Studies on the Specifity in Direct Functions of Leukocyte Autolysate. Ⅲ. Additional Tests towards the Tissue Cultures of the Bone-marrow and Lymphnodes, and Conclusionsto Entire Series

In the previous chapters, I have described the so-called direct functions indicated by pseudoeosinocytes, eosinophil leukocytes, as well as lymphocytes, at the same time examining their mechanism. In this, I am going to discuss the case in which the above mentioned leukocytic extract has been added to the tissue culture of the bone-marrow and lymphnodes. 1) In case pseudoeosinocyte extract are added to any tissue culture of rabbit's bone-marrow, an improvement of tissue growth rate (i.e., an increase in pseudoeosinocytes) as well as an excitement in their wandering velocity are achieved in comparative particularity. 2) If I add lymphocytic extract to a rabbit's lymphnode culture, there happens in comparative unique mode, an improvement in the tissue growth rate (i.e., lymphocytic increase), and the lymphocyte rises in its wandering velocity. 3) If I add guinea-pig's eosinophil leukocytes extract on the culture of the same animal's　bone-marrow, it induce an increase of eosinophil leukocytes in the growth zone, together with a rise in the wandering velocity of the same. 4) I evidenced that the direct influence of leukocyte extract explained in 1st and 2nd chapter, acts on the bone-marrow and lymphnodes in a direct manner. Conclusions. Owing to those ezperimental results obtained throughout these chapters, I can conclude as folllows: i.e. The extracts stimulate the bone-marrow or lymphnode directly with comparative specifity, no matter how the liver and spleen might stand, and induce the increase of each sort of leukocytes respectively and of their. wandering velocity so that they appear in the peripheral blood in short time, though it is better that the reticuloendothelial system is normal.