THE CLINICAL AND EXPERIMENTAL STUDY ON THE COURSE OF SYPHILIS WITH PENICILLIN PART III THE INFLUENCE OF PENICILLIN ON THE COURSE OF PRECIPITATION (MURATA AND KAHN) IN EXPERIMENTAL RABBIT SYPHILIS

I have injected penicillin in rabbits in which spirochaeta pallida was oculated at the period when the precipitation (Murata and Kahn) turned positive and observed the transition of the serum reaction, alteration of the local state and disappearance of spirochaeta from the local area and obtained the following results: 1) In the cases in which 50,000 U. of penicillin were injected, the influence of penicillin on the 3 reactions (WaR, Murata and Kahn) was rather unstable and the term needed for the 3 reactions to turn negative was prolonged, i.e., WaR turned negative in 66.5 days after the oculation (54.5 days after the injection), Murata's reaction in 94.5 days (82.5 days after the injection), and Kahn's reaction in 91.0 days (79.0 days after the injection). 2) In the 100,000 U. injected cases, WaR turned negative in 63.0 days after the oculation (50.7 days after the injection), Murata's reaction in 84.0 days (71.7 days after injection), Kahn's reaction in 92.8 days (80.5 days after injection). 3) In the cases in which 200,000 U. were injected, a definite effect of penicillin on the serum reaction was observed when compared with the previous 2 groups, i.e., WaR turned negative in 11.6 days after oculation (7.0 days after injection), Murata's reaction in 28.0 days (18.6 days after injection), and Kahn's reaction in 46.6 days (37.3 days after injection). 4) In the observation of the effect of penicillin on the local state: in 50,000 U. injected cased, stuntedness of the local state and the prolongation of the incubation could be observed and the local state cured in 36.0 days, in 100,000 U. injected cases, the prolongation of incubation could be observed but the local state occured and took 25.7 days to disappear and in 200,000 U. injected cases, the local state was completely prevented and the syphilitic local symptoms did not appear. 5) 200,000 U. of penicillin showed a definite effect on the existence of spirochaeta, i.e., the spirochaeta conld not be detected at all, and in 50,000 U. injected cases, the effect of injection was not distinct and they disappeared in 16.0 days in the former cases and in 9.2 days in the latter cases. 6) The recurrens of the serum reaction and the local state could not be observed during the observation of 4 months on the average. Comparing the above results with those described in Part II, where the injections were given at the period when WaR turned positive, the following results were obtained: 7) Concerning the 50,000 U. and 100,000 U. injected cases, the term needed for the serum reaction to turn negative was prolonged in the cases in which the injections were given at the period when precipitation turned positive, when compared with the cases in which injections were given at the period when WaR turned positive, therforee the time required for the absorption of local state and the disappearance of spirochaeta was also prolonged. 8) In the observation of 200,000 U. injected cases: WaR turned negative in 42.6 days after oculation (21.3 days after inlection), Murata in 56 days (34.6 days after injection), Kahn in 60.6 days (39.3 days after injection) in the cases in which injections were given at the period when WaR turned positive, and in the cases where injections were made at the period when precipitation turned positive, WaR turned negative in 11.6 days after oculation (7 days after injection), Murata in 28.0 days (18.6 days after injection), Kahn in 46.6 days (37.3 days after injection), i.e., distinct reduction could be observed in the latter cases. 9) In 200,000 U. injected cases, a remarkable and regular effect of penicillin could be observed when compared with other 2 groups, and especialy in the cases in which injections were given at the period when the precipitation turned positive the effect was more distinct. 10) In 50,000 U. and 100,000 U. injected cases no distinct and regular effect of penicillin could be observed.