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ID 56657
フルテキストURL
著者
Imamura, Daisuke Collaborative Research Center of Okayama University for Infectious Diseases in India
Morita, Masatomo Department of Bacteriology I, National Institute of Infectious Diseases
Sekizuka, Tsuyoshi Pathogen Genomics Center, National Institute of Infectious Diseases
Mizuno, Tamaki Collaborative Research Center of Okayama University for Infectious Diseases in India
Takemura, Taichiro Vietnam Research Station, Institute of Tropical Medicine, Nagasaki University
Yamashiro, Tetsu Vietnam Research Station, Institute of Tropical Medicine, Nagasaki University
Chowdhury, Goutam Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Pazhani, Gururaja P. Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Mukhopadhyay, Asish K. Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Ramamurthy, Thandavarayan Translational Health Science and Technology Institute
Miyoshi, Shin-ichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kuroda, Makoto Pathogen Genomics Center, National Institute of Infectious Diseases
Shinoda, Sumio Collaborative Research Center of Okayama University for Infectious Diseases in India
Ohnishi, Makoto Department of Bacteriology I, National Institute of Infectious Diseases
抄録
Cholera is an acute diarrheal disease and a major public health problem in many developing countries in Asia, Africa, and Latin America. Since the Bay of Bengal is considered the epicenter for the seventh cholera pandemic, it is important to understand the genetic dynamism of Vibrio cholerae from Kolkata, as a representative of the Bengal region. We analyzed whole genome sequence data of V. cholerae O1 isolated from cholera patients in Kolkata, India, from 2007 to 2014 and identified the heterogeneous genomic region in these strains. In addition, we carried out a phylogenetic analysis based on the whole genome single nucleotide polymorphisms to determine the genetic lineage of strains in Kolkata. This analysis revealed the heterogeneity of the Vibrio seventh pandemic island (VSP)-II in Kolkata strains. The ctxB genotype was also heterogeneous and was highly related to VSP-II types. In addition, phylogenetic analysis revealed the shifts in predominant strains in Kolkata. Two distinct lineages, 1 and 2, were found between 2007 and 2010. However, the proportion changed markedly in 2010 and lineage 2 strains were predominant thereafter. Lineage 2 can be divided into four sublineages, I, II, III and IV. The results of this study indicate that lineages 1 and 2-I were concurrently prevalent between 2007 and 2009, and lineage 2-III observed in 2010, followed by the predominance of lineage 2-IV in 2011 and continued until 2014. Our findings demonstrate that the epidemic of cholera in Kolkata was caused by several distinct strains that have been constantly changing within the genetic lineages of V. cholerae O1 in recent years.
備考
This research is supported by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) from Ministry of Education, Culture, Sport, Science & Technology in Japan, and Japan Agency for Medical Research and Development (AMED).
発行日
2017-02-13
出版物タイトル
PLOS Neglected Tropical Diseases
11巻
2号
出版者
PLOS
開始ページ
e0005386
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1371/journal.pntd.0005386
プロジェクト
インド感染症共同研究