Biological Reaction to a Dye Protein II. Distribution of injected Congo red-azo-dye-protein in tissues

1) Introducing dye protein (Congo red-oxen serum, DPO No.3) into the normal adult guinea pigs from three roots, subcutaneous tissue, intraperitoneal cavity and blood vessel the distribution of dye protein in the tissues and organs has been traced histollogically. 2) The distribution of dye protein in the tissues varied according to the varied roots of introduction. The dye protein were found in the granulocytes and macophages in the early stage of injection but in the later stages only in macrophages, those in the local tissues and in the sinuses of the regional lymphnodos in the case of subutaneous injection, those mainly in the omentum in the case of intraperitoneal injection, and those belonging to the reticuloendotherial system mainly of liver and spleen in the case of intravenous injection. 3) The protein uptake of granulocytes and macrophges occurred independently from each other showing no intimate correlation between them in the sense of Sabin who asserted that the protein uptake of macrophges is caused by the phagocytosis of the protein containing granulocytes. 4) Concerning the period of the reservation of protein in macrophages the water soluble dye protein disappeared from the cells already 40 days after the injection, though the insoluble dye protein which had been prepared treating with alcohol proved to be remained in the cells so long as 90 days after the injection.