Effects of X-iradiation on Amylase Action

In order to clarify what change might occur to starch saccharification in case 80, 200, or 400r X-Ray has been applied immediately after mixing of starch, taka-diastase and buffer-solution, we have conducted experiment, employing 5.0 and 20.0 cc respectively of 2.0, 1.0 as well as 0.5% starch solution, together with 1.0 and 4.0 cc each of 1.0, 0.5 and 0.1% taka-diastase solution, and obtained the following conclusions; 1) The starch saccharification of taka-diastase is to be either activated or inhibited due to the doses of the applied X-Rays, i.e., activated at 80r iradiation, while, inhibited at 200 and 400r. 2) In case auy starch saccharification due to taka-diastase should reach its limit the quantity of glucose in saccharification solution indicates none of effects shown in cases when 80, 200, and 400r have been iradiated. 3) The effect of X-Ray on starch saccharification of taka-diastase appears rather poignantly in areas of low-concentration (from 0.1 to 1.0%). Also, if we compare 80r (activated case) with 200 & 400r (cases of inhibition), the cases of activation seem to have been influenced more strongly than in cases of inhibition. 4) When X-Ray has been iradiated on the mixture of taka-diastase, starch and buffer solution, the percentage both of decrease as well as increase that has happened to the supar quantity within 3 hours after the iradiation, proves greater than that observed 6 hours later. 5) If the concentration of starch and taka-diastase solutions proves equal, in case these quantities have been augmented 4 times, almost no difference whatever should occur to the chnges of sugar quantity percentage.