Correlation between Amylase Action and Concentration of Starch as well as of Taka-Diastase solution

In the saccharification of starch by taka-diastase, in order to clarify to certain extent the correlation that exists between the concentration for both and their final decomposition product, i.e., the quantity of glucose, we have carried out several experiments; the results of which are as follows: (1) Saccharification of starch (2.0, 1.0 and 0.5% solution) by taka-diastase (1.0, 0.5 and 0.1% solution) is brought about very rapidly at the earlier stage of reaction; however, it relaxes rather rapidly, reaching its limit in a certain time. (2) In saccharification of starch, after it has reached its limit, the glucose quantity does not manifest any increase, even if we augment the concentration of taka-diastase solution (ranging from 0.1 to 1.0%), or lengthen the incubation time (to the extent of 72 hours). (3) In case the concentration of starch solution proves fixed (in 0.5, 1.0 or 2.0%), (a) the greater the concentration of taka-diastase solution is, (as to range from 0.1 to 1.0%), the rapider in its saccharification; besides, time needed in order to reach limit, short, and, glucose amount at a certain time (1/2, 3, 6, 12 and 24 hours) proves abundant, but not correspond to the concentration of enzyme solution. (b) When concentration of takadiastase solution is 1.0 and 0.5%, glucose quantity for both at the limit of saccharification proves equal, which has been estimated as about 80% or more of the amount of the final product of starch hydrolysis (by Liebermann's method); moreover, nearly corresponds to concentration of enzyme solution. (c) If the concentration for taka-diastase solution indicates 0.1%, the glucose quantity at the limit of saccharification is to prove approximately 70% or more of the amount of the final product of starch hydrolysis, proving less compared to that shown in 1.0 and 0.5% cases of enzyme solution. (4) When the concentration of taka-diastase solution remains fixed (in 0.1, 0.5 or 1.0%), (a) as the concentration (that ranges from 0.5 to 2.0%) of starch solution proves higher, time needed for it to reach saccharification limit becomes longer. (b) In case the concentration of taka-diastase solution indicates to be 1.0 and 0.5%, glucose amount at the limit of saccharification of 1.0% starch solution proves twice as much; while, that of 2.0% starch solution, compared to that of 0.5% starch solution, proves four times as much. (c) In case the concentration of taka-diastase solution is 0.1%, it is not enough for its saccharification, if starch solution proves 2.0%; while glucose quantity at the limit of saccharification of 1.0% starch solution indicates twice as much of that of 0.5% starch solution, that of 2.0% starch solution does not prove 4 times as much as that of 0.5% starch solution.