With the purpose to see whether or not anamnetic reaction is induced by hormones of the pituitary and adrenal glands and to study its clinical significance, after disappersance of the homologous-liver-antibody and auto-liver-antibody that have been once produced experimentally in animals, the author performed a series of experiments with animals and obtained the following results. 1. Of pituitary and adrenal hormones ACE markedly elicits anamnetic reaction, homologous and at-liver-antibodies, and moreover, it has becoome clear that these antibodies make their appearance in the circulating blood within one to five days of the injection of these hormones. 2. Clinically this fact increases the imporsance of observing the auto-liver-antibody. 3. The use of an appropriate dose of nitrcmin can prevent this anamnetic reaction form occurring.