Cytological Studies on the Explanted Bone Marrow Tissue by Stained Section Specimens Part 2. Bone Marrow Tissue Culture of the Normal, the Myelogenous Leukemic, and the Aplastic Anemic Patients

In the observation on the tissue culture of bone marrow from normal persons, myelogenous leukemic and aplastic anemic patients the following conclusions were arrived at: 1. Like in the case of bone marrow tissue culture of rabbits, in the bone marrow tissue culture of normal persons wandering cells wander out from the explanted bone marrow, thus forming a growth zone; and at the same time cells without wandering capacity make their appearance in the center of the growth zone. In culture immature cells mature, and having the peak of mitotic index at the 12-hour culture, it rises and falls. In the explant of bone marrow after 4 to 5 days interstitial cells proliferate and the bone marrow tissue loses its peculiarity. Namely, even in the bone marrow tissue culture of the human, the bone marrow cell functions of maturation, multiplication and migration have been recognized again. 2. In the bone marrow tissue culture of myelogenous leukemia the cell maturation in the explanted bone marrow is delayed, and in that of acute form even cultured for a long period of time immature cells can not mature completely and they degenerate in the explanted bone marrow. Although the cell density is high and many immature cells appear in the growth zone, the boundary of growth zone in acute form is clear-cut and in chronic form many matured cells can be observed around the boundary of growth zone. The mitotic index in clture in chronic form maintains a higher value than that in normal person, but in acute form it falls along with the lapse of time. 3. In the tissue culture of aplastic anemia both maturation and mitosis are obstructed, and an increase and enlargement of fat cells, and an increase in mast cells can be recognized.