このエントリーをはてなブックマークに追加
ID 30739
JaLCDOI
フルテキストURL
著者
Aiga, Ayako Okayama University
Asaumi, Koji Okayama University
Lee, You Jin Chosun University
Kadota, Hiroaki Okayama University
Mitani, Shigeru Okayama University
Ozaki, Toshifumi Okayama University Kaken ID researchmap
Takigawa, Masaharu Okayama University Kaken ID publons researchmap
抄録
The localization and expression of neurotrophins and their receptors during distraction osteogenesis was investigated in 72 male rat femurs (11 weeks old) to further clarify the concurrence of cellular and molecular events of new bone formation. After osteotomy, a 7-day lag phase was followed by distraction at the rate of 0.25 mm/12 h for 21 days (distraction phase), and a 7-day consolidation phase. The localization of neurotrophins (NGF, BDNF and NT-3) and their receptors tropomyosinrelated kinases (TRKA, TRKB and TRKC) by immunostaining showed positive staining in bone forming cells in each stage, although the presence and staining intensity varied by cell type and phase. The expressions of NGF, BDNF and NT-3 by real-time polymerase chain reaction (real-time PCR) showed that the peak of the mRNA expression of NGF occurred 10 days after distraction. NT-3 increased during bone extension, but decreased when distraction stopped. In contrast, BDNF continued to increase gradually throughout the distraction and consolidation phases. These findings suggest that neurotrophins and their receptors may play different roles in endochondral and intramembranous ossification in distraction osteogenesis. The tension stress caused by distraction may stimulate the expression of neurotrophins and their receptors, and promote osteogenesis.
キーワード
neurotrophin
Trk
distraction osteogenesis
mechanical stress
Amo Type
Article
発行日
2006-10
出版物タイトル
Acta Medica Okayama
60巻
5号
出版者
Okayama University Medical School
開始ページ
267
終了ページ
277
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT