Experimental Studies on the Effect of Photosensitizing Dye, Platonin, upon the Antibody Productions Chapter One Experimental Studies on the Changes That Occur Either in the Reticuloendotherial System, Serum Protein, or the Antibody Production of Immunized Rabbits

After having once injected cow's serum antigen toward both a normal rabbit, as well as a rabbit with impediments in the reticuloendotherial function due to ink injection, I examined and have taken observations on such matters as Congored Index, total serum protein density, precipitin value, as well as quantitative changes that happened; which, offered results as follows: 1. In case of a normal one, the total protein density of antiserum, along with γ-Globulin quantity, almost showed a parallel relation with the precipitin changes. In those viccisitudes that have occurred in α- and β-Globulin as well as Congorccl Index, no definite tendency could be detected. 2. In case of a splenorectomized rabbit, there occurred an increase in Congored Index, accompanied with a decrease in the total serum protein density, albumin, as well as γ-Globulin, on the day that followed the operation. Moreover, an increase has taken place in β-Globulin. 3. In an ink-filled rabbit, there could be seen, within a day of injection, certain increase in the Congored Index, along with a decrease in total serum protein density, albumin or γ-Globulin. 4. In those rabbits who underwent splenorectomy and ink-injection, no serum protein acting parallel with the precipitin changes, as seen in a normal one, could be observed. 5. The precipitin production has proved at its maximum in a normal rabbit, in 20 days after antigen injection; while, in 20-30 days in case of an operated one; and 14 days in the injected rabbit, among these three groups, the injected rabbit showed maximum; next, normal one, and lastly, rabbit who suffered the operation. 6. At 85 th days after the injection, of antigen, a decline in precipitin has proved marked in all the three groups, esp., very eloquent in an operated rabbit. 7. From the above results, it may roughly be concluded that a close correlation exists between γ-Globulin and antibody; further, the role of reticuloendotherial system as mother ground for antibody production may be considered with some significance.