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ID 56688
フルテキストURL
著者
Elgaml, Abdelaziz Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayama University
Higaki, Kazutaka Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayama University
Miyoshi, Shin-ichi Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayama University
抄録
Vibrio vulnificus is a halophilic estuarine bacterium while it causes fatal septicemia or necrotizing wound infections in humans. This pathogen secretes the metalloprotease (V. vulnificus protease: VVP) and the cytolysin (V. vulnificus hemolysin: VVH) as protein toxins; however, their production was coordinated in response to the bacterial cell density. This regulation is termed quorum sensing (QS) and is mediated by the small diffusible molecule called autoinducer 2 (AI-2). In the present study, we investigated effects of disruption of luxO encoding a central response regulator of the QS circuit, as well as effects of temperature and growth phase, on the toxin production by V. vulnificus. Disruption of luxO was found to increase VVP production and expression of its gene vvpE. The expression of smcR, crp and rpoS, of which products positively regulate vvpE expression, and luxS encoding the AI-2 synthetase were also significantly increased. On the other hand, the luxO disruption resulted in reduction of VVH production and expression of its gene vvhA. Expression of other two genes affecting the QS circuit, luxT and rpoN, were also significantly decreased. The regulation systems of VVP production were found to exert their action during the stationary phase of the bacterial growth and to be operated strongly at 26 °C. By contrast, those of VVH production apparently started at the log phase and were operated more effectively at 37 °C.
キーワード
Vibrio vulnificus
Metalloprotease
Hemolysin
Quorum sensing
Autoinducer
備考
This is an Accepted Manuscript of an article published by Springer
発行日
2014-02
出版物タイトル
World Journal of Microbiology and Biotechnology
30巻
2号
出版者
Springer
開始ページ
681
終了ページ
691
ISSN
09593993
NCID
AA10811107
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1007/s11274-013-1501-3
プロジェクト
インド感染症共同研究