このエントリーをはてなブックマークに追加
ID 52034
フルテキストURL
著者
Liu, Ning Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Shang, Jingwei Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Tian, Fengfeng Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
Nishi, Hiroyoshi Yokohama Coll Pharm, Dept Clin Pharmacol
Abe, Koji Okayama Univ, Dept Neurol, Grad Sch Med Dent & Pharmaceut Sci
抄録
Detection and protection of apoptosis, autophagy and neurovascular unit (NVU) are essentially important in understanding and treatment for ischemic stroke patients. In this study, we have conducted an in vivo optical imaging for detecting apoptosis and activation of matrix metalloproteinases (MMPs), then evaluated the protective effect of 2 package types of free radical scavenger edaravone (A and B) on apoptosis, autophagy and NVU in mice after transient middle cerebral artery occlusion (tMCAO). As compared to vehicle treatment, edaravones A and B showed a significant improvement of clinical scores and infarct size at 48 h after 90 min of tMCAO with great reductions of in vivo fluorescent signal for MMPs and early apoptotic annexin V activations. Ex vivo imaging of MMPSense 680 or annexin V-Cy5.5 showed a fluorescent signal, while which was remarkably different between vehicle and edaravone groups, and colocalized with antibody for MMP-9 or annexin V. Edaravone A and B ameliorated the apoptotic neuronal cell death in immunohistochemistry, and activations of MMP-9 and aquaporin 4 with reducing autophagic activations of microtubule-associated protein 1 light chain 3 (LC3) in Western blot. In this study, edaravone in both packages showed a similar strong neuroprotection after cerebral ischemia, which was confirmed with in vivo and ex vivo optical imagings for MMPs and annexin V as well as reducing cerebral infarct, inhibiting apoptotic/autophagic mechanisms, and protecting a part of neurovascular unit.
キーワード
Apoptosis
Autophagy
Cerebral ischemia
Edaravone
In vivo imaging
発行日
2011-06-23
出版物タイトル
Brain Research
1397巻
出版者
Elsevier Science Bv
開始ページ
66
終了ページ
75
ISSN
0006-8993
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1016/j.brainres.2011.04.038
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/51965
言語
English
OAI-PMH Set
岡山大学
著作権者
(C) 2011 Elsevier B.V. All rights reserved.
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT