検索結果 105 件
| タイトル（別表記） | Annual report 2009 / Research Institute for Bioresources Okayama University |
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| フルテキストURL | 017.pdf |
| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2010-03-31 |
| 巻 | 17巻 |
| 開始ページ | 1 |
| 終了ページ | 45 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Annual Report 2008 / Research Institute for Bioresources, Okayama University |
|---|---|
| フルテキストURL | 016.pdf |
| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2009-03-31 |
| 巻 | 16巻 |
| 開始ページ | 1 |
| 終了ページ | 47 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Annual Report 2007 / Research Institute for Bioresources, Okayama University |
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| フルテキストURL | 015.pdf |
| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2008-03-31 |
| 巻 | 15巻 |
| 開始ページ | 1 |
| 終了ページ | 43 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Annual Report 2006 / Research Institute for Bioresources, Okayama University |
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| フルテキストURL | 014.pdf |
| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2007-03-31 |
| 巻 | 14巻 |
| 開始ページ | 1 |
| 終了ページ | 50 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | 013.pdf |
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| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2005 |
| 巻 | 13巻 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | 012.pdf |
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| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2004 |
| 巻 | 12巻 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | 011.pdf |
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| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2003 |
| 巻 | 11巻 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | 010.pdf |
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| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2002 |
| 巻 | 10巻 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | 009.pdf |
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| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2001 |
| 巻 | 9巻 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | 008.pdf |
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| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 2000 |
| 巻 | 8巻 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | 007.pdf |
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| 著者 | 岡山大学資源生物科学研究所| |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1999 |
| 巻 | 7巻 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Mutation of Fungicide Tolerance in Fusarium spp. |
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| フルテキストURL | 005_001_047_060.pdf |
| 著者 | 呉 基日| 武田 和義| |
| 抄録 | Strains of Fusarium, causal fungi of scab disease of cereal crops, were examined for the mutation of topsin tolerance. The mutation rate of topsin tolerant spores was 10-5~10-6 and was not affected by the irradiation of ultraviolet rays nor application of topsin to the culture medium. Highly tolerant strains were easily selected on the screening medium containing topsin. Finally topsin was not effective to the selected tolerant mutants. The topsin tolerance was parallel to "benrate" tolerance. The tolerance was transmitted through hyphae, conidium and ascospore. The growth rate of hyphae of the tolerant mutants was lower than that of the original strains, but the conidia formation and the virulence of the mutants were comparable to the original strains. Since the heavy application of fungicides may increase the fungicide-tolerant mutants, crop varieties resistant to scab disease must be developed. |
| キーワード | Fusarium spp. Fungicide tolerance Mutation. |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 47 |
| 終了ページ | 60 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Phenol Reaction of Kernels and Chromosome Location of Phenol Reaction Genes in the Genus Triticum |
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| フルテキストURL | 005_001_061_068.pdf |
| 著者 | 張 成林| 武田 真| 武田 和義| |
| 抄録 | A total of 3,606 accessions of the genus Triticum involving diploid, tetraploid, hexaploid, and synthetic wheat and 7 Aegilops materials were tested for the phenol reaction in kernels. All hexaploid wheat showed a positive reaction to phenol, but deffered in staining degree. One of the synthetic wheat lines showed a negative reaction to phenol. Using monosomics, ditelosomics and nulli-tetrasomics in the common wheat cv Chinese Spring (Triticum aestivum L.), and Langdon (Triticum turgidum var. durum) disomic substitutions, genes controlling phenol reaction of kernels were located on chromosomes 2A, 2B and 2D. Synteny of the chromosome region involving the phenol reaction gene in some gramineous plants was discussed. |
| キーワード | Phenol reaction Triticum Chromosome |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 61 |
| 終了ページ | 68 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ラン科植物に発生するシンビジウムモザイクウイルスの血清学的検出 |
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| フルテキストURL | 005__001_039_046.pdf |
| 著者 | イ ワヤン ガラ| 近藤 秀樹| 前田 孚憲| |
| 抄録 | Dot-immunobinding assay (DIA) on nitrocellulose membranes and rapid immunofilter paper assay (RIPA) were examind for their usefulness in the detection of cymbidium mosaic virus (CyMV) in orchids. The minimum detection levels of CyMV by these methods were 100 ng/ml in purified preparations and at 10-4 dilution of extracts from infected leaves of orchids could be detected by these methods. Although DIA took 5 to 6 hours for the detection of the virus, it was reliable method for diagnosis of a large-number of samples. On the other hand, RIPA, which enabled detection of CyMV within a few minutes with sensitivity similar to that of DIA, will be suitable as a rapid and handy tool for virus disease diagnosis in orchids. Moreover, by RIPA, we could detect CyMV and odontoglossum ringspot virus (ORSV) simultaneously form doubly infected plant. |
| キーワード | Serological detection Cymbidium mosaic virus Orchid |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 39 |
| 終了ページ | 46 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313874 |
| タイトル（別表記） | QTL Mapping for Powdery Mildew (Erysiphe graminis DC. f. sp. hordei EM Marchal) Resistance in Barley (Hordeum vulgare L.) |
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| フルテキストURL | 005_001_069_078.pdf |
| 著者 | 岩佐 友彦| 部田 英雄| 武田 和義| |
| 抄録 | Powdery mildew, caused by Erysiphe graminis DC. f. sp. hordei EM Marchal, is a serious disease of Barley (Hordeum vulgare L.). In this study, we used molecular markers to identify the chromosomal locations carrying genes for powdery mildew resistance and to estimate the effect of each gene. Doubled haploid lines derived from Steptoe×Morex (S/M), Harrington×TR306 (H/T) and their parental were inoculated with five powdery mildew strains. Several quantitative trait loci (QTL) controlling E. graminis resistance were found and lacated on chromosome 4H, 5H, 6H and 7H in S/M. On the other hand, no QTL was detected in H/T but Harrington had a major resistant gene (Mlg) for powdery mildew resistance. Maker-assisted selection was conducted to examine the effect of accumulation for mildew resistance. There was a significant interaction between QTLs lacated in 4H and 7H, which suggested the presence of an epistatic effect between these QTLs. |
| キーワード | Hordeum vulgare Erysiphe graminis QTL mapping DH lines |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 69 |
| 終了ページ | 78 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 日本におけるCymbidium属植物から分離されて生物学的性質の異なるOdontoglossum Ringspot Virus分離株のペプチドマッピングによる比較 |
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| フルテキストURL | 005_001_031_038.pdf |
| 著者 | 近藤 秀樹| 前田 孚憲| 井上 成信| |
| 抄録 | Symptoms on Cymbidium, double-stranded (ds) RNA pattern and peptide mapping of coat protein (CP) of five isolates of odontoglossum ringspot virus from Cymbidium in Japan were compared. One of the isolates, Cy-1, that produced unique symptoms on Cymbidium, showed a distinct peptide mapping pattern from those of the other four isolates by partial digestion of CP with pepsin. All the isolates produced three major dsRNA species of Mr=4.3,1.4 and 0.6×106 in the infected plants. |
| キーワード | Odontoglossum ringspot virus Cymbidium Peptide mapping Double-stranded RNA |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 31 |
| 終了ページ | 38 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313419 |
| タイトル（別表記） | 2種のモノクローナル抗体を用いた簡易ELISAによるキュウリモザイクウイルス迅速・高感度検出 |
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| フルテキストURL | 005_001_023_030.pdf |
| 著者 | 前田 孚憲| 佐古 宣道| 井上 成信| |
| 抄録 | To detect cucumber mosaic virus (CMV), virus samples and conjugate were incubated together in a simplified double-antibody sandwich ELISA. The use of the same monoclonal antibody (MAb) as trapping (coating) and detecting antibodies resulted in considerable decrease of ELISA values and sensitivity due to the competition for antigen between trapping and detecting antibodies. The simplified ELISA using two MAbs which recognize different epitopes of CMV proved to be a rapid and sensitive method for virus detection. |
| キーワード | Cucumber mosaic virus Monoclonal antibody Simplified ELISA |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 23 |
| 終了ページ | 30 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ジゴキシゲニン標識プローブを用いたBeet Necrotic Yellow Vein Virus RNA の検出 |
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| フルテキストURL | 005_001_079_096.pdf |
| 著者 | 齊藤 美奈子| 木口 忠彦| 玉田 哲男| |
| 抄録 | Complementary DNA (cDNA) clones corresponding to each of five distinct RNA species of beet necrotic yellow vein (BNYVV) were synthesized and identified. The sizes of each cDNA clone for RNAs 1,2,3,4 and 5 molecules were 3.0, 1.7, 1.8, 1.5 and 1.4 kbp, respectively. cDNA inserts to RNA 2 were covered at a part of the 3'regions, and those of RNAs 3,4 and 5 were almost full-length. The plasmids containing each of cDNA inserts were labeled with digoxigenin by the random priming method. Northern blot hybridization tests showed that individual probes hybridized specially to each of the five RNAs. Good results were obtained with 1 to 10 ng of RNA as a mixture of five RANs, but the probe to RNA 3, RNA 4 or RNA 5 gave a weak signal with hererologous RNAs when more than 10 ng RNA was used. In dot blot hybridization, the limit of detection was about 10 pg RNA, but if a higher content of RNA was spotted, cross reaction occurred using heterologous RNAs. For laboratory and field isolates of BNYVV, each of RNAs 3,4 and 5 was easily detected by Northern blot hybridization in total nucleic acids extracted from Tetragonia expansa leaves inoculated mechanically, but not from roots of sugar-beet plants inoculated by the fungus Polymyxa betae. However, satisfactory results were obtained with partially purified or concentrated preparations from roots. These findings indicate that the digoxigenin-labeled probes are useful for the identification and detection of RNAs contained in field and laboratory isolates of BNYVV. |
| キーワード | Sugar beet Rhizomania BNYVV RNA detection Nonradioactive cDNA probe |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 79 |
| 終了ページ | 96 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Measurements of Response of Barley and Wheat to Enviromental Factors with an Open System Porometer |
|---|---|
| フルテキストURL | 005_001_011_021.pdf |
| 著者 | 米谷 俊彦| 柏木 良明| |
| 抄録 | The rates of photosynthesis and transpiration were measured in barley and wheat under various environmental conditions, with an open system porometer. The rates of photosynthesis and transpiration in the horizontal leaf and vertical leaf had different diurnal variations. The rate of photosynthesis in the vertical leaf was highest in the morning and in the afternoon, while that in the horizontal leaf was highest before noon. The rates of photosynthesis and transpiration and chlorophyll contents were measured for two species(c.v SARI and Akanmugi) of barley grown in submerged soil conditions. At the end of April, chlorophyll contents had decreased and the maintenance respiration acquired in spite of continuous transpiration. The rapid change of photosynthetically active radiation did not affect the rates of photosynthesis or stomatal conductance of SARI grown in submerged soil. The rates of photosynthesis and transpiration and chlorophyll contents were measured for two species(c.v. Hongmangmai and Haruhikari) of wheat grown under different soil water conditions. Chlorophyll content tended to increase in dry soil conditions. Hongmangmai had a higher chlorophyll content than Haruhikari, even at the beginning of May. Hongmangmai had large photosynthetic rate and small transpiration rates under dry soil conditions. These confirm that Hongmangmai has a prominent drought stress tolerance. The open system porometer and the chlorophyll meter may be very useful for comparing physiological characteristics of the plant's response to environmental factors and clarifying differences between plant species. |
| キーワード | Barley Hongmangmai Photosynthesis rate Chlorophyll content Submerged soil Dry soil |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 11 |
| 終了ページ | 21 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ライムギ小型染色体を保持する普通系コムギからのライムギ型cDNAのディファレンシャルスクリーニング |
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| フルテキストURL | 006_001_053_064.pdf |
| 著者 | 村田 稔| |
| 抄録 | Occurrence of the midget chromosome in a common wheat with rye cytoplasm [(cereale)-Chinese Spring (CS)] indicates that the chromosome carries the essential gene(s) for maintaining the function of rye cytoplasm. To elucidate the interaction between the midget chromosome and rye cytoplasm, in this study, an attempt was made to isolate rye-type cDNAs from a cDNA library of (cereale)-CS by differential screening. Two replica filters from each plate were hybridized with digoxigenin (DIG)-labeled wheat CS cDNAs and with DIG-labeled rye cDNAs,respectively. Out of ca. 20,000 plaques, 27 were hybridized more strongly with rye cDNAs than with CS cDNAs. These clones were classified into six classes (Ⅰ-Ⅵ) by blot hybridization. The majority of the clones (21 out of 27) was belonged to the same class (1), showing rye-type RFLP (restriction fragment length polymorphism). The DNA sequence of clone CrClA in class Ⅰ, was very similar to that of wheat ribulose 1,5-bisphosphate carboxylase,large subnit gene, rbcL(94.5% homology). However, the 3' end of CrClA was shorter than that of wheat rbcL, and terminated at TAA instead of TAG, like the rbcL of Aegilops crassa. In the clone CrC5.4, the first half of the sequence was similar to that of one rice EST clone, the functions of which are not known, and the latter was similar to the reverse sequence of maize 4.5S-23S ribosomal RNA. This suggests that CrC5.4 had been derived from two defferent cDNAs of (cereale)-CS. Three other clones had homology to the chlorophyll a/b binding protein genes (cab) of wheat, maize and tomato, and one to wheat rbcS (ribulose1,5-bisphosphate carboxylase small subnit gene). However, no clear polymorphisms were detected between wheat and rye by using those clones as probes. |
| キーワード | Cytoplasm substitution line Differential screening Midget chromosome Rye Wheat |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1999 |
| 巻 | 6巻 |
| 号 | 1号 |
| 開始ページ | 53 |
| 終了ページ | 64 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |