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ID 56682
フルテキストURL
著者
Elgaml, Abdelaziz Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Miyoshi, Shin-ichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
抄録
Temperature is one of the important parameters regulating the expression of virulence factors in bacteria. The global regulator, a histone-like nucleoid structuring protein (H-NS), is known to play a crucial role in this regulation. In the present study, we first clarified the role of H-NS in the temperature-dependent regulation of virulence factor production in Vibrio vulnificus, including that of the cytolytic toxin (V. vulnificus hemolysin: VVH) and the proteolytic enzyme (V. vulnificus protease: VVP). The expression of hns itself was subjected to temperature regulation, where hns was expressed more at 26℃ than at 37℃. VVH production and the expression of its gene vvhA were increased by disruption of the hns gene. H-NS appeared to affect the vvhA expression by the well-documented transcriptional silencing mechanism. On the other hand, hns disruption resulted in the reduction of VVP production and the expression of its gene vvpE. H-NS was suggested to positively regulate vvpE expression through the increase in the level of the rpoS mRNA. Moreover, H-NS was found to contribute to the survival of V. vulnificus in stressful environments. When compared to the wild type strain, the hns mutant exhibited reduced survival rates when subjected to acidic pH, hyperosmotic and oxidative stress.
キーワード
Vibrio vulnificus
Temperature
H-NS
Hemolysin
Metalloprotease
Stress response
備考
Original
発行日
2015
出版物タイトル
Biocontrol science
20巻
4号
出版者
The Society for Antibacterial and Antifungal Agents
開始ページ
263
終了ページ
274
ISSN
13424815
NCID
AA11169621
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
Publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.4265/bio.20.263
プロジェクト
インド感染症共同研究