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ID 56687
フルテキストURL
著者
Imamura, Daisuke Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
Mizuno, Tamaki Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
Miyoshi, Shin‐ichi
Shinoda, Sumio Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
抄録
Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.
キーワード
Vibrio cholerae
resuscitation
viable but nonculturable
備考
This is an Accepted Manuscript of an article published by Wiley-Blackwell
発行日
2015-02-09
出版物タイトル
Microbiology and immunology
59巻
5号
出版者
Wiley-Blackwell
開始ページ
305
終了ページ
310
ISSN
03855600
NCID
AA00738350
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1111/1348-0421.12246
プロジェクト
インド感染症共同研究