguanine, 6-mercapurine, prednisolone, ACTH, actinomycin C, and carzinophilin directly to the bone marrow of the femur of normal rabbits, and obtained the following results. 1. Nitromin at the concentration of 10(-1)g/dl seems to inhibit the respiration of the bone marrow, and the oxygen consumption every 10 minutes decreases along with laps of the time. 2. Myleran has no effect on the respiration of the bone marrow. 3. 8-azaguanine has likewise no effect on thc respiration of the bone marrow. 4. 6-mereaptopurine at the concentrations of 5×10(-1)g/dl and 5×10(-2)g/dl seems to inhibit the respiration of the bone marrow. 5. Prednisolone at the concentrations of 2×10(-1)g/dl and 2×10(-2)g/dl seems to inhibit the respiration of the bone marrow. 6. ACTH at the concentrations of 2×10(-2)unit/dl, 2×10unit/dl and 2unit/dl seems to accelerate the respiration of the bone marrow. 7. Actinomycin C at the concentration of 2×10(-5)g/dl accelerates the respiration of the bone marrow slightly, but in any other concentration it does not differ from that of the control, suggesting that it does not inhibit the respiration of the bone marrow, 8. Carzinophilin hardly inhibits the respiration of the bone marrow, but at the concentration of 5×10(-4)unit/dl every ten minutes the oxygen consumption somewhat tends to decrease along with lapse of the time. From these it is assumed that nitromin, 6-mereaptopurine and prednisolone at a specific concentration act directly on the bone marrow and inhibit its respiration, but ACTH directly accelerates the respiration of the bone marrow and thus enhances the hematopoietic fucntion.